Construct: ORF TRCN0000492231
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005778.1_s317c1
- Derived from:
- ccsbBroadEn_09072
- DNA Barcode:
- GGCGTTCTGCTTGCGCATATTTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDEL1 (81565)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492231
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81565 | NDEL1 | nudE neurodevelopment prote... | NM_030808.5 | 99.7% | 99.7% | 964C>A;966C>A;1023G>A |
| 2 | human | 81565 | NDEL1 | nudE neurodevelopment prote... | XM_017025185.1 | 99.7% | 99.7% | 964C>A;966C>A;1023G>A |
| 3 | human | 81565 | NDEL1 | nudE neurodevelopment prote... | XM_017025184.1 | 96% | 96% | (many diffs) |
| 4 | human | 81565 | NDEL1 | nudE neurodevelopment prote... | NM_001025579.3 | 93.2% | 91.8% | (many diffs) |
| 5 | human | 81565 | NDEL1 | nudE neurodevelopment prote... | XM_017025187.1 | 93.2% | 91.8% | (many diffs) |
| 6 | human | 81565 | NDEL1 | nudE neurodevelopment prote... | XM_017025186.1 | 89.8% | 88.5% | (many diffs) |
| 7 | human | 81565 | NDEL1 | nudE neurodevelopment prote... | XM_017025183.1 | 85% | 78.6% | (many diffs) |
| 8 | human | 81565 | NDEL1 | nudE neurodevelopment prote... | NM_001330129.2 | 77.4% | 76.5% | (many diffs) |
| 9 | human | 81565 | NDEL1 | nudE neurodevelopment prote... | XM_017025188.1 | 74.6% | 73.7% | (many diffs) |
| 10 | mouse | 83431 | Ndel1 | nudE neurodevelopment prote... | NM_023668.2 | 91.2% | 95.6% | (many diffs) |
| 11 | mouse | 83431 | Ndel1 | nudE neurodevelopment prote... | XM_006534540.1 | 85.2% | 87.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1101
- ORF length:
- 1035
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tggtgaagat ataccagatt tttcaagttt aaaggaggaa actgcttatt 121 ggaaggaact ttccttgaag tataagcaaa gcttccagga agctcgggat gagctagttg 181 aattccagga aggaagcaga gaattagaag cagagttgga ggcacaatta gtacaggctg 241 aacaaagaaa tagagacttg caggctgata accaaagact gaaatatgaa gtggaggcat 301 taaaggagaa gctagagcat caatatgcac agagctataa gcaggtctca gtgttagaag 361 atgatttaag tcagactcgg gccattaagg agcagttgca taagtatgtg agagagctgg 421 agcaggccaa cgacgacctg gagcgagcca aaagggcaac aatagtttca ctggaagact 481 ttgaacaaag gctaaaccag gccattgaac gaaatgcatt tttagaaagt gaacttgatg 541 aaaaggaatc tttgttggtc tctgtacaga ggttaaagga tgaagcaaga gatttaaggc 601 aagaactagc agttcgggaa agacaacagg aagtaactag aaagtcggct cctagctctc 661 caactctaga ctgtgaaaag atggacTCCG CCGTCCAAGC ATCACTTTCT TTGCCAGCTA 721 CCCCTGTTGG CAAAGGAACG GAGAACACTT TTCCTTCACC GAAAGCTATA CCAAATGGTT 781 TTGGTACCAG TCCACTAACT CCCTCTGCTA GGATATCAGC ACTAAACATC GTGGGGGATC 841 TCTTACGGAA AGTAGGGGCT TTAGAATCCA AATTAGCAGC TTGCAGGAAT TTTGCAAAGG 901 ACCAAGCATC ACGAAAATCC TATATTTCAG GGAATGTTAA CTGTGGGGTG CTGAATGGCA 961 ATGGCACAAA GTTCTCTCGA TCAGGGCATA CATCTTTCTT CGACAAAGGG GCAGTAAACG 1021 GCTTTGACAC AGCTCCTCCT CCTCCTGGTC TGGGCTCCTC GCGTCCATCG TCAGCGCCGG 1081 GTATGCTACC TCTCAGTGTG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1141 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1201 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGCG TTCTGCTTGC 1261 GCATATTTCC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt