Construct: ORF ccsbBroad304_01914
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001181.1_s304c1, BRDN0000404439
- Derived from:
- ccsbBroadEn_01914
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H4C2 (8366)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01914
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8366 | H4C2 | H4 clustered histone 2 | NM_003544.2 | 100% | 100% | |
| 2 | human | 8359 | H4C1 | H4 clustered histone 1 | NM_003538.3 | 84.7% | 100% | (many diffs) |
| 3 | human | 8294 | H4C9 | H4 clustered histone 9 | NM_003495.2 | 84.4% | 100% | (many diffs) |
| 4 | human | 8362 | H4C12 | H4 clustered histone 12 | NM_003541.2 | 84.1% | 100% | (many diffs) |
| 5 | human | 8367 | H4C5 | H4 clustered histone 5 | NM_003545.3 | 84.1% | 100% | (many diffs) |
| 6 | human | 8363 | H4C11 | H4 clustered histone 11 | NM_021968.3 | 84.1% | 100% | (many diffs) |
| 7 | human | 8364 | H4C3 | H4 clustered histone 3 | NM_003542.3 | 83.8% | 100% | (many diffs) |
| 8 | human | 8361 | H4C6 | H4 clustered histone 6 | NM_003540.3 | 83.4% | 100% | (many diffs) |
| 9 | human | 8365 | H4C8 | H4 clustered histone 8 | NM_003543.3 | 83.4% | 100% | (many diffs) |
| 10 | human | 8368 | H4C13 | H4 clustered histone 13 | NM_003546.2 | 82.2% | 100% | (many diffs) |
| 11 | human | 121504 | H4-16 | H4 histone 16 | NM_175054.2 | 82.2% | 100% | (many diffs) |
| 12 | human | 554313 | H4C15 | H4 clustered histone 15 | NM_001034077.4 | 81.2% | 100% | (many diffs) |
| 13 | human | 8370 | H4C14 | H4 clustered histone 14 | NM_003548.2 | 81.2% | 100% | (many diffs) |
| 14 | human | 8360 | H4C4 | H4 clustered histone 4 | NM_003539.3 | 80.5% | 100% | (many diffs) |
| 15 | mouse | 319158 | Hist1h4i | histone cluster 1, H4i | NM_175656.3 | 84.1% | 100% | (many diffs) |
| 16 | mouse | 100041230 | Hist1h4m | histone cluster 1, H4m | NM_001195421.1 | 83.8% | 100% | (many diffs) |
| 17 | mouse | 69386 | Hist1h4h | histone cluster 1, H4h | NM_153173.4 | 83.8% | 100% | (many diffs) |
| 18 | mouse | 319161 | Hist1h4n | histone cluster 1, H4n | NM_175657.2 | 83.8% | 100% | (many diffs) |
| 19 | mouse | 319156 | Hist1h4d | histone cluster 1, H4d | NM_175654.2 | 83.4% | 100% | (many diffs) |
| 20 | mouse | 326620 | Hist1h4b | histone cluster 1, H4b | NM_178193.2 | 82.8% | 100% | (many diffs) |
| 21 | mouse | 319160 | Hist1h4k | histone cluster 1, H4k | NM_178211.2 | 82.5% | 100% | (many diffs) |
| 22 | mouse | 319155 | Hist1h4c | histone cluster 1, H4c | NM_178208.2 | 82.2% | 100% | (many diffs) |
| 23 | mouse | 319157 | Hist1h4f | histone cluster 1, H4f | NM_175655.2 | 82.2% | 100% | (many diffs) |
| 24 | mouse | 326619 | Hist1h4a | histone cluster 1, H4a | NM_178192.2 | 82.2% | 100% | (many diffs) |
| 25 | mouse | 319159 | Hist1h4j | histone cluster 1, H4j | NM_178210.2 | 81.8% | 100% | (many diffs) |
| 26 | mouse | 320332 | Hist4h4 | histone cluster 4, H4 | NM_175652.3 | 81.2% | 100% | (many diffs) |
| 27 | mouse | 97122 | Hist2h4 | histone cluster 2, H4 | NM_033596.3 | 80.9% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 375
- ORF length:
- 309
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc tggtcgcggc aaaggcggta aaggtttggg taagggaggt gccaagcgtc 121 accgaaaagt gctgcgggat aacatccaag gcatcaccaa accggccatt cggcgccttg 181 ctaggcgtgg tggggttaag cgaatttccg gtttgattta tgaggagact cgtggcgttc 241 tcaaggtgtt tctggagaac gtgatccggg acgccgtgac ctacacggag cacgccaagc 301 gcaagactgt cactgccatg gatgtggttt acgcgctcaa gcgtcaagga cgcactctgt 361 acggcttcgg cggttaccca actttcttgt acaaagtggt tggtaagcct atccctaacc 421 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 481 cgacaatcaa cctctggatt a