Construct: ORF TRCN0000492300
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009493.1_s317c1
- Derived from:
- ccsbBroadEn_00163
- DNA Barcode:
- CGTCGGGTCCTCGACTCTACATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRDM1 (639)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492300
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 639 | PRDM1 | PR/SET domain 1 | NM_182907.3 | 100% | 100% | |
| 2 | human | 639 | PRDM1 | PR/SET domain 1 | XM_011536064.3 | 96.5% | 96.5% | 0_1ins72 |
| 3 | human | 639 | PRDM1 | PR/SET domain 1 | XM_011536063.2 | 87.4% | 87.1% | (many diffs) |
| 4 | human | 639 | PRDM1 | PR/SET domain 1 | XM_017011187.1 | 87.4% | 87.1% | (many diffs) |
| 5 | human | 639 | PRDM1 | PR/SET domain 1 | NM_001198.4 | 83.6% | 83.3% | (many diffs) |
| 6 | human | 639 | PRDM1 | PR/SET domain 1 | XM_011536062.3 | 82.2% | 82% | (many diffs) |
| 7 | human | 639 | PRDM1 | PR/SET domain 1 | XM_006715550.3 | 77.1% | 76.8% | (many diffs) |
| 8 | mouse | 12142 | Prdm1 | PR domain containing 1, wit... | XM_006512504.3 | 83.5% | 85.6% | (many diffs) |
| 9 | mouse | 12142 | Prdm1 | PR domain containing 1, wit... | XM_006512503.2 | 75.8% | 77.3% | (many diffs) |
| 10 | mouse | 12142 | Prdm1 | PR domain containing 1, wit... | XM_011243109.1 | 75.8% | 77.3% | (many diffs) |
| 11 | mouse | 12142 | Prdm1 | PR domain containing 1, wit... | NM_007548.4 | 72.7% | 74.1% | (many diffs) |
| 12 | mouse | 12142 | Prdm1 | PR domain containing 1, wit... | XM_006512502.3 | 71.4% | 72.8% | (many diffs) |
| 13 | mouse | 12142 | Prdm1 | PR domain containing 1, wit... | XM_006512505.2 | 69.9% | 71.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2142
- ORF length:
- 2073
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaaagatc tattccagag gggagcttca ccacttcatt gacggcttta 121 atgaagagaa aagcaactgg atgcgctatg tgaatccagc acactctccc cgggagcaaa 181 acctggctgc gtgtcagaac gggatgaaca tctacttcta caccattaag cccatccctg 241 ccaaccagga acttcttgtg tggtattgtc gggactttgc agaaaggctt cactaccctt 301 atcccggaga gctgacaatg atgaatctca cacaaacaca gagcagtcta aagcaaccga 361 gcactgagaa aaatgaactc tgcccaaaga atgtcccaaa gagagagtac agcgtgaaag 421 aaatcctaaa attggactcc aacccctcca aaggaaagga cctctaccgt tctaacattt 481 cacccctcac atcagaaaag gacctcgatg actttagaag acgtgggagc cccgaaatgc 541 ccttctaccc tcgggtcgtt taccccatcc gggcccctct gccagaagac tttttgaaag 601 cttccctggc ctacgggatc gagagaccca cgtacatcac tcgctccccc attccatcct 661 ccaccactcc aagcccctct gcaagaagca gccccgacca aagcctcaag agctccagcc 721 ctcacagcag ccctgggaat acggtgtccc ctgtgggccc cggctctcaa gagcaccggg 781 actcctacgc ttacttgaac gcgtcctacg gcacggaagg tttgggctcc taccctggct 841 acgcacccct gccccacctc ccgccagctt tcatcccctc gtacaacgct cactacccca 901 agttcctctt gcccccctac ggcatgaatt gtaatggcct gagcgctgtg agcagcatga 961 atggcatcaa caactttggc ctcttcccga ggctgtgccc tgtctacagc aatctcctcg 1021 gtgggggcag cctgccccac cccatgctca accccacttc tctcccgagc tcgctgccct 1081 cagatggagc ccggaggttg ctccagccgg agcatcccag ggaggtgctt gtcccggcgc 1141 cccacagtgc cttctccttt accggggccg ccgccagcat gaaggacaag gcctgtagcc 1201 ccacaagcgg gtctcccacg gcgggaacag ccgccacggc agaacatgtg gtgcagccca 1261 aagctacctc agcagcgatg gcagccccca gcagcgacga agccatgaat ctcattaaaa 1321 acaaaagaaa catgaccggc tacaagaccc ttccctaccc gctgaagaag cagaacggca 1381 agatcaagta cgaatgcaac gtttgcgcca agactttcgg ccagctctcc aatctgaagg 1441 tccacctgag agtgcacagt ggagaacggc ctttcaaatg tcagacttgc aacaagggct 1501 ttactcagct cgcccacctg cagaaacact acctggtaca cacgggagaa aagccacatg 1561 aatgccaggt ctgccacaag agatttagca gcaccagcaa tctcaagacc cacctgcgac 1621 tccattctgg agagaaacca taccaatgca aggtgtgccc tgccaagttc acccagtttg 1681 tgcacctgaa actgcacaag cgtctgcaca cccgggagcg gccccacaag tgctcccagt 1741 gccacaagaa ctacatccat ctctgtagcc tcaaggttca cctgaaaggg aactgcgctg 1801 cggccccggc gcctgggctg cccttggaag atctgacccg aatcaatgaa gaaatcgaga 1861 agtttgacat cagtgacaat gctgaccggc tcgaggacgt ggaggatgac atcagtgtga 1921 tctctgTAGT GGAGAAGGAA ATTCTGGCCG TGGTCAGAAA AGAGAAAGAA GAAACTGGCC 1981 TGAAAGTGTC TTTGCAAAGA AACATGGGGA ATGGACTCCT CTCCTCAGGG TGCAGCCTTT 2041 ATGAGTCATC AGATCTACCC CTCATGAAGT TGCCTCCCAG CAACCCACTA CCTCTGGTAC 2101 CTGTAAAGGT CAAACAAGAA ACAGTTGAAC CAATGGATCC TTTGCCAACT TTCTTGTACA 2161 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2221 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2281 AGGACGACGT CGGGTCCTCG ACTCTACATT AACGCGTTAA GTCgacaatc aacctctgga 2341 ttacaaaatt tgtgaaagat t