Construct: ORF ccsbBroad304_01027
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013729.1_s304c1, BRDN0000403322
- Derived from:
- ccsbBroadEn_01027
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MOBP (4336)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01027
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4336 | MOBP | myelin associated oligodend... | NM_182935.4 | 100% | 100% | |
| 2 | human | 4336 | MOBP | myelin associated oligodend... | NM_001278323.1 | 40.4% | 38.7% | (many diffs) |
| 3 | human | 4336 | MOBP | myelin associated oligodend... | NM_001278322.2 | 37.9% | 34.9% | (many diffs) |
| 4 | human | 4336 | MOBP | myelin associated oligodend... | NR_003090.3 | 4.2% | 1_149del;393_5717del | |
| 5 | human | 4336 | MOBP | myelin associated oligodend... | NR_103504.2 | 3.7% | (many diffs) | |
| 6 | human | 4336 | MOBP | myelin associated oligodend... | NR_103505.2 | 3.7% | (many diffs) | |
| 7 | mouse | 17433 | Mobp | myelin-associated oligodend... | NM_001039364.2 | 87.6% | 90.1% | (many diffs) |
| 8 | mouse | 17433 | Mobp | myelin-associated oligodend... | NM_008614.2 | 87.6% | 90.1% | (many diffs) |
| 9 | mouse | 17433 | Mobp | myelin-associated oligodend... | NM_001039365.2 | 40.8% | 39.4% | (many diffs) |
| 10 | mouse | 17433 | Mobp | myelin-associated oligodend... | XR_379851.1 | 8.4% | (many diffs) | |
| 11 | mouse | 17433 | Mobp | myelin-associated oligodend... | XR_871174.1 | 7.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 309
- ORF length:
- 243
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag tcagaaaccg gccaaggagg gtcccagact ctccaaaaac cagaagtact 121 ccgaacactt cagcatacac tgctgcccgc cgttcacctt cctcaattcc aagaaggaga 181 tagtggatcg gaaatacagc atctgtaaga gcggctgctt ctaccagaag aaagaggagg 241 actggatctg ctgcgcctgc cagaagacca gattgaaaag gaagatcagg ccaaccccaa 301 agaagaagtg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 361 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 421 tcaacctctg gatta