Construct: ORF ccsbBroad304_00540
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012391.1_s304c1, BRDN0000410364
- Derived from:
- ccsbBroadEn_00540
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FARSA (2193)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00540
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2193 | FARSA | phenylalanyl-tRNA synthetas... | NM_004461.3 | 100% | 100% | |
| 2 | mouse | 66590 | Farsa | phenylalanyl-tRNA synthetas... | NM_025648.3 | 85.8% | 92.5% | (many diffs) |
| 3 | mouse | 66590 | Farsa | phenylalanyl-tRNA synthetas... | XR_387772.4 | 69.6% | (many diffs) | |
| 4 | mouse | 66590 | Farsa | phenylalanyl-tRNA synthetas... | NR_152751.1 | 67.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1590
- ORF length:
- 1524
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggatggtcag gtggcggaac tgctgctccg gcggctggag gcgtctgatg 121 gcggcctgga cagcgccgag ttggcggctg agctgggcat ggagcaccag gcggtggtgg 181 gcgccgtgaa gagccttcag gcgctgggcg aggtcatcga ggctgaactt cggtccacca 241 agcactggga gcttactgcg gagggcgagg agattgcccg ggagggcagc catgaggccc 301 gtgtgtttcg aagcattccc ccagagggcc tggcccagag cgagcttatg cgactgccca 361 gtggcaaagt gggcttcagc aaggccatgt ccaacaagtg gattcgggtg gacaagagtg 421 cggctgacgg gccccgggtg ttccgagtgg tggacagcat ggaggatgag gtgcagcggc 481 ggctccagct ggtccggggg ggacaggctg agaagctggg ggagaaggag aggagcgagc 541 tgaggaagag gaagctgttg gctgaagtga ctctgaagac ctactgggtg agcaaaggca 601 gtgcctttag taccagcatc tccaagcaag agacagagct gagcccagag atgatctcca 661 gtggctcttg gcgggaccgg cccttcaagc cctacaactt cttggcccac ggtgtcctcc 721 ccgacagcgg ccaccttcac ccgctgctca aggtccgctc ccagttccga cagatcttcc 781 tggagatggg gttcaccgag atgccgactg ataacttcat tgagagctcc ttctggaact 841 ttgacgccct cttccagccc cagcagcacc cagcccgtga ccagcacgac accttcttcc 901 ttcgagatcc agcggaggcc ctgcagctcc caatggacta tgtccagcgg gtcaagcgga 961 cccactctca gggcggctac ggctcacagg ggtacaagta taactggaag ctggacgagg 1021 cccggaaaaa cctactgcga acccacacca catcagccag cgcccgtgcg ctctaccgcc 1081 ttgcccagaa gaagcccttc actccggtca agtacttctc catcgaccgc gtattccgga 1141 atgagaccct ggacgccacg cacctggctg agttccacca gatcgagggc gtggtggcgg 1201 atcatggtct caccttgggc cacctcatgg gcgttctgcg ggagttcttc accaagctgg 1261 gtatcacgca actccgcttc aagccagcct acaacccata cacagagccc agcatggagg 1321 tgttcagcta ccaccaaggc ctgaagaagt gggtggaggt cggaaactcg ggggtcttcc 1381 gtccagagat gctgctgccc atggggcttc ccgagaacgt gtcggtcatt gcctggggcc 1441 tctccctgga gcgcccaacg atgatcaaat atggcatcaa caatatccgg gagctggtgg 1501 gccacaaggt gaacctgcag atggtgtatg acagtcccct gtgccgcctg gatgccgagc 1561 cgaggccccc tcccacacag gaggctgcgt gcccaacttt cttgtacaaa gtggttggta 1621 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 1681 ggtggcgcgt taagtcgaca atcaacctct ggatta