Construct: ORF TRCN0000492217

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012334.1_s317c1
Derived from:
ccsbBroadEn_01693
DNA Barcode:
GTGCTGCGACTTACACACAATCTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TNNT2 (7139)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492217
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7139 TNNT2 troponin T2, cardiac type NM_001001431.3 99.8% 100% 855A>G
2 human 7139 TNNT2 troponin T2, cardiac type XM_017002217.1 99.8% 100% 855A>G
3 human 7139 TNNT2 troponin T2, cardiac type XM_017002216.2 99.1% 99.3% 569_574delAGGCCC;861A>G
4 human 7139 TNNT2 troponin T2, cardiac type XM_024449455.1 99.1% 99.3% 569_574delAGGCCC;861A>G
5 human 7139 TNNT2 troponin T2, cardiac type NM_001001430.3 98.8% 98.9% 569_577delAGCAGGCCC;864A>G
6 human 7139 TNNT2 troponin T2, cardiac type NM_001276347.2 98.8% 98.9% 569_577delAGCAGGCCC;864A>G
7 human 7139 TNNT2 troponin T2, cardiac type XM_006711508.3 98.8% 98.9% 569_577delAGCAGGCCC;864A>G
8 human 7139 TNNT2 troponin T2, cardiac type XM_006711509.3 98.4% 98.6% 128_129insAGA;566_574delAGCAGGCCC;861A>G
9 human 7139 TNNT2 troponin T2, cardiac type XM_024449454.1 98.4% 98.6% 128_129insAGA;566_574delAGCAGGCCC;861A>G
10 human 7139 TNNT2 troponin T2, cardiac type NM_001001432.3 97.4% 97.5% 48_49insGAAGAAGCAGCTGTT;554_559delAGGCCC;846A>G
11 human 7139 TNNT2 troponin T2, cardiac type XM_011509942.2 97.1% 97.2% 48_49insGAAGAAGCAGCTGTT;554_562delAGCAGGCCC;849A>G
12 human 7139 TNNT2 troponin T2, cardiac type XM_011509943.2 97.1% 97.2% 48_49insGAAGAAGCAGCTGTT;554_562delAGCAGGCCC;849A>G
13 human 7139 TNNT2 troponin T2, cardiac type XM_011509944.2 96.7% 96.8% (many diffs)
14 human 7139 TNNT2 troponin T2, cardiac type NM_000364.4 96.4% 96.6% 67_96del;885A>G
15 human 7139 TNNT2 troponin T2, cardiac type XM_011509940.2 95.8% 95.9% 67_96del;599_604delAGGCCC;891A>G
16 human 7139 TNNT2 troponin T2, cardiac type NM_001276345.2 95.5% 95.6% 67_96del;599_607delAGCAGGCCC;894A>G
17 human 7139 TNNT2 troponin T2, cardiac type XM_011509938.2 95.5% 95.6% 67_96del;599_607delAGCAGGCCC;894A>G
18 human 7139 TNNT2 troponin T2, cardiac type XM_024449450.1 95.5% 95.6% 67_96del;599_607delAGCAGGCCC;894A>G
19 human 7139 TNNT2 troponin T2, cardiac type XM_011509941.2 95.5% 95.6% (many diffs)
20 human 7139 TNNT2 troponin T2, cardiac type XM_011509939.1 95.1% 95.3% (many diffs)
21 human 7139 TNNT2 troponin T2, cardiac type NM_001276346.2 82.9% 83% (many diffs)
22 human 7139 TNNT2 troponin T2, cardiac type XM_011509946.1 78.3% 78.4% 0_1ins177;392_400delAGCAGGCCC;687A>G
23 mouse 21956 Tnnt2 troponin T2, cardiac NM_001130178.2 83.8% 86.5% (many diffs)
24 mouse 21956 Tnnt2 troponin T2, cardiac NM_001130177.2 83.5% 85.5% (many diffs)
25 mouse 21956 Tnnt2 troponin T2, cardiac NM_011619.3 83.3% 88.5% (many diffs)
26 mouse 21956 Tnnt2 troponin T2, cardiac NM_001130176.1 82.7% 84.7% (many diffs)
27 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529393.3 82.7% 84.7% (many diffs)
28 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529394.3 82.7% 84.7% (many diffs)
29 mouse 21956 Tnnt2 troponin T2, cardiac NM_001130180.2 82.5% 87.6% (many diffs)
30 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529395.3 82.5% 87.6% (many diffs)
31 mouse 21956 Tnnt2 troponin T2, cardiac NM_001130174.2 81.9% 84.4% (many diffs)
32 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529391.3 81.1% 83.6% (many diffs)
33 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529392.3 81.1% 83.6% (many diffs)
34 mouse 21956 Tnnt2 troponin T2, cardiac NM_001130179.2 76.6% 79% (many diffs)
35 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529388.3 76.4% 78.2% (many diffs)
36 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529390.3 76.1% 80.6% (many diffs)
37 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529385.3 75.7% 77.5% (many diffs)
38 mouse 21956 Tnnt2 troponin T2, cardiac NM_001130181.2 75.4% 79.9% (many diffs)
39 mouse 21956 Tnnt2 troponin T2, cardiac NM_001130175.2 75% 77.2% (many diffs)
40 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529383.3 74.3% 76.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
921
ORF length:
855
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaag
61ttggcatgtc tgacatagaa gaggtggtgg aagagtacga ggaggaggag caggaagaag
121cagctgttga agagcaggag gaggcagcgg aagaggatgc tgaagcagag gctgagaccg
181aggagaccag ggcagaagaa gatgaagaag aagaggaagc aaaggaggct gaagatggcc
241caatggagga gtccaaacca aagcccaggt cgttcatgcc caacttggtg cctcccaaga
301tccccgatgg agagagagtg gactttgatg acatccaccg gaagcgcatg gagaaggacc
361tgaatgagtt gcaggcgctg atcgaggctc actttgagaa caggaagaaa gaggaggagg
421agctcgtttc tctcaaagac aggatcgaga gacgtcgggc agagcgggcc gagcagcagc
481gcatccggaa tgagcgggag aaggagcggc agaaccgCCT GGCTGAAGAG AGGGCTCGAC
541GAGAGGAGGA GGAGAACAGG AGGAAGGCTG AGGATGAGGC CCGGAAGAAG AAGGCTTTGT
601CCAACATGAT GCATTTTGGG GGTTACATCC AGAAGACAGA GCGGAAAAGT GGGAAGAGGC
661AGACTGAGCG GGAAAAGAAG AAGAAGATTC TGGCTGAGAG GAGGAAGGTG CTGGCCATTG
721ACCACCTGAA TGAAGATCAG CTGAGGGAGA AGGCCAAGGA GCTGTGGCAG AGCATCTATA
781ACTTGGAGGC AGAGAAGTTC GACCTGCAGG AGAAGTTCAA GCAGCAGAAA TATGAGATCA
841ATGTTCTCCG AAACAGGATC AACGATAACC AGAAAGTCTC CAAGACCCGC GGGAAGGCTA
901AAGTCACCGG GCGCTGGAAG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
961CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1021AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTGC TGCGACTTAC
1081ACACAATCTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)