Construct: ORF ccsbBroad304_00206
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005470.1_s304c1, BRDN0000400264
- Derived from:
- ccsbBroadEn_00206
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CALD1 (800)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00206
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 800 | CALD1 | caldesmon 1 | NM_004342.7 | 100% | 100% | |
| 2 | human | 800 | CALD1 | caldesmon 1 | XM_017012652.1 | 100% | 100% | |
| 3 | human | 800 | CALD1 | caldesmon 1 | XM_024446943.1 | 99.8% | 99.8% | 1212_1213insAGC |
| 4 | human | 800 | CALD1 | caldesmon 1 | NM_033140.4 | 96.5% | 95.5% | (many diffs) |
| 5 | human | 800 | CALD1 | caldesmon 1 | XM_017012654.2 | 96.3% | 95.3% | (many diffs) |
| 6 | human | 800 | CALD1 | caldesmon 1 | XM_024446942.1 | 95.3% | 95.3% | 620_697del |
| 7 | human | 800 | CALD1 | caldesmon 1 | NM_033157.4 | 95.2% | 95.2% | 620_697del;1290_1291insAGC |
| 8 | human | 800 | CALD1 | caldesmon 1 | NM_033139.3 | 92.1% | 91.1% | (many diffs) |
| 9 | human | 800 | CALD1 | caldesmon 1 | NM_033138.4 | 67.8% | 67.8% | 620_1384del |
| 10 | human | 800 | CALD1 | caldesmon 1 | XM_017012650.2 | 67.7% | 67.7% | 620_1384del;1977_1978insAGC |
| 11 | human | 800 | CALD1 | caldesmon 1 | XR_927541.2 | 37.1% | 1_240del;1771_1885del;1970_4346del | |
| 12 | human | 800 | CALD1 | caldesmon 1 | XR_002956490.1 | 37% | 1_250del;1781_1895del;1980_4356del | |
| 13 | human | 800 | CALD1 | caldesmon 1 | XR_001744880.2 | 36.9% | (many diffs) | |
| 14 | human | 800 | CALD1 | caldesmon 1 | XR_927542.3 | 36.6% | (many diffs) | |
| 15 | human | 800 | CALD1 | caldesmon 1 | XR_001744881.2 | 36.5% | (many diffs) | |
| 16 | human | 800 | CALD1 | caldesmon 1 | XR_002956489.1 | 36.4% | (many diffs) | |
| 17 | human | 800 | CALD1 | caldesmon 1 | XR_001744879.2 | 35.9% | (many diffs) | |
| 18 | human | 800 | CALD1 | caldesmon 1 | XR_927535.2 | 31.5% | (many diffs) | |
| 19 | human | 800 | CALD1 | caldesmon 1 | XR_002956488.1 | 31.5% | (many diffs) | |
| 20 | human | 800 | CALD1 | caldesmon 1 | XR_001744877.2 | 31.4% | (many diffs) | |
| 21 | human | 800 | CALD1 | caldesmon 1 | XR_927537.3 | 31% | (many diffs) | |
| 22 | mouse | 109624 | Cald1 | caldesmon 1 | NM_001347100.1 | 85% | 89.4% | (many diffs) |
| 23 | mouse | 109624 | Cald1 | caldesmon 1 | XM_006505352.2 | 84.9% | 89% | (many diffs) |
| 24 | mouse | 109624 | Cald1 | caldesmon 1 | NM_145575.3 | 81.9% | 85.2% | (many diffs) |
| 25 | mouse | 109624 | Cald1 | caldesmon 1 | XM_006505351.2 | 81.8% | 84.8% | (many diffs) |
| 26 | mouse | 109624 | Cald1 | caldesmon 1 | XM_017321320.1 | 79.1% | 84.3% | (many diffs) |
| 27 | mouse | 109624 | Cald1 | caldesmon 1 | XM_006505348.3 | 60% | 62% | (many diffs) |
| 28 | mouse | 109624 | Cald1 | caldesmon 1 | XM_006505346.2 | 58.1% | 59.3% | (many diffs) |
| 29 | mouse | 109624 | Cald1 | caldesmon 1 | XM_006505345.3 | 58% | 59.1% | (many diffs) |
| 30 | mouse | 109624 | Cald1 | caldesmon 1 | XM_017321316.1 | 56.6% | 59% | (many diffs) |
| 31 | mouse | 109624 | Cald1 | caldesmon 1 | XM_017321317.1 | 56.6% | 59% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1680
- ORF length:
- 1614
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga tgattttgag cgtcgcagag aacttagaag gcaaaagagg gaggagatgc 121 gactcgaagc agaaagaatc gcctaccaga ggaatgacga tgatgaagag gaggcagccc 181 gggaacggcg ccgccgagcc cgacaggaac ggctgcggca gaagcaggag gaagaatcct 241 tgggacaggt gaccgaccag gtggaggtga atgcccagaa cagtgtgcct gacgaggagg 301 ccaagacaac caccacaaac actcaagtgg aaggggatga tgaggccgca ttcctggagc 361 gcctggctcg gcgtgaggaa agacgccaaa aacgccttca ggaggctctg gagcggcaga 421 aggagttcga cccaacaata acagatgcaa gtctgtcgct cccaagcaga agaatgcaaa 481 atgacacagc agaaaatgaa actaccgaga aggaagaaaa aagtgaaagt cgccaagaaa 541 gatacgagat agaggaaaca gaaacagtca ccaagtccta ccagaagaat gattggaggg 601 atgctgaaga aaacaagaaa gaagacaagg aaaaggagga ggaggaagag gagaagccaa 661 agcgagggag cattggagaa aatcagatca aagatgaaaa gattaaaaag gacaaagaac 721 ccaaagaaga agttaagagc ttcatggatc gaaagaaggg atttacagaa gttaagtcgc 781 agaatggaga attcatgacc cacaaactta aacatactga gaatactttc agccgccctg 841 gagggagggc cagcgtggac accaaggagg ctgagggcgc cccccaggtg gaagccggca 901 aaaggctgga ggagcttcgt cgtcgtcgcg gggagaccga gagcgaagag ttcgagaagc 961 tcaaacagaa gcagcaggag gcggctttgg agctggagga actcaagaaa aagagggagg 1021 agagaaggaa ggtcctggag gaggaagagc agaggaggaa gcaggaggaa gccgatcgaa 1081 aactcagaga ggaggaagag aagaggaggc taaaggaaga gattgaaagg cgaagagcag 1141 aagctgctga gaaacgccag aagatgccag aagatggctt gtcagatgac aagaaaccat 1201 tcaagtgttt cactcctaaa ggttcatctc tcaagataga agagcgagca gaatttttga 1261 ataagtctgt gcagaaaagc agtggtgtca aatcgaccca tcaagcagca atagtctcca 1321 agattgacag cagactggag cagtatacca gtgcaattga gggaacaaaa agcgcaaaac 1381 ctacaaagcc ggcagcctcg gatcttcctg ttcctgctga aggtgtacgc aacatcaaga 1441 gtatgtggga gaaagggaat gtgttttcat cccccactgc agcaggcaca ccaaataagg 1501 aaactgctgg cttgaaggta ggggtttcta gccgcatcaa tgaatggcta actaaaaccc 1561 cagatggaaa caagtcacct gctcccaaac cttctgactt gagaccagga gacgtatcca 1621 gcaagcggaa cctctgggaa aagcaatctg tggataaggt cacttccccc actaaggttt 1681 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1741 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1801 ggatta