Construct: ORF TRCN0000492353
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003118.1_s317c1
- Derived from:
- ccsbBroadEn_08229
- DNA Barcode:
- GAATGTATTCGATCCTGATCAGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCTD3 (51133)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492353
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51133 | KCTD3 | potassium channel tetrameri... | NM_016121.5 | 99.9% | 100% | 2322G>A |
| 2 | human | 51133 | KCTD3 | potassium channel tetrameri... | NM_001319294.2 | 99.7% | 99.7% | 1746_1747insGATGTA;2316G>A |
| 3 | human | 51133 | KCTD3 | potassium channel tetrameri... | XM_005273158.2 | 87.4% | 87.2% | (many diffs) |
| 4 | human | 51133 | KCTD3 | potassium channel tetrameri... | NM_001319295.2 | 87.1% | 86.9% | (many diffs) |
| 5 | mouse | 226823 | Kctd3 | potassium channel tetrameri... | NM_172650.2 | 83.7% | 93.5% | (many diffs) |
| 6 | mouse | 226823 | Kctd3 | potassium channel tetrameri... | XM_006497143.2 | 83.5% | 93.3% | (many diffs) |
| 7 | mouse | 226823 | Kctd3 | potassium channel tetrameri... | XM_011238911.2 | 75.5% | 82.8% | (many diffs) |
| 8 | mouse | 226823 | Kctd3 | potassium channel tetrameri... | XM_006497144.3 | 73.7% | 78.6% | (many diffs) |
| 9 | mouse | 226823 | Kctd3 | potassium channel tetrameri... | XM_017320169.1 | 55.3% | 61.7% | (many diffs) |
| 10 | mouse | 226823 | Kctd3 | potassium channel tetrameri... | XM_017320173.1 | 55.1% | 61.5% | (many diffs) |
| 11 | mouse | 226823 | Kctd3 | potassium channel tetrameri... | XM_017320175.1 | 47.5% | 53.4% | (many diffs) |
| 12 | mouse | 226823 | Kctd3 | potassium channel tetrameri... | XM_017320182.1 | 47.3% | 53.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2514
- ORF length:
- 2445
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgggaggg cactgcggca gcttccccgc ggcggcggcc ggcagcggcg 121 agatcgtcca actgaacgta ggggggacca gatttagtac ctcaagacaa actcttatgt 181 ggattccaga ttcttttttt tccagtttgc tgagtgggag aatttcaaca cttcgagatg 241 aaactggtgc tatatttatt gatagagatc cagcagcatt tgcacccatt ttaaattttc 301 ttcggacaaa agaactagac ttaaggggag tgagtattaa tgttctcagg catgaagcag 361 aattttacgg gatcactcca ttagtaagaa ggcttctctt atgtgaagaa ttggagcgtt 421 cctcttgtgg cagtgtcctt tttcatggtt acttgccccc accaggtatt cctagtcgta 481 aaataaacaa cacagtcaga tctgctgatt ctaggaatgg tctaaattct acagaaggtg 541 aagcccgggg aaatggtaca cagcctgttc tctctggaac gggagaagaa actgttaggc 601 taggatttcc tgtggatcca cgaaaggtgc taatagtagc tggccatcac aactggattg 661 tagctgcata tgcccatttt gctgtgtgtt acagaatcaa agaatcttca ggatggcagc 721 aagtgtttac gagcccatat ttggattgga ctatcgaacg agtagcttta aatgcaaagg 781 tggttggagg gccacatgga gacaaagaca aaatggttgc tgttgcctca gagagtagca 841 tcatcttgtg gagtgttcag gatgggggaa gtggaagtga aattggagtg ttcagcctgg 901 gtgttcctgt agatgctctc ttctttattg gtaaccagtt ggtggccacg agtcatacag 961 ggaaagtggg agtgtggaat gctgtcactc agcactggca ggttcaagat gttgttccta 1021 taactagtta tgacactgct ggatcattcc ttctgcttgg atgtaacaat ggatcaatat 1081 attacataga tatgcagaag ttccccttgc gaatgaaaga taatgatctt cttgtaactg 1141 aactgtatca tgatccttca aatgatgcta ttactgctct gagtgtttac ctcacaccca 1201 aaacaagtgt cagtggtaac tggatcgaga tcgcctatgg tacgagctct ggagcagtac 1261 gagtgattgt acaacaccca gagacagttg ggtcaggtcc tcagcttttt cagactttca 1321 cagttcaccg aagtcccgta acaaaaatca tgctatcaga gaagcatctt gtatcagtct 1381 gtgcagataa taatcatgtc cggacgtgga cagtaacacg attcagagga atgatctcta 1441 ctcagccagg ttctactcct ttagcgtcat tcaagatact atccctggag gagacagaaa 1501 gtcatggtag ctattcctct ggaaatgaca taggaccttt tggagagcga gacgatcaac 1561 aggtgtttat ccagaaagtt gttcccatca ccaacaaact atttgtaaga ctctcatcga 1621 ctggaaaaag aatatgtgag atccaggctg ttgactgtac tacaatatcc tcatttacag 1681 tgagggaatg tgagggatcc agtaggatgg gctcaagacc aaggcgctac ttgttcacag 1741 gccatacaaa tggcagtatt caaatgtggg atctgaccac tgctatggat atggttaaca 1801 aaagtgaaga taaggatgta ggtggtccaa ccgaagaaga gctactcaaa ttactcgatc 1861 aatgtgattt gagcacatct cgctgtgcta ctcctaacat cagtccagca acttccgtag 1921 ttcagcatag ccacttacga gaatcaaatt ctagccttca gcttcagcac catgatacca 1981 cccatgaagc agctacttac ggttccatga ggccTTACAG AGAAAGTCCT TTATTAGCAA 2041 GGGCAAGAAG GACTGAGAGC TTTCACAGTT ATAGGGACTT CCAGACTATT AATTTGAACA 2101 GAAATGTAGA AAGAGCTGTC CCTGAAAATG GTAACTTGGG TCCAATACAA GCTGAAGTGA 2161 AAGGGGCAAC AGGGGAATGT AATATATCTG AGAGAAAGTC TCCTGGAGTA GAAATAAAAA 2221 GTTTGAGAGA ATTGGATAGT GGATTGGAAG TGCATAAAAT AGCTGAAGGT TTTTCAGAAT 2281 CCAAGAAAAG GTCATCAGAA GATGAAAATG AAAATAAAAT AGAGTTTAGG AAGAAAGGAG 2341 GATTTGAAGG GGGAGGATTC CTTGGAAGAA AGAAAGTTCC CTATCTGGCA TCATCACCAA 2401 GTACTTCCGA TGGAGGAACT GACTCACCTG GTACTGCGTC CCCATCTCCT ACAAAGACTA 2461 CTCCATCTCC TCGGCATAAA AAAAGTGATT CTTCAGGTCA GGAGTACAGC TTGTTGCCAA 2521 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2581 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2641 TATCTTGTGG AAAGGACGAG AATGTATTCG ATCCTGATCA GAAACGCGTT AAGTCgacaa 2701 tcaacctctg gattacaaaa tttgtgaaag att