Transcript: Mouse XR_001779035.1

PREDICTED: Mus musculus mannosidase, alpha, class 2C, member 1 (Man2c1), transcript variant X25, misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Man2c1 (73744)
Length:
3152
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001779035.1
NBCI Gene record:
Man2c1 (73744)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_001779035.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000077091 GTTTGACCAAAGAAGGCGAAA pLKO.1 990 3UTR 100% 4.050 3.240 N Man2c1 n/a
2 TRCN0000334212 GTTTGACCAAAGAAGGCGAAA pLKO_005 990 3UTR 100% 4.050 3.240 N Man2c1 n/a
3 TRCN0000348295 TGGTGACAGTGCCAAGTATTG pLKO_005 2590 3UTR 100% 10.800 7.560 N Man2c1 n/a
4 TRCN0000049612 CGATCTACTTCACCGACTGTA pLKO.1 708 3UTR 100% 4.950 3.465 N MAN2C1 n/a
5 TRCN0000077092 GCTACCTATGAGATCCAGTTT pLKO.1 3013 3UTR 100% 4.950 3.465 N Man2c1 n/a
6 TRCN0000334214 GCTACCTATGAGATCCAGTTT pLKO_005 3013 3UTR 100% 4.950 3.465 N Man2c1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001779035.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00972 pDONR223 100% 54.3% None (many diffs) n/a
2 ccsbBroad304_00972 pLX_304 0% 54.3% V5 (many diffs) n/a
3 TRCN0000468851 CATTCGAGTAAATACTCTAGCGTC pLX_317 13.2% 54.3% V5 (many diffs) n/a
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