Construct: ORF TRCN0000492233
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020340.2_s317c1
- DNA Barcode:
- GTTTTTGGCATTCGTGCCCAATAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRPS2 (5634)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492233
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5634 | PRPS2 | phosphoribosyl pyrophosphat... | NM_002765.4 | 99.8% | 100% | 45G>A |
| 2 | human | 5634 | PRPS2 | phosphoribosyl pyrophosphat... | NM_001039091.2 | 98.9% | 99% | 45G>A;305_313delAGGTAGGAG |
| 3 | human | 5631 | PRPS1 | phosphoribosyl pyrophosphat... | NM_002764.4 | 80% | 95.2% | (many diffs) |
| 4 | human | 221823 | PRPS1L1 | phosphoribosyl pyrophosphat... | NM_175886.3 | 79.3% | 90.8% | (many diffs) |
| 5 | human | 5631 | PRPS1 | phosphoribosyl pyrophosphat... | NM_001204402.1 | 28.9% | 34.9% | (many diffs) |
| 6 | mouse | 110639 | Prps2 | phosphoribosyl pyrophosphat... | NM_026662.5 | 88.9% | 98.7% | (many diffs) |
| 7 | mouse | 110639 | Prps2 | phosphoribosyl pyrophosphat... | NM_001313758.1 | 88.1% | 97.8% | (many diffs) |
| 8 | mouse | 110639 | Prps2 | phosphoribosyl pyrophosphat... | XM_017318352.1 | 36.8% | 23.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1026
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcccaac atcgtgctgt tcagcggcag ctcgcatcag gacctatccc 121 agcgcgtggc cgaccgcctg ggcctggagc tgggcaaggt ggtcacgaag aagttcagca 181 accaggagac cagcgtggag attggtgaaa gcgtgagagg ggaagatgtc tacatcatcc 241 agagcggctg cggggaaatt aacgacaacc tgatggaact cctcatcatg atcaatgcct 301 gcaagattgc gtcatcatcc agagtaactg ccgtgatccc gtgtttccca tacgcccgac 361 aagataaaaa ggacaagagt cgtgccccaa tttctgcaaa acttgtggcc aatatgctgt 421 cggtggctgg ggcggatcac atcatcacca tggacctgca tgcttctcag atacagggat 481 tctttgatat tcctgtggat aatttgtatg cggagcccgc agtcctgcag tggattcggg 541 aaaacattgc cgagtggaag aactgtatca ttgtttcacc tgacgcaggg ggagccaaaa 601 gggttacatc aattGCAGAC AGGTTGAATG TGGAATTTGC TTTGATCCAC AAAGAGAGGA 661 AGAAGGCGAA TGAAGTGGAC CGGATGGTCC TGGTGGGCGA CGTGAAGGAC CGTGTGGCCA 721 TCCTCGTGGA TGACATGGCT GACACTTGCG GCACCATCTG CCATGCTGCG GACAAGCTGC 781 TGTCAGCTGG AGCCACCAAA GTGTATGCTA TCCTTACCCA TGGGATCTTC TCTGGACCAG 841 CTATTTCCAG AATAAATAAT GCCGCCTTTG AGGCTGTTGT CGTCACAAAC ACAATTCCGC 901 AAGAGGACAA AATGAAACAC TGCACCAAGA TTCAGGTCAT TGACATTTCC ATGATCTTGG 961 CCGAAGCAAT CCGAAGGACA CACAATGGGG AATCCGTGTC CTACCTGTTC AGCCATGTCC 1021 CGCTATGAGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1081 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1141 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGTTTTT GGCATTCGTG CCCAATATAC 1201 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt