Construct: ORF ccsbBroad304_00448
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010275.1_s304c1, BRDN0000462128
- Derived from:
- ccsbBroadEn_00448
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DLG3 (1741)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00448
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | NM_021120.4 | 100% | 100% | |
| 2 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_006724626.2 | 98.3% | 98.3% | 1819_1860del |
| 3 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_011530883.1 | 97.2% | 95.9% | (many diffs) |
| 4 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_006724625.2 | 95.6% | 94.3% | (many diffs) |
| 5 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_017029324.2 | 56.9% | 54.3% | (many diffs) |
| 6 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_017029323.2 | 56% | 53.4% | (many diffs) |
| 7 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_017029322.2 | 55.1% | 51.5% | (many diffs) |
| 8 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | NM_020730.3 | 54.3% | 50.7% | (many diffs) |
| 9 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_017029327.1 | 40.8% | 40.8% | 0_1ins1449 |
| 10 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_017029325.2 | 40.6% | 39.5% | (many diffs) |
| 11 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | NM_001166278.1 | 38.8% | 37.8% | (many diffs) |
| 12 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_005262248.4 | 38.8% | 37.8% | (many diffs) |
| 13 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_017029326.2 | 38.8% | 37.8% | (many diffs) |
| 14 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | NM_001290402.1 | 93% | 99.2% | (many diffs) |
| 15 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | NM_001177778.2 | 91% | 97.1% | (many diffs) |
| 16 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | NM_016747.4 | 89.5% | 95.5% | (many diffs) |
| 17 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | XM_006528124.3 | 89% | 93.6% | (many diffs) |
| 18 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | XM_006528123.3 | 87.2% | 91.7% | (many diffs) |
| 19 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | NM_001177779.1 | 51.4% | 50.7% | (many diffs) |
| 20 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | XR_001782754.1 | 42.8% | (many diffs) | |
| 21 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | NM_001177780.1 | 36.5% | 37.7% | (many diffs) |
| 22 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | XM_006528127.3 | 36.5% | 37.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2520
- ORF length:
- 2451
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcgccat gcacaagcac cagcactgct gtaagtgccc tgagtgctat gaggtgaccc 121 gcctggccgc cctgcggcgc ctcgagcctc cgggctacgg cgactggcaa gtccccgacc 181 cttacgggcc aggtgggggc aacggcgcca gcgcgggtta tgggggctac agctcgcaga 241 ccttgccctc gcaggcgggg gccaccccca cccctcgcac caaggccaag ctcatcccca 301 ccggccggga tgtggggccg gtgcctccta agccagtccc gggcaagagc acccccaaac 361 tcaacggcag cggccccagc tggtggccag agtgcacctg taccaaccgg gactggtatg 421 agcaggtgaa tggcagtgat ggcatgttca aatatgagga aatcgtactt gagaggggca 481 actctggcct gggcttcagt atcgcaggtg gcatcgacaa tccccatgtc cctgatgacc 541 ctggcatctt tattaccaag attatccctg gtggagcagc tgccatggat gggaggctgg 601 gggtgaatga ctgtgtgctg cgggtgaatg aggtggacgt gtcggaggtg gtacacagcc 661 gggcggtgga ggcgctgaag gaggcaggcc ctgtggtgcg attggtggtg cggaggcgac 721 agcctccacc cgagaccatc atggaggtca acctgctcaa agggcccaaa ggcctgggtt 781 tcagcattgc tgggggtatt ggcaaccagc acatcccagg agacaacagc atctacatca 841 ccaagatcat tgaggggggt gctgctcaga aggatggacg cctacagatt ggggaccggc 901 tgctggcggt gaacaacacc aatctgcagg atgtgaggca cgaggaagct gtggcctcac 961 tgaagaacac atctgatatg gtgtatttga aggtggccaa gccaggcagc ctccacctca 1021 acgacatgta cgctccccct gactacgcca gcacttttac tgccttggct gacaaccaca 1081 taagccataa ttccagcctg ggttatctcg gggctgtgga gagcaaggtc agctaccctg 1141 ctcctcctca ggttcccccc acccgctact ctcctattcc caggcacatg ctggctgagg 1201 aggacttcac cagagagcct cgcaagatca tcctgcacaa aggctccaca ggcctgggct 1261 tcaacatcgt aggaggagag gatggagaag gcatttttgt ctccttcatc ctggcaggag 1321 gcccagctga cctgagtggg gagctgcgca ggggagaccg gatcttatcg gtgaatggag 1381 tgaatctgag gaatgcaact catgagcagg ctgcagctgc tctgaaacgg gccggccagt 1441 cagtcaccat tgtggcccag tacagacctg aagaatacag tcgctttgaa tcgaagatac 1501 atgacttacg agaacaaatg atgaacagca gcatgagctc tgggtctggg tccctccgaa 1561 caagtgaaaa gaggtccttg tatgtcaggg ccctgtttga ttatgatcgg actcgggaca 1621 gctgcctgcc aagccagggg ctcagcttct cttatggtga cattctgcat gtcattaatg 1681 cctctgatga tgagtggtgg caggcaaggc tggtgacccc acacggagaa agtgagcaga 1741 tcggtgtgat ccccagtaag aagagggtgg aaaagaaaga aagagctcga ttgaaaactg 1801 tgaagttcca tgccaggacg gggatgattg agtctaacag ggacttcccg gggttaagtg 1861 acgattatta tggagcaaag aacctgaaag gacaagagga tgctattttg tcatatgagc 1921 cagtgacacg gcaagaaatt cactatgcaa ggcctgtgat catcctgggc ccaatgaagg 1981 accgagtcaa tgatgacctg atctccgaat ttccacataa atttggatcc tgtgtgccac 2041 atactacccg gcctcgacgt gataatgagg tggatggaca agactaccac tttgtggtgt 2101 cccgagaaca aatggagaaa gatattcagg acaacaagtt catcgaggcg ggccaattta 2161 atgataacct ctatgggacc agcatccagt cagtgcgggc agttgcagag aggggcaagc 2221 actgcatctt agatgtttcc ggcaatgcta tcaagagact gcagcaagca caactttacc 2281 ccattgccat tttcatcaag cccaagtcca ttgaagccct tatggaaatg aaccgaaggc 2341 agacatatga acaagcaaat aagatctatg acaaagccat gaaactggag caggaatttg 2401 gagagtactt tacagccatt gtacagggtg actcactgga agagatttat aacaaaatca 2461 aacaaatcat tgaggaccag tctgggcact acatttgggt cccatcccct gaaaaactct 2521 tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 2581 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 2641 ggatta