Transcript: Human NM_003250.6

Homo sapiens thyroid hormone receptor alpha (THRA), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-29
Taxon:
Homo sapiens (human)
Gene:
THRA (7067)
Length:
2383
CDS:
426..1898

Additional Resources:

NCBI RefSeq record:
NM_003250.6
NBCI Gene record:
THRA (7067)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_003250.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000020310 GTCAGGGTATATCCCTAGTTA pLKO.1 542 CDS 100% 5.625 7.875 N THRA n/a
2 TRCN0000343784 GTCAGGGTATATCCCTAGTTA pLKO_005 542 CDS 100% 5.625 7.875 N THRA n/a
3 TRCN0000020311 CAGCGAGTTTACCAAGATCAT pLKO.1 1070 CDS 100% 4.950 3.465 N THRA n/a
4 TRCN0000343721 CAGCGAGTTTACCAAGATCAT pLKO_005 1070 CDS 100% 4.950 3.465 N THRA n/a
5 TRCN0000222365 GCCATGGACTTGGTTCTAGAT pLKO.1 786 CDS 100% 4.950 3.465 N Thra n/a
6 TRCN0000020312 CAAACACAACATTCCGCACTT pLKO.1 1493 CDS 100% 4.050 2.835 N THRA n/a
7 TRCN0000020309 GCGTAAGCTGATTGAGCAGAA pLKO.1 827 CDS 100% 4.050 2.835 N THRA n/a
8 TRCN0000343720 GCGTAAGCTGATTGAGCAGAA pLKO_005 827 CDS 100% 4.050 2.835 N THRA n/a
9 TRCN0000020313 GTCACCAGATGGAAAGCGAAA pLKO.1 485 CDS 100% 4.050 2.835 N THRA n/a
10 TRCN0000343783 GTCACCAGATGGAAAGCGAAA pLKO_005 485 CDS 100% 4.050 2.835 N THRA n/a
11 TRCN0000027080 TGCCGCTTCAAGAAGTGCATT pLKO.1 753 CDS 100% 4.950 3.465 N Thra n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_003250.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000492328 ACTGGCGTTCCAAATGCTACTAGA pLX_317 29.1% 100% 100% V5 (not translated due to prior stop codon) n/a
2 ccsbBroadEn_07066 pDONR223 100% 99.9% 99.7% None 1470_1471insC n/a
3 ccsbBroad304_07066 pLX_304 0% 99.9% 99.7% V5 (not translated due to frame shift) 1470_1471insC n/a
4 TRCN0000478935 CAGCACGGCCAGTTGTCAAACAGA pLX_317 27.2% 99.9% 99.7% V5 (not translated due to prior stop codon) 1470_1471insC n/a
5 TRCN0000489623 TGTTACCACGGAGGCGAGAACGCA pLX_317 21.1% 99.9% 99.7% V5 1470_1471insG n/a
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