Construct: ORF ccsbBroad304_01735

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003146.1_s304c1, BRDN0000409747
Derived from:
ccsbBroadEn_01735
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
UBE2D1 (7321)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01735
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7321 UBE2D1 ubiquitin conjugating enzym... NM_003338.5 100% 100%
2 human 51619 UBE2D4 ubiquitin conjugating enzym... NM_015983.4 77.5% 91.8% (many diffs)
3 human 7321 UBE2D1 ubiquitin conjugating enzym... NM_001204880.1 73.9% 72.7% 0_1ins111;2_3insGGG;4A>C
4 human 7323 UBE2D3 ubiquitin conjugating enzym... NM_001300795.1 64.7% 70.7% (many diffs)
5 human 7323 UBE2D3 ubiquitin conjugating enzym... XM_017008584.2 64.7% 70.7% (many diffs)
6 human 51619 UBE2D4 ubiquitin conjugating enzym... XM_024446796.1 57.3% 68% (many diffs)
7 mouse 216080 Ube2d1 ubiquitin-conjugating enzym... NM_145420.2 89.3% 100% (many diffs)
8 mouse 216080 Ube2d1 ubiquitin-conjugating enzym... XM_006513478.2 81.4% 89.3% (many diffs)
9 mouse 216080 Ube2d1 ubiquitin-conjugating enzym... XM_006513479.2 66.6% 73.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
507
ORF length:
441
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc gctgaagagg attcagaaag aattgagtga tctacagcgc gatccacctg
121ctcactgttc agctggacct gtgggagatg acttgttcca ctggcaagcc actattatgg
181ggcctcctga tagcgcatat caaggtggag tcttctttct cactgtacat tttccgacag
241attatccttt taaaccacca aagattgctt tcacaacaaa aatttaccat ccaaacataa
301acagtaatgg aagtatttgt ctcgatattc tgaggtcaca atggtcacca gctctgactg
361tatcaaaagt tttattgtcc atatgttctc tactttgtga tcctaatcca gatgacccct
421tagtaccaga tattgcacaa atctataaat cagacaaaga aaaatacaac agacatgcaa
481gagaatggac tcagaaatat gcaatgtacc caactttctt gtacaaagtg gttggtaagc
541ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
601ggcgcgttaa gtcgacaatc aacctctgga tta

Download FASTA (ORF) (Full)