Construct: ORF TRCN0000492298
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020272.2_s317c1
- DNA Barcode:
- ACCTTTATGCCGATCAATACAAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CSNK2A2 (1459)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492298
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1459 | CSNK2A2 | casein kinase 2 alpha 2 | NM_001896.4 | 99.7% | 99.7% | 1050_1051insTTG |
| 2 | human | 1459 | CSNK2A2 | casein kinase 2 alpha 2 | XM_005255800.4 | 85.1% | 85.1% | 213_214ins153;897_898insTTG |
| 3 | human | 1459 | CSNK2A2 | casein kinase 2 alpha 2 | XM_017022945.2 | 65% | 61.9% | (many diffs) |
| 4 | human | 1459 | CSNK2A2 | casein kinase 2 alpha 2 | XM_005255801.3 | 60.6% | 60.6% | 0_1ins411;639_640insTTG |
| 5 | mouse | 13000 | Csnk2a2 | casein kinase 2, alpha prim... | NM_009974.3 | 94.5% | 98.5% | (many diffs) |
| 6 | mouse | 13000 | Csnk2a2 | casein kinase 2, alpha prim... | XM_017312556.1 | 89.7% | 93.7% | (many diffs) |
| 7 | mouse | 13000 | Csnk2a2 | casein kinase 2, alpha prim... | XM_006530641.3 | 80.1% | 84% | (many diffs) |
| 8 | mouse | 13000 | Csnk2a2 | casein kinase 2, alpha prim... | XM_006530642.1 | 56.7% | 59.5% | (many diffs) |
| 9 | mouse | 13000 | Csnk2a2 | casein kinase 2, alpha prim... | XM_006530643.3 | 56.7% | 59.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1125
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcccggc ccggccgcgg gcagcagggc ccgggtctac gccgaggtga 121 acagtctgag gagccgcgag tactgggact acgaggctca cgtcccgagc tggggtaatc 181 aagatgatta ccaactggtt cgaaaacttg gtcggggaaa atatagtgaa gtatttgagg 241 ccattaatat caccaacaat gagagagtgg ttgtaaaaat cctgaagcca gtgaagaaaa 301 agaagataaa acgagaggtt aagattctgg agaaccttcg tggtggaaca aatatcatta 361 agctgattga cactgtaaag gaccccgtgt caaagacacc agctttggta tttgaatata 421 tcaataatac agattttaag caactctacc agatcctgac agactttgat atccggtttt 481 atatgtatga actacttaaa gctctggatt actgccacag caagggaatc atgcacaggg 541 atgtgaaacc tcacaatgtc atgatagatc accaacagaa aaagctgcga ctgatagatt 601 ggggtctggc agaattctat catcctgctc aggagtacaa tgttcgtgta gcctcaaggt 661 acttcaaggg accagagctc ctcgtggact atcAGATGTA TGATTATAGC TTGGACATGT 721 GGAGTTTGGG CTGTATGTTA GCAAGCATGA TCTTTCGAAG GGAACCATTC TTCCATGGAC 781 AGGACAACTA TGACCAGCTT GTTCGCATTG CCAAGGTTCT GGGTACAGAA GAACTGTATG 841 GGTATCTGAA GAAGTATCAC ATAGACCTAG ATCCACACTT CAACGATATC CTGGGACAAC 901 ATTCACGGAA ACGCTGGGAA AACTTTATCC ATAGTGAGAA CAGACACCTT GTCAGCCCTG 961 AGGCCCTAGA TCTTCTGGAC AAACTTCTGC GATACGACCA TCAACAGAGA CTGACTGCCA 1021 AAGAGGCCAT GGAGCACCCA TACTTCTACC CTGTGGTGAA GGAGCAGTCC CAGCCTTGTG 1081 CAGACAATGC TGTGCTTTCC AGTGGTCTCA CGGCAGCACG ATTGTGAGAC CCAGCTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGAACCTTTA TGCCGATCAA TACAAATACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt