Transcript: Human XM_005246244.2

PREDICTED: Homo sapiens ubiquitin conjugating enzyme E2 E3 (UBE2E3), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
UBE2E3 (10477)
Length:
1538
CDS:
376..999

Additional Resources:

NCBI RefSeq record:
XM_005246244.2
NBCI Gene record:
UBE2E3 (10477)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005246244.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000007578 CCACCGGGTTCTGTATATGAA pLKO.1 673 CDS 100% 5.625 3.375 N UBE2E3 n/a
2 TRCN0000318606 CCACCGGGTTCTGTATATGAA pLKO_005 673 CDS 100% 5.625 3.375 N UBE2E3 n/a
3 TRCN0000007576 GCTTTGACTATTTCAAAGGTT pLKO.1 841 CDS 100% 0.300 0.180 N UBE2E3 n/a
4 TRCN0000221009 CCCTTGATCCTCCTCCTAATT pLKO.1 596 CDS 100% 13.200 6.600 Y UBE2E4P n/a
5 TRCN0000007575 CGGACTTCTGTGTATATGTTA pLKO.1 1079 3UTR 100% 5.625 2.813 Y UBE2E3 n/a
6 TRCN0000318608 CGGACTTCTGTGTATATGTTA pLKO_005 1079 3UTR 100% 5.625 2.813 Y UBE2E3 n/a
7 TRCN0000221011 CTGAAGAACAAGAGGAAAGAA pLKO.1 470 CDS 100% 5.625 2.813 Y UBE2E4P n/a
8 TRCN0000040794 TGGGCCTAAAGGAGATAACAT pLKO.1 624 CDS 100% 5.625 2.813 Y Ube2e3 n/a
9 TRCN0000007579 AGAGCAGAACACGACAGGATA pLKO.1 946 CDS 100% 4.950 2.475 Y UBE2E3 n/a
10 TRCN0000318680 AGAGCAGAACACGACAGGATA pLKO_005 946 CDS 100% 4.950 2.475 Y UBE2E3 n/a
11 TRCN0000007577 CCACTGCTAAGTTATCCACTA pLKO.1 539 CDS 100% 4.050 2.025 Y UBE2E3 n/a
12 TRCN0000318678 CCACTGCTAAGTTATCCACTA pLKO_005 539 CDS 100% 4.050 2.025 Y UBE2E3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005246244.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02448 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02448 pLX_304 0% 100% 100% V5 n/a
3 ccsbBroadEn_01737 pDONR223 100% 76.8% 85.9% None (many diffs) n/a
4 ccsbBroad304_01737 pLX_304 0% 76.8% 85.9% V5 (many diffs) n/a
5 TRCN0000491351 TATATGCCAGTGTTGGAGCAGTAC pLX_317 41.4% 76.8% 85.9% V5 (many diffs) n/a
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