Construct: ORF ccsbBroad304_01778
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000401.1_s304c1, BRDN0000410098
- Derived from:
- ccsbBroadEn_01778
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WNT2 (7472)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01778
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7472 | WNT2 | Wnt family member 2 | NM_003391.3 | 100% | 100% | |
| 2 | human | 7472 | WNT2 | Wnt family member 2 | NR_024047.2 | 26.3% | 1_69del;379_380ins64;1086_3792del | |
| 3 | mouse | 22413 | Wnt2 | wingless-type MMTV integrat... | NM_023653.5 | 91.7% | 96.3% | (many diffs) |
| 4 | mouse | 22413 | Wnt2 | wingless-type MMTV integrat... | XM_006505045.2 | 82.4% | 85.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1146
- ORF length:
- 1080
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa cgcccctctc ggtggaatct ggctctggct ccctctgctc ttgacctggc 121 tcacccccga ggtcaactct tcatggtggt acatgagagc tacaggtggc tcctccaggg 181 tgatgtgcga taatgtgcca ggcctggtga gcagccagcg gcagctgtgt caccgacatc 241 cagatgtgat gcgtgccatt agccagggcg tggccgagtg gacagcagaa tgccagcacc 301 agttccgcca gcaccgctgg aattgcaaca ccctggacag ggatcacagc ctttttggca 361 gggtcctact ccgaagtagt cgggaatctg cctttgttta tgccatctcc tcagctggag 421 ttgtatttgc catcaccagg gcctgtagcc aaggagaagt aaaatcctgt tcctgtgatc 481 caaagaagat gggaagcgcc aaggacagca aaggcatttt tgattggggt ggctgcagtg 541 ataacattga ctatgggatc aaatttgccc gcgcatttgt ggatgcaaag gaaaggaaag 601 gaaaggatgc cagagccctg atgaatcttc acaacaacag agctggcagg aaggctgtaa 661 agcggttctt gaaacaagag tgcaagtgcc acggggtgag cggctcatgt actctcagga 721 catgctggct ggccatggcc gacttcagga aaacgggcga ttatctctgg aggaagtaca 781 atggggccat ccaggtggtc atgaaccagg atggcacagg tttcactgtg gctaacgaga 841 ggtttaagaa gccaacgaaa aatgacctcg tgtattttga gaattctcca gactactgta 901 tcagggaccg agaggcaggc tccctgggta cagcaggccg tgtgtgcaac ctgacttccc 961 ggggcatgga cagctgtgaa gtcatgtgct gtgggagagg ctacgacacc tcccatgtca 1021 cccggatgac caagtgtggg tgtaagttcc actggtgctg cgccgtgcgc tgtcaggact 1081 gcctggaagc tctggatgtg cacacatgca aggcccccaa gaacgctgac tggacaaccg 1141 ctacatgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1201 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1261 acctctggat ta