Construct: ORF ccsbBroad304_01837
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017203.1_s304c1, BRDN0000409030
- Derived from:
- ccsbBroadEn_01837
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEMA3B (7869)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01837
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7869 | SEMA3B | semaphorin 3B | NM_001005914.2 | 100% | 100% | |
| 2 | human | 7869 | SEMA3B | semaphorin 3B | NM_001290060.2 | 99.8% | 99.8% | 990_992delCAG |
| 3 | human | 7869 | SEMA3B | semaphorin 3B | NM_004636.3 | 99.8% | 99.8% | 990_992delCAG |
| 4 | human | 7869 | SEMA3B | semaphorin 3B | NM_001290061.1 | 99.2% | 99% | 923_937del;1005_1007delCAG |
| 5 | human | 7869 | SEMA3B | semaphorin 3B | NM_001290062.1 | 54.2% | 54.2% | 0_1ins1026 |
| 6 | human | 7869 | SEMA3B | semaphorin 3B | NM_001290063.1 | 54.2% | 54.2% | 0_1ins1026 |
| 7 | human | 7869 | SEMA3B | semaphorin 3B | NR_110697.1 | 43.9% | (many diffs) | |
| 8 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | NM_001042779.2 | 83.3% | 88.6% | (many diffs) |
| 9 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | NM_001291537.1 | 83.3% | 88.6% | (many diffs) |
| 10 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | NM_001291538.1 | 83.3% | 88.6% | (many diffs) |
| 11 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | NM_001291539.1 | 83.3% | 88.6% | (many diffs) |
| 12 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | NM_009153.3 | 83.3% | 88.6% | (many diffs) |
| 13 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_006511664.3 | 83.3% | 88.6% | (many diffs) |
| 14 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_006511665.3 | 83.3% | 88.6% | (many diffs) |
| 15 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_006511666.3 | 83.3% | 88.6% | (many diffs) |
| 16 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_006511671.3 | 83.3% | 88.6% | (many diffs) |
| 17 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_011242862.2 | 83.3% | 88.6% | (many diffs) |
| 18 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_011242863.2 | 83.3% | 88.6% | (many diffs) |
| 19 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_011242864.2 | 83.3% | 88.6% | (many diffs) |
| 20 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_011242865.2 | 83.3% | 88.6% | (many diffs) |
| 21 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_011242867.2 | 83.3% | 88.6% | (many diffs) |
| 22 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_017313225.1 | 83.3% | 88.6% | (many diffs) |
| 23 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_006511673.1 | 61.1% | 66.2% | (many diffs) |
| 24 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_006511674.3 | 59.8% | 60.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2310
- ORF length:
- 2244
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg gcgggccggg gctgccgccg tgatcccggg cctggccctg ctctgggcag 121 tggggctggg gagtgccgcc cccagccccc cacgccttcg gctctccttc caagagctcc 181 aggcctggca tggtctccag actttcagcc tggagcgaac ctgctgctac caggccttgc 241 tggtggatga ggagcgtgga cgcctgtttg tgggtgccga gaaccatgtg gcctccctca 301 acctggacaa catcagcaag cgggccaaga agctggcctg gccggcccct gtggaatggc 361 gagaggagtg caactgggca gggaaggaca ttggtactga gtgcatgaac ttcgtgaagt 421 tgctgcatgc ctacaaccgc acccatttgc tggcctgtgg cacgggagcc ttccacccaa 481 cctgtgcctt tgtggaagtg ggccaccggg cagaggagcc cgtcctccgg ctggacccag 541 gaaggataga ggatggcaag gggaagagtc cttatgaccc caggcatcgg gctgcctccg 601 tgctggtggg ggaggagcta tactcagggg tggcagcaga cctcatggga cgagacttta 661 ccatctttcg cagcctaggg caacgtccaa gtctccgaac agagccacac gactcccgct 721 ggctcaatga gcccaagttt gtcaaggtat tttggatccc ggagagcgag aacccagacg 781 acgacaaaat ctacttcttc tttcgtgaga cggcggtaga ggcggcgccg gcactgggac 841 gcctgtccgt gtcccgcgtt ggccagatct gccggaacga cgtgggcggc cagcgcagcc 901 tggtcaacaa gtggacgacg ttcctgaagg cgcggctggt gtgctcggtg cccggcgtcg 961 agggcgacac ccacttcgat cagctccagg atgtgtttct gttgtcctcg cgggaccacc 1021 ggaccccgct gctctatgcc gtcttctcca cgtccagcat cttccagggc tctgcggtgt 1081 gcgtgtacag catgaacgac gtgcgccggg ccttcttggg accctttgca cacaaggagg 1141 ggcccatgca ccagtgggtg tcataccagg gtcgcgtccc ctacccgcgg ccaggcatgt 1201 gccccagcaa gacctttggc accttcagtt ccaccaagga cttcccagac gatgtcatcc 1261 agtttgcgcg gaaccacccc ctcatgtaca actctgtcct gcccactggg gggcgccctc 1321 ttttcctaca agttggagcc aattacacct tcactcaaat tgccgcggac cgggttgcag 1381 ccgctgacgg acactatgac gtcctcttca ttggcacaga cgttggcacg gtgctgaagg 1441 tgatctcggt ccccaagggc agtaggccca gcgcagaggg gctgctcctg gaggagctgc 1501 acgtgtttga ggactcggcc gctgtcacca gcatgcaaat ttcttccaag aggcaccagc 1561 tgtacgtagc ctcgcggagc gcggtggccc agatcgcgtt gcaccgctgc gctgcccacg 1621 gccgcgtctg caccgaatgc tgtctggcgc gtgaccccta ctgcgcctgg gacggggtcg 1681 cgtgcacgcg cttccagccc agtgccaaga ggcggttccg gcggcaagac gtaaggaatg 1741 gcgaccccag cacgttgtgc tccggagact cgtctcgtcc cgcgctgctg gaacacaagg 1801 tgttcggcgt ggagggcagc agcgcctttc tggagtgtga gccccgctcg ctgcaggcgc 1861 gcgtggagtg gactttccag cgcgcagggg tgacagccca cacccaggtg ctggcagagg 1921 agcgcaccga gcgcaccgcc cggggactac tgctgcgcag gctgcggcgc cgggactcgg 1981 gcgtgtactt gtgcgccgcc gtcgagcagg gctttacgca accgctgcgt cgcctgtcgc 2041 tgcacgtgtt gagtgctacg caggccgaac gactggcgcg ggccgaggag gctgcgcccg 2101 ccgcgccgcc gggccccaaa ctctggtacc gggactttct gcagctggtg gagccgggcg 2161 gaggtggcag cgcgaactcc ctgcgcatgt gccgcccgca gcctgcgctg cagtcactgc 2221 ccctggagtc gcggagaaag ggccgtaacc ggaggaccca cgcccctgag cctcgcgctg 2281 agcgggggcc gcgcagcgca acgcactggt gcccaacttt cttgtacaaa gtggttggta 2341 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 2401 ggtggcgcgt taagtcgaca atcaacctct ggatta