Construct: ORF TRCN0000492228
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009314.1_s317c1
- Derived from:
- ccsbBroadEn_04039
- DNA Barcode:
- GAGGCCCTGTACATACAGATGGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C2orf49 (79074)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492228
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79074 | C2orf49 | chromosome 2 open reading f... | NM_024093.3 | 100% | 100% | |
| 2 | human | 79074 | C2orf49 | chromosome 2 open reading f... | XM_024453135.1 | 100% | 100% | |
| 3 | human | 79074 | C2orf49 | chromosome 2 open reading f... | NM_001286537.2 | 81.8% | 81.8% | 390_391ins126 |
| 4 | human | 79074 | C2orf49 | chromosome 2 open reading f... | XM_017004892.2 | 70.3% | 70.3% | 1_114del;504_505ins126 |
| 5 | human | 79074 | C2orf49 | chromosome 2 open reading f... | XM_024453136.1 | 70.3% | 70.3% | 1_114del;504_505ins126 |
| 6 | mouse | 98404 | AI597479 | expressed sequence AI597479 | NM_133818.1 | 85.4% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 762
- ORF length:
- 696
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gggggatgtg ggcggtcgca gctgcacgga ctcggaactg ctgctgcacc 121 cggagctgct gtcccaggag ttccttctcc tcactctgga gcagaagaac atagctgttg 181 aaactgatgt aagagtaaac aaagacagtc ttactgacct ttatgtccaa catgcaatac 241 cattgcctca gagggatttg ccgaagaata gatgggggaa aatgatGGAA AAGAAAAGAG 301 AACAACATGA GATTAAAAAT GAGACTAAAA GGAGTAGCAC TGTAGATGGG TTAAGGAAAA 361 GACCCCTCAT CGTATTTGAT GGAAGTTCAA CAAGTACAAG CATAAAAGTG AAAAAGACAG 421 AGAATGGAGA TAATGATCGA CTGAAGCCTC CCCCGCAGGC AAGCTTTACC AGTAATGCCT 481 TTAGAAAATT ATCAAATTCC TCTTCGAGTG TTTCACCCCT AATTTTGTCT TCCAATTTGC 541 CTGTGAACAA TAAAACGGAA CACAATAATA ATGACGCTAA ACAGAACCAT GACTTAACGC 601 ATAGGAAAAG TCCTTCAGGC CCTGTGAAGT CGCCACCATT GTCCCCTGTT GGAACTACTC 661 CAGTGAAGTT AAAGAGAGCT GCTCCTAAAG AAGAGGCAGA GGCCATGAAT AACCTGAAGC 721 CCCCACAAGC AAAAAGGAAG ATACAACATG TTACTTGGCC CTGCCCAACT TTCTTGTACA 781 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 841 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 901 AGGACGAGAG GCCCTGTACA TACAGATGGT AACGCGTTAA GTCgacaatc aacctctgga 961 ttacaaaatt tgtgaaagat t