Construct: ORF TRCN0000492322

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021409.2_s317c1
DNA Barcode:
TACACAATCAATTACGAAATACCT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
PAK1 (5058)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492322
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5058 PAK1 p21 (RAC1) activated kinase 1 NM_002576.4 82.3% 82.3% 598_885del
2 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_017017846.1 82.3% 82.3% 598_885del
3 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_017017847.2 82.3% 82.3% 598_885del
4 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_017017848.1 82.3% 82.3% 598_885del
5 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_017017849.1 82.3% 82.3% 598_885del
6 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_017017850.1 82.3% 82.3% 598_885del
7 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_024448560.1 82.3% 82.3% 598_885del
8 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_024448561.1 82.3% 82.3% 598_885del
9 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_024448562.1 82.3% 82.3% 598_885del
10 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_024448563.1 82.3% 82.3% 598_885del
11 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_024448564.1 82.3% 82.3% 598_885del
12 human 5058 PAK1 p21 (RAC1) activated kinase 1 NM_001128620.1 78.5% 77% 598_885del;1551_1599del;1659_1660ins25
13 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_011545080.3 78.5% 77% 598_885del;1551_1599del;1659_1660ins25
14 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_011545081.2 78.5% 77% 598_885del;1551_1599del;1659_1660ins25
15 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_011545082.2 78.5% 77% 598_885del;1551_1599del;1659_1660ins25
16 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_011545083.2 78.5% 77% 598_885del;1551_1599del;1659_1660ins25
17 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_011545084.3 78.5% 77% 598_885del;1551_1599del;1659_1660ins25
18 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_024448557.1 78.5% 77% 598_885del;1551_1599del;1659_1660ins25
19 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_024448558.1 78.5% 77% 598_885del;1551_1599del;1659_1660ins25
20 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_024448559.1 78.5% 77% 598_885del;1551_1599del;1659_1660ins25
21 mouse 18479 Pak1 p21 protein (Cdc42/Rac)-act... NM_011035.2 75.9% 81.4% (many diffs)
22 mouse 18479 Pak1 p21 protein (Cdc42/Rac)-act... XM_017322028.1 75.9% 81.4% (many diffs)
23 mouse 18479 Pak1 p21 protein (Cdc42/Rac)-act... XM_006507434.1 74.8% 80.2% (many diffs)
24 mouse 18479 Pak1 p21 protein (Cdc42/Rac)-act... XM_006507436.3 74.8% 80.2% (many diffs)
25 mouse 18479 Pak1 p21 protein (Cdc42/Rac)-act... XM_006507437.2 74.8% 80.2% (many diffs)
26 mouse 18479 Pak1 p21 protein (Cdc42/Rac)-act... XM_011241694.1 74.8% 80.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
96
ORF end:
1443
ORF length:
1347
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg
61cggccgcccc cttcaccgga tccctggtgg tgacaatgtc aaataacggc ctagacattc
121aagacaaacc cccagcccct ccgatgagaa ataccagcac tatgattgga gccggcagca
181aagatgctgg aaccctaaac catggttcta aacctctgcc tccaaaccca gaggagaaga
241aaaagaagga ccgattttac cgatccattt tacctggaga taaaacaaat aaaaagaaag
301agaaagagcg gccagagatt tctctccctt cagattttga acacacaatt catgtcggtt
361ttgatgctgt cacaggggag tttacgggaa tgccagagca gtgggcccgc ttgcttcaga
421catcaaatat cactaagtcg gagcagaaga aaaacccgca ggctgttctg gatgtgttgg
481agttttacaa ctcgaagaag acatccaaca gccagaaata catgagcttt acagataagt
541cagctgagga ttacaattct tctaatgcct tgaatgtgaa ggctgtgtct gagactcctg
601cagtgccacc agtttcagaa gatgaggatg atgatgatga tgatgctacc ccaccaccag
661tgattgctcc acgcccagag cacacaaaat ctgtggccat taagcagatg aatcttcagc
721agcagcccaa gaaagagctg attattaatg agatcctggt catgagggaa aacaagaacc
781caaacattgt gaattacttg gacagttacc tcgtgggaga tgagctgtgg gttgttatgg
841aatacttggc tggaggctcc ttgacagatg tggtgacaga aacttgcatg gatgaaggcc
901aaattgcagc tgtgtgccgt gagtgtctgc aggctctgga gttcttgcat tcgaaccagg
961tcattcacag agacatcaag agtgacaata ttctgttggg aatggatggc tctgtcaagc
1021taactgactt tGGATTCTGT GCACAGATAA CCCCAGAGCA GAGCAAACGG AGCACCATGG
1081TAGGAACCCC ATACTGGATG GCACCAGAGG TTGTGACACG AAAGGCCTAT GGGCCCAAGG
1141TTGACATCTG GTCCCTGGGC ATCATGGCCA TCGAAATGAT TGAAGGGGAG CCTCCATACC
1201TCAATGAAAA CCCTCTGAGA GCCTTGTACC TCATTGCCAC CAATGGGACC CCAGAACTTC
1261AGAACCCAGA GAAGCTGTCA GCTATCTTCC GGGACTTTCT GAACCGCTGT CTCGAGATGG
1321ATGTGGAGAA GAGAGGTTCA GCTAAAGAGC TGCTACAGCA TCAATTCCTG AAGATTGCCA
1381AGCCCCTCTC CAGCCTCACT CCACTGATTG CTGCAGCTAA GGAGGCAACA AAGAACAATC
1441ACTAAAAGGG TGGGCGCGCC GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1501CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1561AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATACA CAATCAATTA
1621CGAAATACCT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)