Construct: ORF ccsbBroad304_00343
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009340.1_s304c1, BRDN0000405253
- Derived from:
- ccsbBroadEn_00343
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COL10A1 (1300)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00343
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1300 | COL10A1 | collagen type X alpha 1 chain | NM_000493.4 | 100% | 100% | |
| 2 | human | 1300 | COL10A1 | collagen type X alpha 1 chain | XM_006715333.3 | 100% | 100% | |
| 3 | human | 1300 | COL10A1 | collagen type X alpha 1 chain | XM_011535432.3 | 100% | 100% | |
| 4 | human | 1300 | COL10A1 | collagen type X alpha 1 chain | XM_011535433.3 | 100% | 100% | |
| 5 | human | 1300 | COL10A1 | collagen type X alpha 1 chain | XM_017010248.1 | 100% | 100% | |
| 6 | mouse | 12813 | Col10a1 | collagen, type X, alpha 1 | NM_009925.4 | 85.7% | 87.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2109
- ORF length:
- 2040
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgccacaa ataccctttt tgctgctagt atccttgaac ttggttcatg 121 gagtgtttta cgctgaacga taccaaatgc ccacaggcat aaaaggccca ctacccaaca 181 ccaagacaca gttcttcatt ccctacacca taaagagtaa aggtatagca gtaagaggag 241 agcaaggtac tcctggtcca ccaggccctg ctggacctcg agggcaccca ggtccttctg 301 gaccaccagg aaaaccaggc tacggaagtc ctggactcca aggagagcca gggttgccag 361 gaccaccggg accatcagct gtagggaaac caggtgtgcc aggactccca ggaaaaccag 421 gagagagagg accatatgga ccaaaaggag atgttggacc agctggccta ccaggacccc 481 ggggcccacc aggaccacct ggaatccctg gaccggctgg aatttctgtg ccaggaaaac 541 ctggacaaca gggacccaca ggagccccag gacccagggg ctttcctgga gaaaagggtg 601 caccaggagt ccctggtatg aatggacaga aaggggaaat gggatatggt gctcctggtc 661 gtccaggtga gaggggtctt ccaggccctc agggtcccac aggaccatct ggccctcctg 721 gagtgggaaa aagaggtgaa aatggggttc caggacagcc aggcatcaaa ggtgatagag 781 gttttccggg agaaatggga ccaattggcc caccaggtcc ccaaggccct cctggggaac 841 gagggccaga aggcattgga aagccaggag ctgctggagc cccaggccag ccagggattc 901 caggaacaaa aggtctccct ggggctccag gaatagctgg gcccccaggg cctcctggct 961 ttgggaaacc aggcttgcca ggcctgaagg gagaaagagg acctgctggc cttcctgggg 1021 gtccaggtgc caaaggggaa caagggccag caggtcttcc tgggaagcca ggtctgactg 1081 gaccccctgg gaatatggga ccccaaggac caaaaggcat cccgggtagc catggtctcc 1141 caggccctaa aggtgagaca gggccagctg ggcctgcagg ataccctggg gctaagggtg 1201 aaaggggttc ccctgggtca gatggaaaac cagggtaccc aggaaaacca ggtctcgatg 1261 gtcctaaggg taacccaggg ttaccaggtc caaaaggtga tcctggagtt ggaggacctc 1321 ctggtctccc aggccctgtg ggcccagcag gagcaaaggg aatgcccgga cacaatggag 1381 aggctggccc aagaggtgcc cctggaatac caggtactag aggccctatt gggccaccag 1441 gcattccagg attccctggg tctaaagggg atccaggaag tcccggtcct cctggcccag 1501 ctggcatagc aactaagggc ctcaatggac ccaccgggcc accagggcct ccaggtccaa 1561 gaggccactc tggagagcct ggtcttccag ggccccctgg gcctccaggc ccaccaggtc 1621 aagcagtcat gcctgagggt tttataaagg caggccaaag gcccagtctt tctgggaccc 1681 ctcttgttag tgccaaccag ggggtaacag gaatgcctgt gtctgctttt actgttattc 1741 tctccaaagc ttacccagca ataggaactc ccataccatt tgataaaatt ttgtataaca 1801 ggcaacagca ttatgaccca aggactggaa tctttacttg tcagatacca ggaatatact 1861 atttttcata ccacgtgcat gtgaaaggga ctcatgtttg ggtaggcctg tataagaatg 1921 gcacccctgt aatgtacacc tatgatgaat acaccaaagg ctacctggat caggcttcag 1981 ggagtgccat catcgatctc acagaaaatg accaggtgtg gctccagctt cccaatgccg 2041 agtcaaatgg cctatactcc tctgagtatg tccactcctc tttctcagga ttcctagtgg 2101 ctccaatgtt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 2161 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 2221 tcaacctctg gatta