Construct: ORF TRCN0000492328
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021553.2_s317c1
- DNA Barcode:
- ACTGGCGTTCCAAATGCTACTAGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- THRA (7067)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492328
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7067 | THRA | thyroid hormone receptor alpha | NM_001190919.1 | 100% | 100% | |
| 2 | human | 7067 | THRA | thyroid hormone receptor alpha | NM_003250.6 | 100% | 100% | |
| 3 | human | 7067 | THRA | thyroid hormone receptor alpha | NM_001190918.2 | 92% | 92% | 1108_1109ins117 |
| 4 | human | 7067 | THRA | thyroid hormone receptor alpha | NM_199334.5 | 81.9% | 77.6% | (many diffs) |
| 5 | mouse | 21833 | Thra | thyroid hormone receptor alpha | NM_001313983.1 | 95.6% | 97.1% | (many diffs) |
| 6 | mouse | 21833 | Thra | thyroid hormone receptor alpha | NM_178060.4 | 78.4% | 77% | (many diffs) |
| 7 | mouse | 21833 | Thra | thyroid hormone receptor alpha | XR_001779972.1 | 44.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1542
- ORF length:
- 1470
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaacag aagccaagca aggtggagtg tgggtcagac ccagaggaga 121 acagtgccag gtcaccagat ggaaagcgaa aaagaaagaa cggccaatgt tccctgaaaa 181 ccagcatgtc agggtatatc cctagttacc tggacaaaga cgagcagtgt gtcgtgtgtg 241 gggacaaggc aactggttat cactaccgct gtatcacttg tgagggctgc aagggcttct 301 ttcgccgcac aatccagaag aacctccatc ccacctattc ctgcaaatat gacagctgct 361 gtgtcattga caagatcacc cgcaatcagt gccagctgtg ccgcttcaag aagtgcatcg 421 ccgtgggcat ggccatggac ttggttctag atgactcgaa gcgggtggcc aagcgtaagc 481 tgattgagca gaaccgggag cggcggcgga aggaggagat gatccgatca ctgcagcagc 541 gaccagagcc cactcctgaa gagtgggatc tgatccacat tgccacagag gcccatcgca 601 gcaccaatgc ccagggcagc cattggaaac agaggcggaa attcctgccc gatgacattg 661 gccagtcacc cattgtctcc atgccggacg gagacaaggt ggacctggaa gccttcagcg 721 agtttaccaa gatcatcacc ccggccatca cccgtgtggt ggactttgcc aaaaaactgc 781 ccatgttctc cgagctgcct tgcgaagacc agatcatcct cctgaagggg tgctgcatgg 841 agatcatgtc cctgcgggcg gctgtccgct acgaccctga gagcgacacc ctgacgctga 901 gtggggagat ggctgtcaag cgggagcagc tcaagaatgg cggcctgggc gtagtctccg 961 acgccatctt tgaactgggc aagtcactct ctgcctttaa cctggatgac acggaagtgg 1021 ctctgctgca ggctgtgctg ctaatgtcaa cagaccgctc gggcctgctg tgtgtggaca 1081 agatcgagaa gagtcaggag gcgtacctgc tggcgttcGA GCACTACGTC AACCACCGCA 1141 AACACAACAT TCCGCACTTC TGGCCCAAGC TGCTGATGAA GGAGAGAGAA GTGCAGAGTT 1201 CGATTCTGTA CAAGGGGGCA GCGGCAGAAG GCCGGCCGGG CGGGTCACTG GGCGTCCACC 1261 CGGAAGGACA GCAGCTTCTC GGAATGCATG TTGTTCAGGG TCCGCAGGTC CGGCAGCTTG 1321 AGCAGCAGCT TGGTGAAGCG GGAAGTCTCC AAGGGCCGGT TCTTCAGCAC CAGAGCCCGA 1381 AGAGCCCGCA GCAGCGTCTC CTGGAGCTGC TCCACCGAAG CGGAATTCTC CATGCCCGAG 1441 CGGTCTGTGG GGAAGACGAC AGCAGTGAGG CGGACTCCCC GAGCTCCTCT GAGGAGGAAC 1501 CGGAGGTCTG CGAGGACCTG GCAGGCAATG CAGCCTCTCC CTAGAACCCA GCTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA ACTGGCGTTC CAAATGCTAC TAGAACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt