Construct: ORF TRCN0000492234
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021867.2_s317c1
- DNA Barcode:
- GTCATGTGGGACACTTACTGTTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- AADAT (51166)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492234
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51166 | AADAT | aminoadipate aminotransferase | NM_001286683.1 | 100% | 100% | |
| 2 | human | 51166 | AADAT | aminoadipate aminotransferase | NM_016228.4 | 100% | 100% | |
| 3 | human | 51166 | AADAT | aminoadipate aminotransferase | NM_182662.1 | 100% | 100% | |
| 4 | human | 51166 | AADAT | aminoadipate aminotransferase | XM_024454077.1 | 100% | 100% | |
| 5 | human | 51166 | AADAT | aminoadipate aminotransferase | NM_001286682.1 | 99% | 98.8% | 68_79del |
| 6 | human | 51166 | AADAT | aminoadipate aminotransferase | XM_006714231.2 | 91.5% | 91.5% | 1_117del |
| 7 | human | 51166 | AADAT | aminoadipate aminotransferase | XM_011532020.2 | 72.1% | 71.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1344
- ORF length:
- 1275
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gaattacgca cggttcatca cggcagcgag cgcagccaga aacccttctc 121 ccatccggac catgactgac atattgagca gaggaccaaa atcgatgatc tccttggctg 181 gtggcttacc aaatccaaac atgtttcctt ttaagactgc cgtaatcact gtagaaaatg 241 gaaagaccat ccaatttgga gaagagatga tgaagagagc acttcagtat tctccgagtg 301 ctggaattcc agagcttttg tcctggctaa aacagttaca aataaaattg cataatcctc 361 ctaccatcca ttacccaccc agtcaaggac aaatggatct atgtgtcaca tctggcagcc 421 aacaaggtct ttgtaaggtg tttgaaatga tcattaatcc tggagataat gtcctcctag 481 atgaacctgc ttattcagga actcttcaaa gtctgcaccc actgggctgc aacattatta 541 atgttgccag tgatgaaagt gggattgttc cagattccct aagagacata ctttccagat 601 ggaaaccaga agatgcaaag aatccccaga aaaacacccc caaatttctt tatactgttc 661 caaatggcaa caaccctact ggaaactcat taaccagtga acgcaaaaag gaaatctatg 721 agcttgcaag aaaatatgat ttcctcataa tagaagatga tccttactat tttctccagt 781 ttaacaagtt cagggtacca acatttcttt ccatggatgt tgatggacgt gtcatcagag 841 ctgactcttt ttcaaaaatc atttcctctg ggttgagaat aggattttta actggtccaa 901 aacccttaat agagagagtt attttacaca tacaagtttc aacattgcac cccagcactt 961 ttaaccagct catgatatca cagcttctac acgaatgggg agaagaaggt ttcatggcTC 1021 ATGTAGACAG GGTTATTGAT TTCTATAGTA ACCAGAAGGA TGCAATACTG GCAGCTGCAG 1081 ACAAGTGGTT AACTGGTTTG GCAGAATGGC ATGTTCCTGC TGCTGGAATG TTTTTATGGA 1141 TTAAAGTTAA AGGCATTAAT GATGTAAAAG AACTGATTGA AGAAAAGGCC GTTAAGATGG 1201 GGGTATTAAT GCTCCCTGGA AATGCTTTCT ACGTCGATAG CTCAGCTCCT AGCCCTTACT 1261 TGAGAGCATC CTTCTCTTCA GCTTCTCCAG AACAGATGGA TGTGGCCTTC CAGGTATTAG 1321 CACAACTTAT AAAAGAATCT TTATGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1381 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1441 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTCATGTG 1501 GGACACTTAC TGTTTAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1561 aagatt