Construct: ORF ccsbBroad304_01052
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012147.1_s304c1, BRDN0000461712
- Derived from:
- ccsbBroadEn_01052
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYB (4602)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01052
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4602 | MYB | MYB proto-oncogene, transcr... | NM_005375.4 | 100% | 100% | |
| 2 | human | 4602 | MYB | MYB proto-oncogene, transcr... | NM_001130172.2 | 99.5% | 99.5% | 939_940insGTGCTACCA |
| 3 | human | 4602 | MYB | MYB proto-oncogene, transcr... | NM_001161660.2 | 94.5% | 94.5% | 843_844ins105 |
| 4 | human | 4602 | MYB | MYB proto-oncogene, transcr... | NM_001161659.2 | 94.2% | 94.2% | 1586_1587ins111 |
| 5 | human | 4602 | MYB | MYB proto-oncogene, transcr... | NM_001161657.2 | 86.7% | 86.7% | 948_949ins255 |
| 6 | human | 4602 | MYB | MYB proto-oncogene, transcr... | NM_001161658.2 | 85.7% | 84.4% | (many diffs) |
| 7 | human | 4602 | MYB | MYB proto-oncogene, transcr... | NM_001130173.2 | 84% | 84% | 1204_1566del |
| 8 | human | 4602 | MYB | MYB proto-oncogene, transcr... | NM_001161656.2 | 83.7% | 83.7% | 939_940insGTGCTACCA;1195_1557del |
| 9 | human | 4602 | MYB | MYB proto-oncogene, transcr... | XR_942444.2 | 57.5% | (many diffs) | |
| 10 | human | 4602 | MYB | MYB proto-oncogene, transcr... | NR_134960.2 | 56.8% | 1_206del;1553_1608del;2183_3377del | |
| 11 | human | 4602 | MYB | MYB proto-oncogene, transcr... | NR_134964.2 | 56.5% | (many diffs) | |
| 12 | human | 4602 | MYB | MYB proto-oncogene, transcr... | NR_134959.2 | 55.8% | (many diffs) | |
| 13 | human | 4602 | MYB | MYB proto-oncogene, transcr... | NR_134958.2 | 55.7% | 1_206del;1152_1273del;2249_3443del | |
| 14 | human | 4602 | MYB | MYB proto-oncogene, transcr... | NR_134963.2 | 55.4% | 1_206del;1792_1933del;2269_3463del | |
| 15 | human | 4602 | MYB | MYB proto-oncogene, transcr... | NR_134965.2 | 54.8% | (many diffs) | |
| 16 | human | 4602 | MYB | MYB proto-oncogene, transcr... | NR_134961.2 | 54.7% | 1_206del;1410_1592del;2310_3504del | |
| 17 | human | 4602 | MYB | MYB proto-oncogene, transcr... | NR_134962.2 | 49.3% | (many diffs) | |
| 18 | mouse | 17863 | Myb | myeloblastosis oncogene | NM_010848.3 | 87.2% | 90.4% | (many diffs) |
| 19 | mouse | 17863 | Myb | myeloblastosis oncogene | NM_001198914.1 | 73.5% | 76.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1986
- ORF length:
- 1920
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ccgaagaccc cggcacagca tatatagcag tgacgaggat gatgaggact 121 ttgagatgtg tgaccatgac tatgatgggc tgcttcccaa gtctggaaag cgtcacttgg 181 ggaaaacaag gtggacccgg gaagaggatg aaaaactgaa gaagctggtg gaacagaatg 241 gaacagatga ctggaaagtt attgccaatt atctcccgaa tcgaacagat gtgcagtgcc 301 agcaccgatg gcagaaagta ctaaaccctg agctcatcaa gggtccttgg accaaagaag 361 aagatcagag agtgatagag cttgtacaga aatacggtcc gaaacgttgg tctgttattg 421 ccaagcactt aaaggggaga attggaaaac aatgtaggga gaggtggcat aaccacttga 481 atccagaagt taagaaaacc tcctggacag aagaggaaga cagaattatt taccaggcac 541 acaagagact ggggaacaga tgggcagaaa tcgcaaagct actgcctgga cgaactgata 601 atgctatcaa gaaccactgg aattctacaa tgcgtcggaa ggtcgaacag gaaggttatc 661 tgcaggagtc ttcaaaagcc agccagccag cagtggccac aagcttccag aagaacagtc 721 atttgatggg ttttgctcag gctccgccta cagctcaact ccctgccact ggccagccca 781 ctgttaacaa cgactattcc tattaccaca tttctgaagc acaaaatgtc tccagtcatg 841 ttccataccc tgtagcgtta catgtaaata tagtcaatgt ccctcagcca gctgccgcag 901 ccattcagag acactataat gatgaagacc ctgagaagga aaagcgaata aaggaattag 961 aattgctcct aatgtcaacc gagaatgagc taaaaggaca gcaggtgcta ccaacacaga 1021 accacacatg cagctacccc gggtggcaca gcaccaccat tgccgaccac accagacctc 1081 atggagacag tgcacctgtt tcctgtttgg gagaacacca ctccactcca tctctgccag 1141 cggatcctgg ctccctacct gaagaaagcg cctcgccagc aaggtgcatg atcgtccacc 1201 agggcaccat tctggataat gttaagaacc tcttagaatt tgcagaaaca ctccaattta 1261 tagattcttt cttaaacact tccagtaacc atgaaaactc agacttggaa atgccttctt 1321 taacttccac ccccctcatt ggtcacaaat tgactgttac aacaccattt catagagacc 1381 agactgtgaa aactcaaaag gaaaatactg tttttagaac cccagctatc aaaaggtcaa 1441 tcttagaaag ctctccaaga actcctacac cattcaaaca tgcacttgca gctcaagaaa 1501 ttaaatacgg tcccctgaag atgctacctc agacaccctc tcatctagta gaagatctgc 1561 aggatgtgat caaacaggaa tctgatgaat ctggaattgt tgctgagttt caagaaaatg 1621 gaccaccctt actgaagaaa atcaaacaag aggtggaatc tccaactgat aaatcaggaa 1681 acttcttctg ctcacaccac tgggaagggg acagtctgaa tacccaactg ttcacgcaga 1741 cctcgcctgt ggcagatgca ccgaatattc ttacaagctc cgttttaatg gcaccagcat 1801 cagaagatga agacaatgtt ctcaaagcat ttacagtacc taaaaacagg tccctggcga 1861 gccccttgca gccttgtagc agtacctggg aacctgcatc ctgtggaaag atggaggagc 1921 agatgacatc ttccagtcaa gctcgtaaat acgtgaatgc attctcagcc cggacgctgg 1981 tcatgtgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 2041 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 2101 acctctggat ta