Construct: ORF TRCN0000492300

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009493.1_s317c1
Derived from:
ccsbBroadEn_00163
DNA Barcode:
CGTCGGGTCCTCGACTCTACATTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRDM1 (639)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492300
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 639 PRDM1 PR/SET domain 1 NM_182907.3 100% 100%
2 human 639 PRDM1 PR/SET domain 1 XM_011536064.3 96.5% 96.5% 0_1ins72
3 human 639 PRDM1 PR/SET domain 1 XM_011536063.2 87.4% 87.1% (many diffs)
4 human 639 PRDM1 PR/SET domain 1 XM_017011187.1 87.4% 87.1% (many diffs)
5 human 639 PRDM1 PR/SET domain 1 NM_001198.4 83.6% 83.3% (many diffs)
6 human 639 PRDM1 PR/SET domain 1 XM_011536062.3 82.2% 82% (many diffs)
7 human 639 PRDM1 PR/SET domain 1 XM_006715550.3 77.1% 76.8% (many diffs)
8 mouse 12142 Prdm1 PR domain containing 1, wit... XM_006512504.3 83.5% 85.6% (many diffs)
9 mouse 12142 Prdm1 PR domain containing 1, wit... XM_006512503.2 75.8% 77.3% (many diffs)
10 mouse 12142 Prdm1 PR domain containing 1, wit... XM_011243109.1 75.8% 77.3% (many diffs)
11 mouse 12142 Prdm1 PR domain containing 1, wit... NM_007548.4 72.7% 74.1% (many diffs)
12 mouse 12142 Prdm1 PR domain containing 1, wit... XM_006512502.3 71.4% 72.8% (many diffs)
13 mouse 12142 Prdm1 PR domain containing 1, wit... XM_006512505.2 69.9% 71.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2142
ORF length:
2073
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggaaaagatc tattccagag gggagcttca ccacttcatt gacggcttta
121atgaagagaa aagcaactgg atgcgctatg tgaatccagc acactctccc cgggagcaaa
181acctggctgc gtgtcagaac gggatgaaca tctacttcta caccattaag cccatccctg
241ccaaccagga acttcttgtg tggtattgtc gggactttgc agaaaggctt cactaccctt
301atcccggaga gctgacaatg atgaatctca cacaaacaca gagcagtcta aagcaaccga
361gcactgagaa aaatgaactc tgcccaaaga atgtcccaaa gagagagtac agcgtgaaag
421aaatcctaaa attggactcc aacccctcca aaggaaagga cctctaccgt tctaacattt
481cacccctcac atcagaaaag gacctcgatg actttagaag acgtgggagc cccgaaatgc
541ccttctaccc tcgggtcgtt taccccatcc gggcccctct gccagaagac tttttgaaag
601cttccctggc ctacgggatc gagagaccca cgtacatcac tcgctccccc attccatcct
661ccaccactcc aagcccctct gcaagaagca gccccgacca aagcctcaag agctccagcc
721ctcacagcag ccctgggaat acggtgtccc ctgtgggccc cggctctcaa gagcaccggg
781actcctacgc ttacttgaac gcgtcctacg gcacggaagg tttgggctcc taccctggct
841acgcacccct gccccacctc ccgccagctt tcatcccctc gtacaacgct cactacccca
901agttcctctt gcccccctac ggcatgaatt gtaatggcct gagcgctgtg agcagcatga
961atggcatcaa caactttggc ctcttcccga ggctgtgccc tgtctacagc aatctcctcg
1021gtgggggcag cctgccccac cccatgctca accccacttc tctcccgagc tcgctgccct
1081cagatggagc ccggaggttg ctccagccgg agcatcccag ggaggtgctt gtcccggcgc
1141cccacagtgc cttctccttt accggggccg ccgccagcat gaaggacaag gcctgtagcc
1201ccacaagcgg gtctcccacg gcgggaacag ccgccacggc agaacatgtg gtgcagccca
1261aagctacctc agcagcgatg gcagccccca gcagcgacga agccatgaat ctcattaaaa
1321acaaaagaaa catgaccggc tacaagaccc ttccctaccc gctgaagaag cagaacggca
1381agatcaagta cgaatgcaac gtttgcgcca agactttcgg ccagctctcc aatctgaagg
1441tccacctgag agtgcacagt ggagaacggc ctttcaaatg tcagacttgc aacaagggct
1501ttactcagct cgcccacctg cagaaacact acctggtaca cacgggagaa aagccacatg
1561aatgccaggt ctgccacaag agatttagca gcaccagcaa tctcaagacc cacctgcgac
1621tccattctgg agagaaacca taccaatgca aggtgtgccc tgccaagttc acccagtttg
1681tgcacctgaa actgcacaag cgtctgcaca cccgggagcg gccccacaag tgctcccagt
1741gccacaagaa ctacatccat ctctgtagcc tcaaggttca cctgaaaggg aactgcgctg
1801cggccccggc gcctgggctg cccttggaag atctgacccg aatcaatgaa gaaatcgaga
1861agtttgacat cagtgacaat gctgaccggc tcgaggacgt ggaggatgac atcagtgtga
1921tctctgTAGT GGAGAAGGAA ATTCTGGCCG TGGTCAGAAA AGAGAAAGAA GAAACTGGCC
1981TGAAAGTGTC TTTGCAAAGA AACATGGGGA ATGGACTCCT CTCCTCAGGG TGCAGCCTTT
2041ATGAGTCATC AGATCTACCC CTCATGAAGT TGCCTCCCAG CAACCCACTA CCTCTGGTAC
2101CTGTAAAGGT CAAACAAGAA ACAGTTGAAC CAATGGATCC TTTGCCAACT TTCTTGTACA
2161AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
2221AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
2281AGGACGACGT CGGGTCCTCG ACTCTACATT AACGCGTTAA GTCgacaatc aacctctgga
2341ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)