Construct: ORF ccsbBroad304_00961

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002401.1_s304c1, BRDN0000407581
Derived from:
ccsbBroadEn_00961
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SMAD2 (4087)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00961
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4087 SMAD2 SMAD family member 2 NM_001003652.4 100% 100%
2 human 4087 SMAD2 SMAD family member 2 NM_005901.6 100% 100%
3 human 4087 SMAD2 SMAD family member 2 XM_005258259.4 100% 100%
4 human 4087 SMAD2 SMAD family member 2 XM_006722451.4 100% 100%
5 human 4087 SMAD2 SMAD family member 2 XM_017025745.2 100% 100%
6 human 4087 SMAD2 SMAD family member 2 XM_024451173.1 100% 100%
7 human 4087 SMAD2 SMAD family member 2 NM_001135937.2 93.5% 93.5% 234_235ins90
8 human 4087 SMAD2 SMAD family member 2 XM_017025746.2 93.5% 93.5% 234_235ins90
9 human 4087 SMAD2 SMAD family member 2 XM_011525984.2 84.8% 82.1% (many diffs)
10 human 4087 SMAD2 SMAD family member 2 XM_011525985.3 83.9% 81.3% (many diffs)
11 human 4087 SMAD2 SMAD family member 2 XM_017025748.2 83.9% 81.3% (many diffs)
12 human 4087 SMAD2 SMAD family member 2 XM_017025747.2 80.3% 80.6% 0_1ins218;19_89del
13 human 4088 SMAD3 SMAD family member 3 NM_005902.4 69.3% 83.9% (many diffs)
14 human 4088 SMAD3 SMAD family member 3 XM_011521559.3 62% 75.3% (many diffs)
15 human 4087 SMAD2 SMAD family member 2 XM_017025749.1 54.3% 52.2% (many diffs)
16 human 4087 SMAD2 SMAD family member 2 XM_017025750.2 48.6% 48.6% 0_1ins720
17 mouse 17126 Smad2 SMAD family member 2 NM_001252481.1 92.2% 99.5% (many diffs)
18 mouse 17126 Smad2 SMAD family member 2 NM_010754.5 92.2% 99.5% (many diffs)
19 mouse 17126 Smad2 SMAD family member 2 XM_006525699.2 92.2% 99.5% (many diffs)
20 mouse 17126 Smad2 SMAD family member 2 NM_001311070.1 86% 93.5% (many diffs)
21 mouse 17126 Smad2 SMAD family member 2 XM_017317842.1 86% 93.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1467
ORF length:
1401
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc gtccatcttg ccattcacgc cgccagttgt gaagagactg ctgggatgga
121agaagtcagc tggtgggtct ggaggagcag gcggaggaga gcagaatggg caggaagaaa
181agtggtgtga gaaagcagtg aaaagtctgg tgaagaagct aaagaaaaca ggacgattag
241atgagcttga gaaagccatc accactcaaa actgtaatac taaatgtgtt accataccaa
301gcacttgctc tgaaatttgg ggactgagta caccaaatac gatagatcag tgggatacaa
361caggccttta cagcttctct gaacaaacca ggtctcttga tggtcgtctc caggtatccc
421atcgaaaagg attgccacat gttatatatt gccgattatg gcgctggcct gatcttcaca
481gtcatcatga actcaaggca attgaaaact gcgaatatgc ttttaatctt aaaaaggatg
541aagtatgtgt aaacccttac cactatcaga gagttgagac accagttttg cctccagtat
601tagtgccccg acacaccgag atcctaacag aacttccgcc tctggatgac tatactcact
661ccattccaga aaacactaac ttcccagcag gaattgagcc acagagtaat tatattccag
721aaacgccacc tcctggatat atcagtgaag atggagaaac aagtgaccaa cagttgaatc
781aaagtatgga cacaggctct ccagcagaac tatctcctac tactctttcc cctgttaatc
841atagcttgga tttacagcca gttacttact cagaacctgc attttggtgt tcgatagcat
901attatgaatt aaatcagagg gttggagaaa ccttccatgc atcacagccc tcactcactg
961tagatggctt tacagaccca tcaaattcag agaggttctg cttaggttta ctctccaatg
1021ttaaccgaaa tgccacggta gaaatgacaa gaaggcatat aggaagagga gtgcgcttat
1081actacatagg tggggaagtt tttgctgagt gcctaagtga tagtgcaatc tttgtgcaga
1141gccccaattg taatcagaga tatggctggc accctgcaac agtgtgtaaa attccaccag
1201gctgtaatct gaagatcttc aacaaccagg aatttgctgc tcttctggct cagtctgtta
1261atcagggttt tgaagccgtc tatcagctaa ctagaatgtg caccataaga atgagttttg
1321tgaaagggtg gggagcagaa taccgaaggc agacggtaac aagtactcct tgctggattg
1381aacttcatct gaatggacct ctacagtggt tggacaaagt attaactcag atgggatccc
1441cttcagtgcg ttgctcaagc atgtcatacc caactttctt gtacaaagtg gttggtaagc
1501ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
1561ggcgcgttaa gtcgacaatc aacctctgga tta

Download FASTA (ORF) (Full)