Construct: ORF ccsbBroad304_01281

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000904.1_s304c1, BRDN0000411671
Derived from:
ccsbBroadEn_01281
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PRKCA (5578)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01281
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5578 PRKCA protein kinase C alpha NM_002737.3 99.9% 67.4% 1357delT;1702G>A
2 human 5578 PRKCA protein kinase C alpha XM_017024836.2 93.2% 70.2% (many diffs)
3 human 5578 PRKCA protein kinase C alpha XM_017024837.1 91.2% 57.4% (many diffs)
4 human 5578 PRKCA protein kinase C alpha XM_024450829.1 86.3% 53.5% (many diffs)
5 human 5578 PRKCA protein kinase C alpha XM_024450830.1 86.3% 53.5% (many diffs)
6 human 5578 PRKCA protein kinase C alpha XR_001752558.1 55.7% (many diffs)
7 human 5578 PRKCA protein kinase C alpha XM_017024841.1 47.2% 66.2% (many diffs)
8 mouse 18750 Prkca protein kinase C, alpha NM_011101.3 91% 67% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1458
ORF length:
1389
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggctgacgtt ttcccgggca acgactccac ggcgtctcag gacgtggcca
121accgcttcgc ccgcaaaggg gcgctgaggc agaagaacgt gcacgaggtg aaggaccaca
181aattcatcgc gcgcttcttc aagcagccca ccttctgcag ccactgcacc gacttcatct
241gggggtttgg gaaacaaggc ttccagtgcc aagtttgctg ttttgtggtc cacaagaggt
301gccatgaatt tgttactttt tcttgtccgg gtgcggataa gggacccgac actgatgacc
361ccaggagcaa gcacaagttc aaaatccaca cttacggaag ccccaccttc tgcgatcact
421gtgggtcact gctctatgga cttatccatc aagggatgaa atgtgacacc tgcgatatga
481acgttcacaa gcaatgcgtc atcaatgtcc ccagcctctg cggaatggat cacactgaga
541agagggggcg gatttaccta aaggctgagg ttgctgatga aaagctccat gtcacagtac
601gagatgcaaa aaatctaatc cctatggatc caaacgggct ttcagatcct tatgtgaagc
661tgaaacttat tcctgatccc aagaatgaaa gcaagcaaaa aaccaaaacc atccgctcca
721cactaaatcc gcagtggaat gagtccttta cattcaaatt gaaaccttca gacaaagacc
781gacgactgtc tgtagaaatc tgggactggg atcgaacaac aaggaatgac ttcatgggat
841ccctttcctt tggagtttcg gagctgatga agatgccggc cagtggatgg tacaagttgc
901ttaaccaaga agaaggtgag tactacaacg tacccattcc ggaaggggac gaggaaggaa
961acatggaact caggcagaaa ttcgagaaag ccaaacttgg ccctgctggc aacaaagtca
1021tcagtccctc tgaagacagg aaacaacctt ccaacaacct tgaccgagtg aaactcacgg
1081acttcaattt cctcatggtg ttgggaaagg ggagttttgg aaaggtgatg cttgccgaca
1141ggaagggcac agaagaactg tatgcaatca aaatcctgaa gaaggatgtg gtgattcagg
1201atgatgacgt ggagtgcacc atggtagaaa agcgagtctt ggccctgctt gacaaacccc
1261cgttcttgac gcagctgcac tcctgcttcc agacagtgga tcggctgtac ttcgtcatgg
1321aatatgtcaa cggtggggac ctcatgtacc acattcagca agtaggaaaa tttaaggaac
1381cacaagcagt attctatgcg gcagagatTT CCATCGGATT GTTCTTCTTC ATAAAAGAGG
1441AATCATTTAT AGGGATCTGA AGTTAGATAA CGTCATGTTG GATTCAGAAG GACATATCAA
1501AATTGCTGAC TTTGGGATGT GCAAGGAACA CATGATGGAT GGAGTCACGA CCAGGACCTT
1561CTGTGGGACT CCAGATTATA TCGCCCCAGA GATAATCGCT TATCAGCCGT ATGGAAAATC
1621TGTGGACTGG TGGGCCTATG GCGTCCTGTT GTATGAAATG CTTGCCGGGC AGCCTCCATT
1681TGATGGTGAA GATGAAGACG AGCTATTTCA GTCTATCATG GAGCACAACG TTTCCTATCC
1741AAAATCCTTG TCCAAGGAGG CTGTTTCTAT CTGCAAAGGA CTGATGACCA AACACCCAGC
1801CAAGCGGCTG GGCTGTGGGC CTGAGGGGGA GAGGGACGTG AGAGAGCATG CCTTCTTCCG
1861GAGGATCGAC TGGGAAAAAC TGGAGAACAG GGAGATCCAG CCACCATTCA AGCCCAAAGT
1921GTGTGGCAAA GGAGCAGAGA ACTTTGACAA GTTCTTCACA CGAGGACAGC CCGTCTTAAC
1981ACCACCTGAT CAGCTGGTTA TTGCTAACAT AGACCAGTCT GATTTTGAAG GGTTCTCGTA
2041TGTCAACCCC CAGTTTGTGC ACCCCATCTT ACAGAGTGCA GTATTGCCAA CTTTCTTGTA
2101Caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg
2161agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)