Construct: ORF ccsbBroad304_02448
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010580.1_s304c1, BRDN0000399370
- Derived from:
- ccsbBroadEn_02448
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE2E3 (10477)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02448
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10477 | UBE2E3 | ubiquitin conjugating enzym... | NM_001278554.1 | 100% | 100% | |
| 2 | human | 10477 | UBE2E3 | ubiquitin conjugating enzym... | NM_001278555.1 | 100% | 100% | |
| 3 | human | 10477 | UBE2E3 | ubiquitin conjugating enzym... | NM_006357.4 | 100% | 100% | |
| 4 | human | 10477 | UBE2E3 | ubiquitin conjugating enzym... | NM_182678.2 | 100% | 100% | |
| 5 | human | 10477 | UBE2E3 | ubiquitin conjugating enzym... | XM_005246244.2 | 100% | 100% | |
| 6 | human | 7325 | UBE2E2 | ubiquitin conjugating enzym... | NM_001370225.1 | 76.8% | 85.9% | (many diffs) |
| 7 | human | 7325 | UBE2E2 | ubiquitin conjugating enzym... | NM_152653.4 | 76.8% | 85.9% | (many diffs) |
| 8 | human | 10477 | UBE2E3 | ubiquitin conjugating enzym... | XM_017003166.2 | 54.6% | 40.6% | (many diffs) |
| 9 | human | 10477 | UBE2E3 | ubiquitin conjugating enzym... | XM_011510492.3 | 53.5% | 45.4% | (many diffs) |
| 10 | human | 286480 | UBE2E4P | ubiquitin conjugating enzym... | NR_110506.1 | 52.6% | (many diffs) | |
| 11 | human | 10477 | UBE2E3 | ubiquitin conjugating enzym... | XM_017003167.2 | 46.6% | 38.8% | (many diffs) |
| 12 | mouse | 22193 | Ube2e3 | ubiquitin-conjugating enzym... | NM_009454.2 | 95.4% | 100% | (many diffs) |
| 13 | mouse | 22193 | Ube2e3 | ubiquitin-conjugating enzym... | XM_006499161.3 | 95.4% | 100% | (many diffs) |
| 14 | mouse | 22193 | Ube2e3 | ubiquitin-conjugating enzym... | XM_006499162.3 | 95.4% | 100% | (many diffs) |
| 15 | mouse | 22193 | Ube2e3 | ubiquitin-conjugating enzym... | XM_006499163.3 | 95.4% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 687
- ORF length:
- 621
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc cagtgatagg caaaggtccg atgatgagag ccccagcacc agcagtggca 121 gttcagatgc ggaccagcga gacccagccg ctccagagcc tgaagaacaa gaggaaagaa 181 aaccttctgc cacccagcag aagaaaaaca ccaaactctc tagcaaaacc actgctaagt 241 tatccactag tgctaaaaga attcagaagg agctagctga aataaccctt gatcctcctc 301 ctaattgcag tgctgggcct aaaggagata acatttatga atggagatca actatacttg 361 gtccaccggg ttctgtatat gaaggtggtg tgttttttct ggatatcaca ttttcatcag 421 attatccatt taagccacca aaggttactt tccgcaccag aatctatcac tgcaacatca 481 acagtcaggg agtcatctgt ctggacatcc ttaaagacaa ctggagtccc gctttgacta 541 tttcaaaggt tttgctgtct atttgttccc ttttgacaga ctgcaaccct gcggatcctc 601 tggttggaag catagccact cagtatttga ccaacagagc agaacacgac aggatagcca 661 gacagtggac caagagatac gcaacatacc caactttctt gtacaaagtg gttggtaagc 721 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 781 ggcgcgttaa gtcgacaatc aacctctgga tta