Construct: ORF ccsbBroad304_01255

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006753.1_s304c1, BRDN0000399327
Derived from:
ccsbBroadEn_01255
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PPM1B (5495)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01255
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5495 PPM1B protein phosphatase, Mg2+/M... NM_177969.3 100% 100%
2 human 5495 PPM1B protein phosphatase, Mg2+/M... NM_002706.6 40% 40% 1_861del
3 human 5495 PPM1B protein phosphatase, Mg2+/M... XM_017004395.2 39.4% 39.4% 1_861del;963_986del
4 human 5495 PPM1B protein phosphatase, Mg2+/M... XM_011532936.3 26.8% 21.2% (many diffs)
5 human 5495 PPM1B protein phosphatase, Mg2+/M... XM_017004396.2 26.4% 20.9% (many diffs)
6 human 5495 PPM1B protein phosphatase, Mg2+/M... NM_177968.4 20% 19.2% (many diffs)
7 human 5495 PPM1B protein phosphatase, Mg2+/M... XM_017004397.2 19.7% 18.8% (many diffs)
8 human 5495 PPM1B protein phosphatase, Mg2+/M... NM_001033557.3 19.4% 18.9% (many diffs)
9 mouse 19043 Ppm1b protein phosphatase 1B, mag... NM_001159496.1 35.9% 34.6% (many diffs)
10 mouse 19043 Ppm1b protein phosphatase 1B, mag... XM_006523860.3 35.9% 34.6% (many diffs)
11 mouse 19043 Ppm1b protein phosphatase 1B, mag... XM_011246321.2 35.9% 34.6% (many diffs)
12 mouse 19043 Ppm1b protein phosphatase 1B, mag... XM_011246322.2 35.9% 34.6% (many diffs)
13 mouse 19043 Ppm1b protein phosphatase 1B, mag... NM_001159498.1 19.1% 17.5% (many diffs)
14 mouse 19043 Ppm1b protein phosphatase 1B, mag... XM_006523861.3 19.1% 17.5% (many diffs)
15 mouse 19043 Ppm1b protein phosphatase 1B, mag... XM_011246323.1 19.1% 17.5% (many diffs)
16 mouse 19043 Ppm1b protein phosphatase 1B, mag... NM_011151.2 19% 18.1% (many diffs)
17 mouse 19043 Ppm1b protein phosphatase 1B, mag... NM_001159497.1 18.9% 17.5% (many diffs)
18 mouse 19043 Ppm1b protein phosphatase 1B, mag... XM_006523862.3 18.9% 17.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
642
ORF length:
576
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag tattgtacta gtttgctttt caaatgctcc caaggtctca gatgaagcgg
121tgaaaaaaga ttcagagttg gataagcact tggaatcacg ggttgaagag attatggaga
181agtctggcga ggaaggaatg cctgatcttg cccatgtcat gcgcatcttg tctgcagaaa
241atatcccaaa tttgcctcct gggggaggtc ttgctggcaa gcgtaatgtt attgaagctg
301tttatagtag actgaatcca catagagaaa gtgatggggc ctccgatgaa gcagaggaaa
361gtggatcaca gggaaaattg gtggaagctc tcaggcaaat gagaattaat cataggggaa
421actaccgaca acttctggag gagatgctga ctagttacag gctagctaaa gtagagggag
481aagaaagccc tgctgaacca gctgccacag ctacttcttc gaacagtgat gctggaaacc
541cagtgacaat gcaggaaagc catactgaat cagaaagtgg tcttgctgaa ttagacagct
601ctaatgaaga tgcagggaca aagatgagtg gtgaaaaaat atgcccaact ttcttgtaca
661aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
721ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)