Construct: ORF ccsbBroad304_01907
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008801.1_s304c1, BRDN0000409696
- Derived from:
- ccsbBroadEn_01907
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H3C8 (8355)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01907
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8355 | H3C8 | H3 clustered histone 8 | NM_003534.2 | 100% | 100% | |
| 2 | human | 8351 | H3C4 | H3 clustered histone 4 | NM_003530.4 | 88.2% | 100% | (many diffs) |
| 3 | human | 8353 | H3C6 | H3 clustered histone 6 | NM_003532.2 | 86.7% | 100% | (many diffs) |
| 4 | human | 8357 | H3C10 | H3 clustered histone 10 | NM_003536.2 | 86.5% | 100% | (many diffs) |
| 5 | human | 8358 | H3C2 | H3 clustered histone 2 | NM_003537.3 | 86.5% | 100% | (many diffs) |
| 6 | human | 8968 | H3C7 | H3 clustered histone 7 | NM_021018.2 | 86.2% | 100% | (many diffs) |
| 7 | human | 8354 | H3C11 | H3 clustered histone 11 | NM_003533.2 | 85.7% | 100% | (many diffs) |
| 8 | human | 8290 | H3-4 | H3.4 histone | NM_003493.2 | 85.5% | 97% | (many diffs) |
| 9 | human | 8350 | H3C1 | H3 clustered histone 1 | NM_003529.2 | 85.2% | 100% | (many diffs) |
| 10 | human | 653604 | H3C13 | H3 clustered histone 13 | NM_001123375.2 | 84.5% | 99.2% | (many diffs) |
| 11 | human | 440686 | H3-2 | H3.2 histone (putative) | NM_001355409.3 | 84.1% | 97% | (many diffs) |
| 12 | human | 440686 | H3-2 | H3.2 histone (putative) | NM_001372105.1 | 84.1% | 97% | (many diffs) |
| 13 | human | 8352 | H3C3 | H3 clustered histone 3 | NM_003531.2 | 83.5% | 100% | (many diffs) |
| 14 | human | 333932 | H3C15 | H3 clustered histone 15 | NM_001005464.2 | 83.4% | 99.2% | (many diffs) |
| 15 | human | 126961 | H3C14 | H3 clustered histone 14 | NM_021059.2 | 83.4% | 99.2% | (many diffs) |
| 16 | human | 8356 | H3C12 | H3 clustered histone 12 | NM_003535.2 | 82.5% | 100% | (many diffs) |
| 17 | human | 3021 | H3-3B | H3.3 histone B | NM_005324.5 | 80.8% | 96.3% | (many diffs) |
| 18 | human | 440093 | H3-5 | H3.5 histone | NM_001013699.3 | 79.1% | 93.3% | (many diffs) |
| 19 | human | 3020 | H3-3A | H3.3 histone A | NM_002107.6 | 76.6% | 96.3% | (many diffs) |
| 20 | mouse | 319150 | Hist1h3b | histone cluster 1, H3b | NM_178203.2 | 87.2% | 99.2% | (many diffs) |
| 21 | mouse | 319149 | Hist1h3d | histone cluster 1, H3d | NM_178204.2 | 87.2% | 99.2% | (many diffs) |
| 22 | mouse | 319153 | Hist1h3i | histone cluster 1, H3i | NM_178207.2 | 87% | 100% | (many diffs) |
| 23 | mouse | 319152 | Hist1h3h | histone cluster 1, H3h | NM_178206.2 | 86.7% | 100% | (many diffs) |
| 24 | mouse | 360198 | Hist1h3a | histone cluster 1, H3a | NM_013550.5 | 86.2% | 100% | (many diffs) |
| 25 | mouse | 319148 | Hist1h3c | histone cluster 1, H3c | NM_175653.2 | 86.2% | 99.2% | (many diffs) |
| 26 | mouse | 97908 | Hist1h3g | histone cluster 1, H3g | NM_145073.2 | 86% | 100% | (many diffs) |
| 27 | mouse | 260423 | Hist1h3f | histone cluster 1, H3f | NM_013548.4 | 85.7% | 99.2% | (many diffs) |
| 28 | mouse | 97114 | Hist2h3c2 | histone cluster 2, H3c2 | NM_054045.4 | 85.7% | 99.2% | (many diffs) |
| 29 | mouse | 15077 | Hist2h3c1 | histone cluster 2, H3c1 | NM_178216.3 | 85.7% | 99.2% | (many diffs) |
| 30 | mouse | 319151 | Hist1h3e | histone cluster 1, H3e | NM_178205.2 | 85.5% | 99.2% | (many diffs) |
| 31 | mouse | 319154 | Hist2h3b | histone cluster 2, H3b | NM_178215.2 | 85.5% | 99.2% | (many diffs) |
| 32 | mouse | 382523 | Gm12260 | predicted gene 12260 | NM_001318003.2 | 82.5% | 97.7% | (many diffs) |
| 33 | mouse | 15081 | H3f3b | H3 histone, family 3B | NM_008211.3 | 80.5% | 96.3% | (many diffs) |
| 34 | mouse | 15081 | H3f3b | H3 histone, family 3B | XM_006532248.2 | 80.5% | 96.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 474
- ORF length:
- 408
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ccgcaccaag cagactgcac gcaagtccac cggtggcaaa gcgccgcgca 121 agcagctggc cactaaggcg gctcggaaaa gcgcgccggc caccggcggc gtgaagaaac 181 ctcatcgcta ccgtcccggc accgtggctc tgcgcgagat tcgccgctat cagaagtcga 241 ctgagctgct gatccgcaag ttgcctttcc aacgcctggt gcgagaaatc gctcaggact 301 tcaagacaga tctgcgcttt cagagttccg cggtgatggc cctgcaggag gcctgcgagg 361 cctacttggt ggggctcttt gaggatacca acctgtgtgc catccatgct aagcgagtga 421 ctatcatgcc caaggacatt cagctcgctc gccgcattcg tggggagaga gcgtacccaa 481 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 541 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta