Construct: ORF ccsbBroad304_02348
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011694.1_s304c1, BRDN0000399715
- Derived from:
- ccsbBroadEn_02348
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSME3 (10197)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02348
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10197 | PSME3 | proteasome activator subunit 3 | NM_005789.4 | 100% | 100% | |
| 2 | human | 10197 | PSME3 | proteasome activator subunit 3 | NM_176863.3 | 95.1% | 95.1% | 405_443del |
| 3 | human | 10197 | PSME3 | proteasome activator subunit 3 | NM_001267045.2 | 93.6% | 89.6% | (many diffs) |
| 4 | human | 10197 | PSME3 | proteasome activator subunit 3 | NM_001330229.2 | 75.9% | 75.9% | 0_1ins183 |
| 5 | human | 10197 | PSME3 | proteasome activator subunit 3 | NR_049772.2 | 20.3% | 1_235del;526_527ins113;885_3069del | |
| 6 | mouse | 19192 | Psme3 | proteaseome (prosome, macro... | NM_011192.3 | 94% | 100% | (many diffs) |
| 7 | mouse | 19192 | Psme3 | proteaseome (prosome, macro... | XM_006532525.3 | 87% | 91.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 828
- ORF length:
- 762
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ctcgttgctg aaggtggatc aggaagtgaa gctcaaggtt gattctttca 121 gggagcggat cacaagtgag gcagaagact tggtggcaaa ttttttccca aagaagttat 181 tagaacttga tagttttctg aaggaaccaa tcttaaacat ccatgaccta actcagatcc 241 actctgacat gaatctccca gtccctgacc ccattcttct caccaatagc catgatggac 301 tggatggtcc cacttataag aagcgaaggt tggatgagtg tgaagaagcc ttccaaggaa 361 ccaaggtgtt tgtgatgccc aatgggatgc tgaaaagcaa ccagcagctg gtggacatta 421 ttgagaaagt gaaacctgag atccggctgt tgattgagaa atgtaacacg gtcaaaatgt 481 gggtacagct cctgattccc aggatagaag atggaaacaa ctttggggtg tccattcagg 541 aggaaacagt tgcagagcta agaactgttg agagtgaagc tgcatcttat ctggaccaga 601 tttctagata ttatattaca agagccaaat tggtttctaa aatagctaaa tatccccatg 661 tggaggacta tcgccgcacc gtgacagaga ttgatgagaa agaatatatc agccttcggc 721 tcatcatatc agagctgagg aatcaatatg tcactctaca tgacatgatc ctgaaaaata 781 tcgagaagat caaacggccc cggagcagca atgcagagac tctgtactgc ccaactttct 841 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 901 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta