Construct: ORF TRCN0000492273
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010716.1_s317c1
- Derived from:
- ccsbBroadEn_14192
- DNA Barcode:
- CTACTATATTAGCGCGTGCACTCC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MOB1A (55233)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492273
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55233 | MOB1A | MOB kinase activator 1A | NM_018221.5 | 99.8% | 98.6% | 637delT |
| 2 | human | 55233 | MOB1A | MOB kinase activator 1A | NM_001317110.2 | 99.3% | 98.1% | 14_15insCAG;634delT |
| 3 | human | 55233 | MOB1A | MOB kinase activator 1A | NM_001317111.1 | 82.9% | 81% | (many diffs) |
| 4 | human | 55233 | MOB1A | MOB kinase activator 1A | XM_024452973.1 | 82.9% | 81% | (many diffs) |
| 5 | human | 92597 | MOB1B | MOB kinase activator 1B | NM_173468.4 | 79.2% | 94.4% | (many diffs) |
| 6 | human | 92597 | MOB1B | MOB kinase activator 1B | NM_001244766.2 | 76.5% | 89.2% | (many diffs) |
| 7 | human | 92597 | MOB1B | MOB kinase activator 1B | XM_005265709.2 | 76.5% | 89.2% | (many diffs) |
| 8 | human | 92597 | MOB1B | MOB kinase activator 1B | XM_011532412.2 | 71.2% | 82.9% | (many diffs) |
| 9 | human | 55233 | MOB1A | MOB kinase activator 1A | NM_001317112.2 | 63.6% | 63.8% | 411A>T;414_414delTins234 |
| 10 | human | 92597 | MOB1B | MOB kinase activator 1B | NM_001244767.2 | 54% | 61.7% | (many diffs) |
| 11 | mouse | 232157 | Mob1a | MOB kinase activator 1A | NM_145571.2 | 92.9% | 98.6% | (many diffs) |
| 12 | mouse | 232157 | Mob1a | MOB kinase activator 1A | XM_006505969.3 | 92.4% | 98.1% | (many diffs) |
| 13 | mouse | 68473 | Mob1b | MOB kinase activator 1B | NM_026735.2 | 78.9% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 714
- ORF length:
- 648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cttcctcttc agcagccgct cttctaaaac attcaaacca aagaagaata 121 tccctgaagg atctcatcag tatgaactct taaaacatgc agaagcaact ctaggaagtg 181 ggaatctgag acaagctgtt atgttgcctG AGGGAGAGGA TCTCAATGAA TGGATTGCTG 241 TGAACACTGT GGATTTCTTT AACCAGATCA ACATGTTATA TGGAACTATT ACAGAATTCT 301 GCACTGAAGC AAGCTGTCCA GTCATGTCTG CAGGTCCGAG ATATGAATAT CACTGGGCAG 361 ATGGTACTAA TATTAAAAAG CCAATCAAAT GTTCTGCACC AAAATACATT GACTATTTGA 421 TGACTTGGGT TCAAGATCAG CTTGATGATG AAACTCTTTT TCCTTCTAAG ATTGGTGTCC 481 CATTTCCCAA AAACTTTATG TCTGTGGCAA AGACTATTCT AAAGCGTCTG TTCAGGGTTT 541 ATGCCCATAT TTATCACCAG CACTTTGATT CTGTGATGCA GCTGCAAGAG GAGGCCCACC 601 TCAACACCTC CTTTAAGCAC TTTATTTTCT TTGTTCAGGA GTTTAATCTG ATTGATAGGC 661 GTGAGCTGGC ACCTCTTCAA GAATTAATAG AGAAACTTGG ACAAAAGACA GATACCCAAC 721 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 781 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 841 ATCTTGTGGA AAGGACGACT ACTATATTAG CGCGTGCACT CCACGCGTTA AGTCgacaat 901 caacctctgg attacaaaat ttgtgaaaga tt