Construct: ORF ccsbBroad304_00808
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011415.1_s304c1, BRDN0000461316
- Derived from:
- ccsbBroadEn_00808
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HTR7 (3363)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00808
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3363 | HTR7 | 5-hydroxytryptamine receptor 7 | NM_000872.5 | 100% | 100% | |
| 2 | human | 3363 | HTR7 | 5-hydroxytryptamine receptor 7 | NM_019860.4 | 97% | 97% | 1296_1296delGins40 |
| 3 | human | 3363 | HTR7 | 5-hydroxytryptamine receptor 7 | NM_019859.4 | 92.9% | 89.8% | 1296_1393del;1434_1437delTGAT |
| 4 | human | 3363 | HTR7 | 5-hydroxytryptamine receptor 7 | XM_024447973.1 | 51.5% | 48.7% | 0_1ins594;702_799del;840_843delTGAT |
| 5 | human | 93164 | HTR7P1 | 5-hydroxytryptamine recepto... | NR_002774.3 | 27.1% | (many diffs) | |
| 6 | mouse | 15566 | Htr7 | 5-hydroxytryptamine (seroto... | NM_008315.2 | 90.5% | 94.6% | (many diffs) |
| 7 | mouse | 15566 | Htr7 | 5-hydroxytryptamine (seroto... | XM_006526702.3 | 88% | 92.6% | (many diffs) |
| 8 | mouse | 15566 | Htr7 | 5-hydroxytryptamine (seroto... | NM_001347442.1 | 85.2% | 88.5% | (many diffs) |
| 9 | mouse | 15566 | Htr7 | 5-hydroxytryptamine (seroto... | XM_017318067.1 | 49.9% | 53% | (many diffs) |
| 10 | mouse | 15566 | Htr7 | 5-hydroxytryptamine (seroto... | XM_006526704.3 | 46.7% | 48.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1401
- ORF length:
- 1335
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat ggacgttaac agcagcggcc gcccggacct ctacgggcac ctccgctctt 121 tccttctgcc agaagtgggg cgcgggctgc ccgacttgag ccccgacggt ggcgccgacc 181 cggtcgcggg ctcctgggcg ccgcacctgc tgagcgaggt gacagccagc ccggcgccca 241 cctgggacgc gcccccggac aatgcctccg gctgtgggga acagatcaac tacggcagag 301 tcgagaaagt tgtgatcggc tccatcctga cgctcatcac gctgctgacg atcgcgggca 361 actgcctggt ggtgatctcc gtgtgcttcg tcaagaagct ccgccagccc tccaactacc 421 tgatcgtgtc cctggcgctg gccgacctct cggtggctgt ggcggtcatg cccttcgtca 481 gcgtcaccga cctcatcggg ggcaagtgga tctttggaca ctttttctgt aatgtcttca 541 tcgccatgga cgtcatgtgc tgcacggcct cgatcatgac cctgtgcgtg atcagcattg 601 acaggtacct tgggatcaca aggcccctca cataccctgt gaggcagaat gggaaatgca 661 tggcgaagat gattctctcc gtctggcttc tctccgcctc catcacctta cctccactct 721 ttggatgggc tcagaatgta aatgatgata aggtgtgctt gatcagccag gactttggct 781 atacgattta ctctaccgca gtggcatttt atatccccat gtccgtcatg cttttcatgt 841 actaccagat ttacaaggct gccaggaaga gtgctgccaa acacaagttt cctggcttcc 901 ctcgagtgga gccagacagc gtcatcgccc tgaatggcat agtgaagctc cagaaggagg 961 tggaagagtg tgcaaacctt tcgagactcc tcaagcatga aaggaaaaac atctccatct 1021 ttaagcgaga acagaaagca gccaccaccc tggggatcat cgtcggggcc tttaccgtgt 1081 gctggctgcc atttttcctc ctctcgacag ccagaccctt catctgtggc acttcctgca 1141 gctgcatccc actgtgggtg gagaggacat ttctgtggct aggctatgca aactctctca 1201 ttaacccttt tatatatgcc ttcttcaacc gggacctgag gaccacctat cgcagcctgc 1261 tccagtgcca gtaccggaat atcaaccgga agctctcagc tgcaggcatg catgaagccc 1321 tgaagcttgc tgagaggcca gagagacctg agtttgtgct acaaaatgct gactactgta 1381 gaaaaaaagg tcatgattca tgcccaactt tcttgtacaa agtggttggt aagcctatcc 1441 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1501 ttaagtcgac aatcaacctc tggatta