Construct: ORF ccsbBroad304_01837

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017203.1_s304c1, BRDN0000409030
Derived from:
ccsbBroadEn_01837
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SEMA3B (7869)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01837
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7869 SEMA3B semaphorin 3B NM_001005914.2 100% 100%
2 human 7869 SEMA3B semaphorin 3B NM_001290060.2 99.8% 99.8% 990_992delCAG
3 human 7869 SEMA3B semaphorin 3B NM_004636.3 99.8% 99.8% 990_992delCAG
4 human 7869 SEMA3B semaphorin 3B NM_001290061.1 99.2% 99% 923_937del;1005_1007delCAG
5 human 7869 SEMA3B semaphorin 3B NM_001290062.1 54.2% 54.2% 0_1ins1026
6 human 7869 SEMA3B semaphorin 3B NM_001290063.1 54.2% 54.2% 0_1ins1026
7 human 7869 SEMA3B semaphorin 3B NR_110697.1 43.9% (many diffs)
8 mouse 20347 Sema3b sema domain, immunoglobulin... NM_001042779.2 83.3% 88.6% (many diffs)
9 mouse 20347 Sema3b sema domain, immunoglobulin... NM_001291537.1 83.3% 88.6% (many diffs)
10 mouse 20347 Sema3b sema domain, immunoglobulin... NM_001291538.1 83.3% 88.6% (many diffs)
11 mouse 20347 Sema3b sema domain, immunoglobulin... NM_001291539.1 83.3% 88.6% (many diffs)
12 mouse 20347 Sema3b sema domain, immunoglobulin... NM_009153.3 83.3% 88.6% (many diffs)
13 mouse 20347 Sema3b sema domain, immunoglobulin... XM_006511664.3 83.3% 88.6% (many diffs)
14 mouse 20347 Sema3b sema domain, immunoglobulin... XM_006511665.3 83.3% 88.6% (many diffs)
15 mouse 20347 Sema3b sema domain, immunoglobulin... XM_006511666.3 83.3% 88.6% (many diffs)
16 mouse 20347 Sema3b sema domain, immunoglobulin... XM_006511671.3 83.3% 88.6% (many diffs)
17 mouse 20347 Sema3b sema domain, immunoglobulin... XM_011242862.2 83.3% 88.6% (many diffs)
18 mouse 20347 Sema3b sema domain, immunoglobulin... XM_011242863.2 83.3% 88.6% (many diffs)
19 mouse 20347 Sema3b sema domain, immunoglobulin... XM_011242864.2 83.3% 88.6% (many diffs)
20 mouse 20347 Sema3b sema domain, immunoglobulin... XM_011242865.2 83.3% 88.6% (many diffs)
21 mouse 20347 Sema3b sema domain, immunoglobulin... XM_011242867.2 83.3% 88.6% (many diffs)
22 mouse 20347 Sema3b sema domain, immunoglobulin... XM_017313225.1 83.3% 88.6% (many diffs)
23 mouse 20347 Sema3b sema domain, immunoglobulin... XM_006511673.1 61.1% 66.2% (many diffs)
24 mouse 20347 Sema3b sema domain, immunoglobulin... XM_006511674.3 59.8% 60.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2310
ORF length:
2244
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg gcgggccggg gctgccgccg tgatcccggg cctggccctg ctctgggcag
121tggggctggg gagtgccgcc cccagccccc cacgccttcg gctctccttc caagagctcc
181aggcctggca tggtctccag actttcagcc tggagcgaac ctgctgctac caggccttgc
241tggtggatga ggagcgtgga cgcctgtttg tgggtgccga gaaccatgtg gcctccctca
301acctggacaa catcagcaag cgggccaaga agctggcctg gccggcccct gtggaatggc
361gagaggagtg caactgggca gggaaggaca ttggtactga gtgcatgaac ttcgtgaagt
421tgctgcatgc ctacaaccgc acccatttgc tggcctgtgg cacgggagcc ttccacccaa
481cctgtgcctt tgtggaagtg ggccaccggg cagaggagcc cgtcctccgg ctggacccag
541gaaggataga ggatggcaag gggaagagtc cttatgaccc caggcatcgg gctgcctccg
601tgctggtggg ggaggagcta tactcagggg tggcagcaga cctcatggga cgagacttta
661ccatctttcg cagcctaggg caacgtccaa gtctccgaac agagccacac gactcccgct
721ggctcaatga gcccaagttt gtcaaggtat tttggatccc ggagagcgag aacccagacg
781acgacaaaat ctacttcttc tttcgtgaga cggcggtaga ggcggcgccg gcactgggac
841gcctgtccgt gtcccgcgtt ggccagatct gccggaacga cgtgggcggc cagcgcagcc
901tggtcaacaa gtggacgacg ttcctgaagg cgcggctggt gtgctcggtg cccggcgtcg
961agggcgacac ccacttcgat cagctccagg atgtgtttct gttgtcctcg cgggaccacc
1021ggaccccgct gctctatgcc gtcttctcca cgtccagcat cttccagggc tctgcggtgt
1081gcgtgtacag catgaacgac gtgcgccggg ccttcttggg accctttgca cacaaggagg
1141ggcccatgca ccagtgggtg tcataccagg gtcgcgtccc ctacccgcgg ccaggcatgt
1201gccccagcaa gacctttggc accttcagtt ccaccaagga cttcccagac gatgtcatcc
1261agtttgcgcg gaaccacccc ctcatgtaca actctgtcct gcccactggg gggcgccctc
1321ttttcctaca agttggagcc aattacacct tcactcaaat tgccgcggac cgggttgcag
1381ccgctgacgg acactatgac gtcctcttca ttggcacaga cgttggcacg gtgctgaagg
1441tgatctcggt ccccaagggc agtaggccca gcgcagaggg gctgctcctg gaggagctgc
1501acgtgtttga ggactcggcc gctgtcacca gcatgcaaat ttcttccaag aggcaccagc
1561tgtacgtagc ctcgcggagc gcggtggccc agatcgcgtt gcaccgctgc gctgcccacg
1621gccgcgtctg caccgaatgc tgtctggcgc gtgaccccta ctgcgcctgg gacggggtcg
1681cgtgcacgcg cttccagccc agtgccaaga ggcggttccg gcggcaagac gtaaggaatg
1741gcgaccccag cacgttgtgc tccggagact cgtctcgtcc cgcgctgctg gaacacaagg
1801tgttcggcgt ggagggcagc agcgcctttc tggagtgtga gccccgctcg ctgcaggcgc
1861gcgtggagtg gactttccag cgcgcagggg tgacagccca cacccaggtg ctggcagagg
1921agcgcaccga gcgcaccgcc cggggactac tgctgcgcag gctgcggcgc cgggactcgg
1981gcgtgtactt gtgcgccgcc gtcgagcagg gctttacgca accgctgcgt cgcctgtcgc
2041tgcacgtgtt gagtgctacg caggccgaac gactggcgcg ggccgaggag gctgcgcccg
2101ccgcgccgcc gggccccaaa ctctggtacc gggactttct gcagctggtg gagccgggcg
2161gaggtggcag cgcgaactcc ctgcgcatgt gccgcccgca gcctgcgctg cagtcactgc
2221ccctggagtc gcggagaaag ggccgtaacc ggaggaccca cgcccctgag cctcgcgctg
2281agcgggggcc gcgcagcgca acgcactggt gcccaacttt cttgtacaaa gtggttggta
2341agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
2401ggtggcgcgt taagtcgaca atcaacctct ggatta

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