Construct: ORF ccsbBroad304_00972

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013842.1_s304c1, BRDN0000404836
Derived from:
ccsbBroadEn_00972
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAN2C1 (4123)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00972
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4123 MAN2C1 mannosidase alpha class 2C ... NM_006715.4 100% 100%
2 human 4123 MAN2C1 mannosidase alpha class 2C ... NM_001256494.1 98.3% 94.6% 1947_1959del;2058_2095del
3 human 4123 MAN2C1 mannosidase alpha class 2C ... NM_001256495.1 97.7% 97.7% 2736_2737ins69
4 human 4123 MAN2C1 mannosidase alpha class 2C ... NM_001256496.1 90.4% 90.4% 598_599ins297
5 mouse 73744 Man2c1 mannosidase, alpha, class 2... NM_028636.2 87% 89.9% (many diffs)
6 mouse 73744 Man2c1 mannosidase, alpha, class 2... XM_006511499.3 84% 84.3% (many diffs)
7 mouse 73744 Man2c1 mannosidase, alpha, class 2... XM_006511495.3 82.6% 85.2% (many diffs)
8 mouse 73744 Man2c1 mannosidase, alpha, class 2... XM_006511494.3 81.8% 84.4% (many diffs)
9 mouse 73744 Man2c1 mannosidase, alpha, class 2... XM_006511493.3 79.6% 82.1% (many diffs)
10 mouse 73744 Man2c1 mannosidase, alpha, class 2... XM_017313619.1 78.9% 81.3% (many diffs)
11 mouse 73744 Man2c1 mannosidase, alpha, class 2... XM_017313622.1 77% 78.5% (many diffs)
12 mouse 73744 Man2c1 mannosidase, alpha, class 2... XM_011242824.2 76.9% 79.1% (many diffs)
13 mouse 73744 Man2c1 mannosidase, alpha, class 2... XM_017313621.1 74.9% 76.2% (many diffs)
14 mouse 73744 Man2c1 mannosidase, alpha, class 2... XM_017313624.1 74% 75.2% (many diffs)
15 mouse 73744 Man2c1 mannosidase, alpha, class 2... XM_017313620.1 72.1% 73.4% (many diffs)
16 mouse 73744 Man2c1 mannosidase, alpha, class 2... XM_017313623.1 72% 73% (many diffs)
17 mouse 73744 Man2c1 mannosidase, alpha, class 2... XM_006511496.2 69% 71.4% (many diffs)
18 mouse 73744 Man2c1 mannosidase, alpha, class 2... XR_001779030.1 68.6% (many diffs)
19 mouse 73744 Man2c1 mannosidase, alpha, class 2... XR_001779025.1 66.3% (many diffs)
20 mouse 73744 Man2c1 mannosidase, alpha, class 2... XM_017313626.1 66.1% 68% (many diffs)
21 mouse 73744 Man2c1 mannosidase, alpha, class 2... XR_001779027.1 64.6% (many diffs)
22 mouse 73744 Man2c1 mannosidase, alpha, class 2... XR_001779028.1 64.6% (many diffs)
23 mouse 73744 Man2c1 mannosidase, alpha, class 2... XM_017313625.1 64.3% 66.1% (many diffs)
24 mouse 73744 Man2c1 mannosidase, alpha, class 2... XR_001779026.1 64.3% (many diffs)
25 mouse 73744 Man2c1 mannosidase, alpha, class 2... XR_001779038.1 64% (many diffs)
26 mouse 73744 Man2c1 mannosidase, alpha, class 2... XR_001779029.1 62.2% (many diffs)
27 mouse 73744 Man2c1 mannosidase, alpha, class 2... XR_001779033.1 61.8% (many diffs)
28 mouse 73744 Man2c1 mannosidase, alpha, class 2... XR_001779034.1 61.6% (many diffs)
29 mouse 73744 Man2c1 mannosidase, alpha, class 2... XR_001779032.1 60.7% (many diffs)
30 mouse 73744 Man2c1 mannosidase, alpha, class 2... XR_001779031.1 58.9% (many diffs)
31 mouse 73744 Man2c1 mannosidase, alpha, class 2... XR_001779040.1 58.9% (many diffs)
32 mouse 73744 Man2c1 mannosidase, alpha, class 2... XR_001779039.1 58.8% (many diffs)
33 mouse 73744 Man2c1 mannosidase, alpha, class 2... XR_379466.3 57.3% (many diffs)
34 mouse 73744 Man2c1 mannosidase, alpha, class 2... XR_001779036.1 57.1% (many diffs)
35 mouse 73744 Man2c1 mannosidase, alpha, class 2... XR_001779037.1 57.1% (many diffs)
36 mouse 73744 Man2c1 mannosidase, alpha, class 2... XR_001779035.1 54.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3186
ORF length:
3120
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggctgcgccg gccttgaagc actggcgcac cacgctggag cgggtggaga
121agttcgtgtc gccgctctac tttaccgact gtaacctccg cggcaggctt tttggggcca
181gctgccctgt ggctgtgctc tccagcttcc tgacgccgga gagacttccc taccaggagg
241cagtccagcg ggacttccgc cccgcgcagg tcggcgacag cttcggaccc acatggtgga
301cctgctggtt ccgggtggag ctgaccatcc cagaggcatg ggtgggccag gaagttcacc
361tttgctggga aagtgatgga gaaggtctgg tgtggcgtga tggagaacct gtccagggtt
421taaccaaaga gggtgagaag accagctatg tcctgactga caggctgggg gaaagagacc
481cccgaagcct cactctctat gtggaagtag cctgcaatgg gctcctgggg gccgggaagg
541gaagcatgat tgcagcccct gaccctgaga agatgttcca gctgagccgg gctgagctag
601ctgtgttcca ccgggatgtc cacatgctcc tggtggatct ggagctgctg ctgggcatag
661ccaagggcct cgggaaggac aaccagcgca gcttccaggc cctgtacaca gccaatcaga
721tggtgaacgt gtgtgaccct gcccagcccg agaccttccc agtggcccag gccctggcct
781ccaggttctt tggccaacat gggggtgaaa gccaacacac cattcatgcc acagggcact
841gccacattga tacagcctgg ctttggccct tcaaagagac tgtgaggaaa tgtgcccgga
901gctgggtgac cgccctgcag ctcatggagc ggaaccctga gttcatcttt gcctgctccc
961aggcgcagca gctggaatgg gtgaagagcc gctaccctgg cctgtactcc cgcatccagg
1021agtttgcgtg ccgtgggcag tttgtgcctg tggggggcac ctgggtggag atggatggga
1081acctgcccag tggagaggcc atggtgaggc agtttttgca gggccagaac ttctttctgc
1141aggagtttgg gaagatgtgc tctgagttct ggctgccgga cacctttggc tactcagcac
1201agctccccca gatcatgcac ggctgtggca tcaggcgctt tctcacccag aaattgagct
1261ggaatttggt gaactccttc ccacaccata catttttctg ggagggcctg gatggctccc
1321gtgtactggt ccacttccca cctggcgact cctatgggat gcagggcagc gtggaggagg
1381tgctgaagac cgtggccaac aaccgggaca aggggcgggc caaccacagt gccttcctct
1441ttggctttgg ggatgggggt ggtggcccca cccagaccat gctggaccgc ctgaagcgcc
1501tgagcaatac ggatgggctg cccagggtgc agctatcttc tccaagacag ctcttctcag
1561cactggagag tgactcagag cagctgtgca cgtgggttgg ggagctcttc ttggagctgc
1621acaatggcac atacaccacc catgcccaga tcaagaaggg gaaccgggaa tgtgagcgga
1681tcctgcacga cgtggagctg ctcagtagcc tggccctggc ccgcagtgcc cagttcctat
1741acccagcagc ccagctgcag cacctctgga ggctccttct tctgaaccag ttccatgatg
1801tggtgactgg aagctgcatc cagatggtgg cagaggaagc catgtgccat tatgaagaca
1861tccgttccca tggcaataca ctgctcagcg ctgcagccgc agccctgtgt gctggggagc
1921caggtcctga gggcctcctc atcgtcaaca cactgccctg gaagcggatc gaagtgatgg
1981ccctgcccaa accgggcggg gcccacagcc tagccctggt gacagtgccc agcatgggct
2041atgctcctgt tcctcccccc acctcactgc agcccctgct gccccagcag cctgtgttcg
2101tagtgcaaga gactgatggc tccgtgactc tggacaatgg catcatccga gtgaagctgg
2161acccaactgg tcgcctgacg tccttggtcc tggtggcctc tggcagggag gccattgctg
2221agggcgccgt ggggaaccag tttgtgctat ttgatgatgt ccccttgtac tgggatgcat
2281gggacgtcat ggactaccac ctggagacac ggaagcctgt gctgggccag gcagggaccc
2341tggcagtggg caccgagggc ggcctgcggg gcagcgcctg gttcttgcta cagatcagcc
2401ccaacagtcg gcttagccag gaggttgtgc tggacgttgg ctgcccctat gtccgcttcc
2461acaccgaggt acactggcat gaggcccaca agttcctgaa ggtggagttc cctgctcgcg
2521tgcggagttc ccaggccacc tatgagatcc agtttgggca cctgcagcga cctacccact
2581acaatacctc ttgggactgg gctcgatttg aggtgtgggc ccatcgctgg atggatctgt
2641cagaacacgg ctttgggctg gccctgctca acgactgcaa gtatggcgcg tcagtgcgag
2701gcagcatcct cagcctctcg ctcttgcggg cgcctaaagc cccggacgct actgctgaca
2761cggggcgcca cgagttcacc tatgcactga tgccgcacaa gggctctttc caggatgctg
2821gcgttatcca agctgcctac agcctaaact tccccctgtt ggctctgcca gcccccagcc
2881cagcgcccgc cacctcctgg agtgcgtttt ccgtgtcttc acccgcggtc gtattggaga
2941ccgtcaagca ggcggagagc agcccccagc gccgctcgct ggtcctgagg ctgtatgagg
3001cccacggcag ccacgtggac tgctggctgc acttgtcgct gccggttcag gaggccatcc
3061tctgcgatct cttggagcga ccagaccctg ctggccactt gacccttcgg gacaaccgcc
3121tgaagctcac cttttctccc ttccaagtgc tgtccctgtt gctcgtgctt cagcctccgc
3181cacactgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
3241gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
3301acctctggat ta

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