Construct: ORF ccsbBroad304_00421
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013692.1_s304c1, BRDN0000401879
- Derived from:
- ccsbBroadEn_00421
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DAPK1 (1612)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00421
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1612 | DAPK1 | death associated protein ki... | NM_001288729.1 | 100% | 100% | |
| 2 | human | 1612 | DAPK1 | death associated protein ki... | NM_001288730.2 | 100% | 100% | |
| 3 | human | 1612 | DAPK1 | death associated protein ki... | NM_001288731.2 | 100% | 100% | |
| 4 | human | 1612 | DAPK1 | death associated protein ki... | NM_004938.4 | 100% | 100% | |
| 5 | human | 1612 | DAPK1 | death associated protein ki... | XM_017014360.1 | 70% | 69.3% | (many diffs) |
| 6 | human | 1612 | DAPK1 | death associated protein ki... | XM_005251757.4 | 57.8% | 56.8% | (many diffs) |
| 7 | mouse | 69635 | Dapk1 | death associated protein ki... | NM_029653.3 | 87.6% | 95.1% | (many diffs) |
| 8 | mouse | 69635 | Dapk1 | death associated protein ki... | NM_134062.2 | 87.6% | 95.1% | (many diffs) |
| 9 | mouse | 69635 | Dapk1 | death associated protein ki... | NM_001285917.1 | 86.9% | 94.3% | (many diffs) |
| 10 | mouse | 69635 | Dapk1 | death associated protein ki... | XM_006517370.3 | 86.9% | 94.3% | (many diffs) |
| 11 | mouse | 69635 | Dapk1 | death associated protein ki... | XM_006517371.3 | 86.4% | 93.7% | (many diffs) |
| 12 | mouse | 69635 | Dapk1 | death associated protein ki... | XM_017315598.1 | 85.6% | 92.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4359
- ORF length:
- 4290
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccgtgttc aggcaggaaa acgtggatga ttactacgac accggcgagg 121 aacttggcag tggacagttt gcggttgtga agaaatgccg tgagaaaagc accggcctcc 181 agtatgccgc caaattcatc aagaaaagga ggactaagtc cagccggcgg ggtgtgagcc 241 gcgaggacat cgagcgggag gtcagcatcc tgaaggagat ccagcacccc aatgtcatca 301 ccctgcacga ggtctatgag aacaagacgg acgtcatcct gatcttggaa ctcgttgcag 361 gtggcgagct gtttgacttc ttagctgaaa aggaatcttt aactgaagag gaagcaactg 421 aatttctcaa acaaattctt aatggtgttt actacctgca ctcccttcaa atcgcccact 481 ttgatcttaa gcctgagaac ataatgcttt tggatagaaa tgtccccaaa cctcggatca 541 agatcattga ctttgggttg gcccataaaa ttgactttgg aaatgaattt aaaaacatat 601 ttgggactcc agagtttgtc gctcctgaga tagtcaacta tgaacctctt ggtcttgagg 661 cagatatgtg gagtatcggg gtaataacct atatcctcct aagtggggcc tccccatttc 721 ttggagacac taagcaagaa acgttagcaa atgtatccgc tgtcaactac gaatttgagg 781 atgaatactt cagtaatacc agtgccctag ccaaagattt cataagaaga cttctggtca 841 aggatccaaa gaagagaatg acaattcaag atagtttgca gcatccctgg atcaagccta 901 aagatacaca acaggcactt agtagaaaag catcagcagt aaacatggag aaattcaaga 961 agtttgcagc ccggaaaaaa tggaaacaat ccgttcgctt gatatcactg tgccaaagat 1021 tatccaggtc attcctgtcc agaagtaaca tgagtgttgc cagaagcgat gatactctgg 1081 atgaggaaga ctcctttgtg atgaaagcca tcatccatgc catcaacgat gacaatgtcc 1141 caggcctgca gcaccttctg ggctcattat ccaactatga tgttaaccaa cccaacaagc 1201 acgggacacc tccattactc attgctgctg gctgtgggaa tattcaaata ctacagttgc 1261 tcattaaaag aggctcgaga atcgatgtcc aggataaggg cgggtccaat gccgtctact 1321 gggctgctcg gcatggccac gtcgatacct tgaaatttct cagtgagaac aaatgccctt 1381 tggatgtgaa agacaagtct ggagagatgg ccctccacgt ggcagctcgc tatggccatg 1441 ctgacgtggc tcagttactg tgcagcttcg gctcaaatcc caatatccag gacaaggaag 1501 aagaaacccc cctgcactgt gctgcttggc acggctatta ctctgtggcc aaagcccttt 1561 gtgaagccgg ctgtaacgtg aacatcaaga accgagaagg agagacgccc ctcctgacag 1621 cctctgccag gggctaccac gacatcgtgg agtgtctggc cgaacatgga gccgacctta 1681 atgcttgcga caaggacgga cacattgccc ttcatctggc tgtaagacgg tgtcagatgg 1741 aggtaatcaa gactctcctc agccaagggt gtttcgtcga ttatcaagac aggcacggca 1801 atactcccct ccatgtggca tgtaaagatg gcaacatgcc tatcgtggtg gccctctgtg 1861 aagcaaactg caatttggac atctccaaca agtatgggcg aacgcctctg caccttgcgg 1921 ccaacaacgg aatcctagac gtggtccggt atctctgtct gatgggagcc agcgttgagg 1981 cgctgaccac ggacggaaag acggcagaag atcttgctag atcggaacag cacgagcacg 2041 tagcaggtct ccttgcaaga cttcgaaagg atacgcaccg aggactcttc atccagcagc 2101 tccgacccac acagaacctg cagccaagaa ttaagctcaa gctgtttggc cactcgggat 2161 ccgggaaaac cacccttgta gaatctctca agtgtgggct gctgaggagc tttttcagaa 2221 ggcgtcggcc cagactgtct tccaccaact ccagcaggtt cccaccttca cccctggctt 2281 ctaagcccac agtctcagtg agcatcaaca acctgtaccc aggctgcgag aacgtgagtg 2341 tgaggagccg cagcatgatg ttcgagccgg gtcttaccaa agggatgctg gaggtgtttg 2401 tggccccgac ccaccacccg cactgctcgg ccgatgacca gtccaccaag gccatcgaca 2461 tccagaacgc ttatttgaat ggagttggcg atttcagcgt gtgggagttc tctggaaatc 2521 ctgtgtattt ctgctgttat gactattttg ctgcaaatga tcccacgtca atccatgttg 2581 ttgtctttag tctagaagag ccctatgaga tccagctgaa ccaagtgatt ttctggctca 2641 gtttcctgaa gtcccttgtc ccagttgaag aacccatagc cttcggtggc aagctgaaga 2701 acccactcca agttgtcctg gtggccaccc acgctgacat catgaatgtt cctcgaccgg 2761 ctggaggcga gtttggatat gacaaagaca catcgttgct gaaagagatt aggaacaggt 2821 ttggaaatga tcttcacatt tcaaataagc tgtttgttct ggatgctggg gcttctgggt 2881 caaaggacat gaaggtactt cgaaatcatc tgcaagaaat acgaagccag attgtttcgg 2941 tctgtcctcc catgactcac ctgtgtgaga aaatcatctc cacgctgcct tcctggagga 3001 agctcaatgg acccaaccag ctgatgtcgc tgcagcagtt tgtgtacgac gtgcaggacc 3061 agctgaaccc cctggccagc gaggaggacc tcaggcgcat tgctcagcag ctccacagca 3121 caggcgagat caacatcatg caaagtgaaa cagttcagga cgtgctgctc ctggaccccc 3181 gctggctctg cacaaacgtc ctggggaagt tgctgtccgt ggagacccca cgggcgctgc 3241 accactaccg gggccgctac accgtggagg acatccagcg cctggtgccc gacagcgacg 3301 tggaggagct gctgcagatc ctcgatgcca tggacatctg cgcccgggac ctgagcagcg 3361 ggaccatggt ggacgtccca gccctgatca agacagacaa cctgcaccgc tcctgggctg 3421 atgaggagga cgaggtgatg gtgtatggtg gcgtgcgcat cgtgcccgtg gaacacctca 3481 cccccttccc atgtggcatc tttcacaagg tccaggtgaa cctgtgccgg tggatccacc 3541 agcaaagcac agagggcgac gcggacatcc gcctgtgggt gaatggctgc aagctggcca 3601 accgtggggc cgagctgctg gtgctgctgg tcaaccacgg ccagggcatt gaggtccagg 3661 tccgcggcct ggagacggag aagatcaagt gctgcctgct gctggactcg gtgtgcagca 3721 ccattgagaa cgtcatggcc accacgctgc cagggctcct gaccgtgaag cattacctga 3781 gcccccagca gctgcgggag caccatgagc ccgtcatgat ctaccagcca cgggacttct 3841 tccgggcaca gactctgaag gaaacctcac tgaccaacac catggggggg tacaaggaaa 3901 gcttcagcag catcatgtgc ttcgggtgtc acgacgtcta ctcacaggcc agcctcggca 3961 tggacatcca tgcatcagac ctgaacctcc tcactcggag gaaactgagt cgcctgctgg 4021 acccgcccga ccccctgggg aaggactggt gccttctcgc catgaactta ggcctccctg 4081 acctcgtggc aaagtacaac accagtaacg gggctcccaa ggatttcctc cccagccccc 4141 tccacgccct gctgcgggaa tggaccacct accctgagag cacagtgggc accctcatgt 4201 ccaaactgag ggagctgggt cgccgggatg ccgcagactt tttgctgaag gcatcctctg 4261 tgttcaaaat caacctggat ggcaatggcc aggaggccta tgcctcgagc tgcaacagcg 4321 gcacctctta caattccatt agctctgttg tatcccggtt gccaactttc ttgtacaaag 4381 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 4441 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta