Construct: ORF ccsbBroad304_00719

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011508.1_s304c1, BRDN0000405809
Derived from:
ccsbBroadEn_00719
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
H3-3B (3021)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00719
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3021 H3-3B H3.3 histone B NM_005324.5 100% 100%
2 human 440093 H3-5 H3.5 histone NM_001013699.3 96.5% 95.5% (many diffs)
3 human 8355 H3C8 H3 clustered histone 8 NM_003534.2 80.8% 96.3% (many diffs)
4 human 333932 H3C15 H3 clustered histone 15 NM_001005464.2 80.5% 97% (many diffs)
5 human 126961 H3C14 H3 clustered histone 14 NM_021059.2 80.5% 97% (many diffs)
6 human 653604 H3C13 H3 clustered histone 13 NM_001123375.2 80.3% 97% (many diffs)
7 human 440686 H3-2 H3.2 histone (putative) NM_001355409.3 80.1% 94.1% (many diffs)
8 human 440686 H3-2 H3.2 histone (putative) NM_001372105.1 80.1% 94.1% (many diffs)
9 human 8351 H3C4 H3 clustered histone 4 NM_003530.4 79.8% 96.3% (many diffs)
10 human 8352 H3C3 H3 clustered histone 3 NM_003531.2 79.7% 96.3% (many diffs)
11 human 8356 H3C12 H3 clustered histone 12 NM_003535.2 79.1% 96.3% (many diffs)
12 human 8354 H3C11 H3 clustered histone 11 NM_003533.2 79.1% 96.3% (many diffs)
13 human 8357 H3C10 H3 clustered histone 10 NM_003536.2 79.1% 96.3% (many diffs)
14 human 8290 H3-4 H3.4 histone NM_003493.2 79.1% 93.3% (many diffs)
15 human 3020 H3-3A H3.3 histone A NM_002107.6 78.4% 100% (many diffs)
16 human 8968 H3C7 H3 clustered histone 7 NM_021018.2 78% 96.3% (many diffs)
17 mouse 15081 H3f3b H3 histone, family 3B NM_008211.3 91.9% 100% (many diffs)
18 mouse 15081 H3f3b H3 histone, family 3B XM_006532248.2 91.9% 100% (many diffs)
19 mouse 97114 Hist2h3c2 histone cluster 2, H3c2 NM_054045.4 83% 97% (many diffs)
20 mouse 15077 Hist2h3c1 histone cluster 2, H3c1 NM_178216.3 83% 97% (many diffs)
21 mouse 319148 Hist1h3c histone cluster 1, H3c NM_175653.2 82.8% 97% (many diffs)
22 mouse 319150 Hist1h3b histone cluster 1, H3b NM_178203.2 82.8% 97% (many diffs)
23 mouse 319149 Hist1h3d histone cluster 1, H3d NM_178204.2 82.8% 97% (many diffs)
24 mouse 319154 Hist2h3b histone cluster 2, H3b NM_178215.2 82.8% 97% (many diffs)
25 mouse 319152 Hist1h3h histone cluster 1, H3h NM_178206.2 82.5% 96.3% (many diffs)
26 mouse 260423 Hist1h3f histone cluster 1, H3f NM_013548.4 82.3% 97% (many diffs)
27 mouse 319151 Hist1h3e histone cluster 1, H3e NM_178205.2 82.3% 97% (many diffs)
28 mouse 360198 Hist1h3a histone cluster 1, H3a NM_013550.5 82.1% 96.3% (many diffs)
29 mouse 97908 Hist1h3g histone cluster 1, H3g NM_145073.2 81.8% 96.3% (many diffs)
30 mouse 319153 Hist1h3i histone cluster 1, H3i NM_178207.2 81.8% 96.3% (many diffs)
31 mouse 382523 Gm12260 predicted gene 12260 NM_001318003.2 78.8% 94.1% (many diffs)
32 mouse 624251 Gm6485 predicted gene 6485 XR_375938.3 39.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
474
ORF length:
408
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ccgaaccaag cagactgctc gtaagtccac cggtgggaaa gccccccgca
121aacagctggc cacgaaagcc gccaggaaaa gcgctccctc taccggcggg gtgaagaagc
181ctcatcgcta caggcccggg accgtggcgc ttcgagagat tcgtcgttat cagaagtcga
241ccgagctgct catccggaag ctgcccttcc agaggttggt gagggagatc gcgcaggatt
301tcaaaaccga cctgaggttt cagagcgcag ccatcggtgc gctgcaggag gctagcgaag
361cgtacctggt gggtctgttc gaagatacca acctgtgtgc catccacgct aagagagtca
421ccatcatgcc caaagacatc cagttggctc gccggatacg gggagagaga gcttacccaa
481ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
541cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)