Construct: ORF ccsbBroad304_01398

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011404.1_s304c1, BRDN0000407092
Derived from:
ccsbBroadEn_01398
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RHEB (6009)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01398
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6009 RHEB Ras homolog, mTORC1 binding NM_005614.4 100% 100%
2 human 6009 RHEB Ras homolog, mTORC1 binding XM_011516457.2 91.5% 91.3% (many diffs)
3 human 6009 RHEB Ras homolog, mTORC1 binding XM_024446854.1 91.5% 91.3% (many diffs)
4 human 439965 SHLD2P3 shieldin complex subunit 2 ... NR_160660.1 23.1% (many diffs)
5 human 439965 SHLD2P3 shieldin complex subunit 2 ... NR_160661.1 21.3% (many diffs)
6 mouse 19744 Rheb Ras homolog enriched in brain NM_053075.3 92.5% 98.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
618
ORF length:
552
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcATGCC GCAGTCCAAG TCCCGGAAGA TCGCGATCCT GGGCTACCGG TCTGTGGGGA
121AATCCTCATT GACGATTCAA TTTGTTGAAG GCCAATTTGT GGACTCCTAC GATCCAACCA
181TAGAAAACAC TTTTACAAAG TTGATCACAG TAAATGGACA AGAATATCAT CTTCAACTTG
241TAGACACAGC CGGGCAAGAT GAATATTCTA TCTTTCCTCA GACATACTCC ATAGATATTA
301ATGGCTATAT TCTTGTGTAT TCTGTTACAT CAATCAAAAG TTTTGAAGTG ATTAAAGTTA
361TCCATGGCAA ATTGTTGGAT ATGGTGGGGA AAGTACAAAT ACCTATTATG TTGGTTGGGA
421ATAAGAAAGA CCTGCATATG GAAAGGGTGA TCAGTTATGA AGAAGGGAAA GCTTTGGCAG
481AATCTTGGAA TGCAGCTTTT TTGGAATCTT CTGCTAAAGA AAATCAGACT GCTGTGGATG
541TTTTTCGAAG GATAATTTTG GAGGCAGAAA AAATGGACGG GGCAGCTTCA CAAGGCAAGT
601CTTCATGCTC GGTGATGTGC CCAACTTTCT TGTACaaagt ggttggtaag cctatcccta
661accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
721agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)