Construct: ORF ccsbBroad304_00076
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002741.1_s304c1, BRDN0000409010
- Derived from:
- ccsbBroadEn_00076
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APBB1 (322)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00076
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 322 | APBB1 | amyloid beta precursor prot... | NM_145689.3 | 100% | 100% | |
| 2 | human | 322 | APBB1 | amyloid beta precursor prot... | NM_001164.5 | 99.7% | 99.7% | 1381_1386delAGAGAG |
| 3 | human | 322 | APBB1 | amyloid beta precursor prot... | NR_047512.2 | 87.4% | (many diffs) | |
| 4 | human | 322 | APBB1 | amyloid beta precursor prot... | NM_001257323.2 | 68% | 66.9% | (many diffs) |
| 5 | human | 322 | APBB1 | amyloid beta precursor prot... | NM_001257319.2 | 67.8% | 66.7% | (many diffs) |
| 6 | human | 322 | APBB1 | amyloid beta precursor prot... | NM_001257325.2 | 66% | 65.9% | (many diffs) |
| 7 | human | 322 | APBB1 | amyloid beta precursor prot... | NM_001257320.2 | 63.2% | 63.2% | 0_1ins777;604_609delAGAGAG |
| 8 | human | 322 | APBB1 | amyloid beta precursor prot... | NM_001257321.2 | 63.2% | 63.2% | 0_1ins777;604_609delAGAGAG |
| 9 | human | 322 | APBB1 | amyloid beta precursor prot... | NM_001257326.2 | 63.2% | 63.2% | 0_1ins777;604_609delAGAGAG |
| 10 | mouse | 11785 | Apbb1 | amyloid beta (A4) precursor... | NM_001253886.1 | 90.4% | 94.6% | (many diffs) |
| 11 | mouse | 11785 | Apbb1 | amyloid beta (A4) precursor... | NM_009685.3 | 90.4% | 94.6% | (many diffs) |
| 12 | mouse | 11785 | Apbb1 | amyloid beta (A4) precursor... | XM_017321946.1 | 90.4% | 94.6% | (many diffs) |
| 13 | mouse | 11785 | Apbb1 | amyloid beta (A4) precursor... | NM_001253885.1 | 90.1% | 94.3% | (many diffs) |
| 14 | mouse | 11785 | Apbb1 | amyloid beta (A4) precursor... | XM_006507230.2 | 90.1% | 94.3% | (many diffs) |
| 15 | mouse | 11785 | Apbb1 | amyloid beta (A4) precursor... | XM_006507231.1 | 90.1% | 94.3% | (many diffs) |
| 16 | mouse | 11785 | Apbb1 | amyloid beta (A4) precursor... | XM_006507232.3 | 64.1% | 64.5% | (many diffs) |
| 17 | mouse | 11785 | Apbb1 | amyloid beta (A4) precursor... | NM_001310600.1 | 61.7% | 64.2% | (many diffs) |
| 18 | mouse | 11785 | Apbb1 | amyloid beta (A4) precursor... | XM_017321947.1 | 61.6% | 64% | (many diffs) |
| 19 | mouse | 11785 | Apbb1 | amyloid beta (A4) precursor... | NM_001253887.1 | 57.9% | 60.7% | (many diffs) |
| 20 | mouse | 11785 | Apbb1 | amyloid beta (A4) precursor... | XM_006507234.3 | 57.9% | 60.7% | (many diffs) |
| 21 | mouse | 11785 | Apbb1 | amyloid beta (A4) precursor... | XM_006507235.3 | 57.9% | 60.7% | (many diffs) |
| 22 | mouse | 11785 | Apbb1 | amyloid beta (A4) precursor... | XM_006507236.3 | 57.9% | 60.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2190
- ORF length:
- 2124
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgttccatca tcactgagcc agtcggccat taatgccaac agccacggag 121 gccccgcact gagcctaccc ctgcctctgc acgctgccca caaccagctg ctcaacgcca 181 agctgcaggc cacagctgtg ggacccaagg acctgcgcag cgccatgggg gagggtggtg 241 ggcctgagcc aggccctgcc aatgccaagt ggctaaaaga gggccagaac cagctccggc 301 gggccgccac ggcccaccgt gaccagaatc gcaatgtgac cttgaccttg gcggaggagg 361 ccagccagga gcctgagatg gcacccttgg gccccaaagg cctgatacac ctgtactctg 421 agctggagct ctcagctcac aacgcagcca accgaggcct acgaggacct ggcctgatca 481 tcagcactca agagcagggg ccagatgagg gagaggagaa ggcggccggg gaggccgagg 541 aggaggagga ggatgatgat gatgaagagg aggaggagga cttatcttct cccccagggc 601 tgcctgagcc cctggagagt gtggaggccc ctcccaggcc ccaagccctt acagatggcc 661 cccgggaaca cagcaagagt gccagcctcc tgtttggcat gcggaacagt gcagccagtg 721 atgaggactc aagctgggct accttatccc agggcagccc ctcctatggc tccccagagg 781 acacagattc cttctggaac cccaacgcct tcgagacgga ttccgacctg ccggctggat 841 ggatgagggt ccaggacacc tcagggacct attactggca catcccaaca gggaccaccc 901 agtgggaacc ccccggccgg gcctccccct cacaggggag cagcccccaa gaggagtccc 961 agctcacctg gacaggtttt gctcatggag aaggctttga ggatggagaa ttttggaagg 1021 atgaacccag tgatgaggcc ccaatggagc tgggactgaa ggaacctgag gaggggacgt 1081 tgaccttccc agctcagagc ctcagcccag agccgttgcc ccaagaggag gagaagcttc 1141 ccccacggaa taccaaccca gggatcaagt gtttcgccgt gcgctcccta ggctgggtag 1201 agatgaccga ggaggagctg gcccctggac gcagcagtgt ggcagtcaac aattgcatcc 1261 gtcagctctc ttaccacaaa aacaacctgc atgaccccat gtctgggggc tggggggaag 1321 gaaaggatct gctactgcag ctggaggatg agacactaaa gctagtggag ccacagagcc 1381 aggcactgct gcacgcccaa cccatcatca gcatccgcgt gtggggcgtc gggcgggaca 1441 gtggaaggga ctttgcctac gtagctcgtg ataagctgac ccagatgctc aagtgccacg 1501 tgtttcgctg tgaggcacct gccaagaaca tcgccaccag cctgcatgag atctgctcta 1561 agatcatggc cgaacggcgt aatgcccgct gcttggtaaa tggactctcc ctggaccact 1621 ctaaacttgt ggatgtccct ttccaagtgg aattcccagc gcctaagaat gagttggtcc 1681 agaagttcca agtctattac ctggggaatg tacctgttgc taaacctgtt ggggtagatg 1741 tgattaatgg ggccctcgag tcagtcctgt cctccagcag ccgtgaacaa tggaccccaa 1801 gtcatgtcag tgtggcccct gctaccctca ccatcttgca ccagcagaca gaggcagtgc 1861 tgggagagtg tcgggtgcgt ttcctctcct tcctggccgt gggcagagat gtccacacgt 1921 ttgcattcat catggctgcc ggcccagcct ccttctgctg ccacatgttc tggtgcgagc 1981 ccaatgctgc cagcctctca gaggctgtgc aggctgcgtg catgcttcgc taccagaagt 2041 gtctggatgc ccgttcccag gcctccacct cctgcctccc agcaccccct gctgagtctg 2101 tggcacggcg tgtagggtgg actgtccgca ggggtgttca gtcgctgtgg ggctccctga 2161 agcccaaacg gctgggggcc cataccccat gcccaacttt cttgtacaaa gtggttggta 2221 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 2281 ggtggcgcgt taagtcgaca atcaacctct ggatta