Transcript: Human NM_199334.5

Homo sapiens thyroid hormone receptor alpha (THRA), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
THRA (7067)
Length:
5204
CDS:
426..1658

Additional Resources:

NCBI RefSeq record:
NM_199334.5
NBCI Gene record:
THRA (7067)

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_199334.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000020310 GTCAGGGTATATCCCTAGTTA pLKO.1 542 CDS 100% 5.625 7.875 N THRA n/a
2 TRCN0000343784 GTCAGGGTATATCCCTAGTTA pLKO_005 542 CDS 100% 5.625 7.875 N THRA n/a
3 TRCN0000020311 CAGCGAGTTTACCAAGATCAT pLKO.1 1070 CDS 100% 4.950 3.465 N THRA n/a
4 TRCN0000343721 CAGCGAGTTTACCAAGATCAT pLKO_005 1070 CDS 100% 4.950 3.465 N THRA n/a
5 TRCN0000222365 GCCATGGACTTGGTTCTAGAT pLKO.1 786 CDS 100% 4.950 3.465 N Thra n/a
6 TRCN0000020312 CAAACACAACATTCCGCACTT pLKO.1 1493 CDS 100% 4.050 2.835 N THRA n/a
7 TRCN0000020309 GCGTAAGCTGATTGAGCAGAA pLKO.1 827 CDS 100% 4.050 2.835 N THRA n/a
8 TRCN0000343720 GCGTAAGCTGATTGAGCAGAA pLKO_005 827 CDS 100% 4.050 2.835 N THRA n/a
9 TRCN0000020313 GTCACCAGATGGAAAGCGAAA pLKO.1 485 CDS 100% 4.050 2.835 N THRA n/a
10 TRCN0000343783 GTCACCAGATGGAAAGCGAAA pLKO_005 485 CDS 100% 4.050 2.835 N THRA n/a
11 TRCN0000222366 GAGGTCTTTGAGGATCAGGAA pLKO.1 1632 CDS 100% 2.640 1.848 N Thra n/a
12 TRCN0000027080 TGCCGCTTCAAGAAGTGCATT pLKO.1 753 CDS 100% 4.950 3.465 N Thra n/a
13 TRCN0000166364 CACACACACACACACACACAA pLKO.1 3435 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_199334.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000492328 ACTGGCGTTCCAAATGCTACTAGA pLX_317 29.1% 81.9% 77.6% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_07066 pDONR223 100% 81.8% 77.4% None (many diffs) n/a
3 ccsbBroad304_07066 pLX_304 0% 81.8% 77.4% V5 (not translated due to frame shift) (many diffs) n/a
4 TRCN0000478935 CAGCACGGCCAGTTGTCAAACAGA pLX_317 27.2% 81.8% 77.4% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000489623 TGTTACCACGGAGGCGAGAACGCA pLX_317 21.1% 81.8% 77.4% V5 (many diffs) n/a
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