Construct: ORF ccsbBroad304_01922
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004873.1_s304c1, BRDN0000407916
- Derived from:
- ccsbBroadEn_01922
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRPX (8406)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01922
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8406 | SRPX | sushi repeat containing pro... | NM_006307.5 | 100% | 100% | |
| 2 | human | 8406 | SRPX | sushi repeat containing pro... | NM_001170750.1 | 95.6% | 95.6% | 95_96ins60 |
| 3 | human | 8406 | SRPX | sushi repeat containing pro... | XM_017029893.2 | 89% | 87.5% | (many diffs) |
| 4 | human | 8406 | SRPX | sushi repeat containing pro... | NM_001170751.1 | 87.2% | 87% | 349_350ins177 |
| 5 | human | 8406 | SRPX | sushi repeat containing pro... | NM_001170752.1 | 81.6% | 79.5% | 1086_1087ins122;1137_1138ins133 |
| 6 | mouse | 51795 | Srpx | sushi-repeat-containing pro... | NM_016911.4 | 87.8% | 92.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1458
- ORF length:
- 1392
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg gagccccgca catcggcccg cgctgctgct gctgctgccg cctctgctgc 121 tgctgctgct gctgcgcgtc ccgcccagcc gcagcttccc aggatcggga gactcaccac 181 tagaagacga tgaagtcggg tattcacacc ctagatataa agataccccg tggtgctccc 241 ccatcaaggt gaagtatggg gatgtgtact gcagggcccc tcaaggagga tactacaaaa 301 cagccctggg aaccaggtgc gacattcgct gccagaaggg ctacgagctg catggctctt 361 ccctactgat ctgccagtca aacaaacgat ggtctgacaa ggtcatctgc aaacaaaagc 421 gatgtcctac ccttgccatg ccagcaaatg gagggtttaa gtgtgtagat ggtgcctact 481 ttaactcccg gtgtgagtat tattgttcac caggatacac gttgaaaggg gagcggaccg 541 tcacatgtat ggacaacaag gcctggagcg gccggccagc ctcctgtgtg gatatggaac 601 ctcctagaat caagtgccca agtgtgaagg aacgcattgc agaacccaac aaactgacag 661 tccgggtgtc ctgggagaca cccgaaggaa gagacacagc agatggaatt cttactgatg 721 tcattctaaa aggcctcccc ccaggctcca actttccaga aggagaccac aagatccagt 781 acacagtcta tgacagagct gagaataagg gcacttgcaa atttcgagtt aaagtaaGAG 841 TCAAACGCTG TGGCAAACTC AATGCCCCAG AGAATGGTTA CATGAAGTGC TCCAGCGACG 901 GTGATAATTA TGGAGCCACC TGTGAGTTCT CCTGCATCGG CGGCTATGAG CTCCAGGGTA 961 GCCCTGCCCG AGTATGTCAA TCCAACCTGG CTTGGTCTGG CACGGAGCCC ACCTGTGCAG 1021 CCATGAACGT CAATGTGGGT GTCAGAACGG CAGCTGCACT TCTGGATCAG TTTTATGAGA 1081 AAAGGAGACT CCTCATTGTG TCCACACCCA CAGCCCGAAA CCTCCTTTAC CGGCTCCAGC 1141 TAGGAATGCT GCAGCAAGCA CAGTGTGGCC TTGATCTTCG ACACATCACC GTGGTGGAGC 1201 TGGTGGGTGT GTTCCCGACT CTCATTGGCA GGATAGGAGC AAAGATTATG CCTCCAGCCC 1261 TAGCGCTGCA GCTCAGGCTG TTGCTGCGAA TCCCACTCTA CTCCTTCAGT ATGGTGCTAG 1321 TGGATAAGCA TGGCATGGAC AAAGAGCGCT ATGTCTCCCT GGTGATGCCT GTGGCCCTGT 1381 TCAACCTGAT TGACACTTTT CCCTTGAGAA AAGAAGAGAT GGTCCTACAA GCCGAAATGA 1441 GCCAGACCTG TAACACCTGC CCAACTTTCT TGTACaaagt ggttggtaag cctatcccta 1501 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 1561 agtcgacaat caacctctgg atta