Transcript: Mouse XM_006499161.3

PREDICTED: Mus musculus ubiquitin-conjugating enzyme E2E 3 (Ube2e3), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ube2e3 (22193)
Length:
2221
CDS:
365..988

Additional Resources:

NCBI RefSeq record:
XM_006499161.3
NBCI Gene record:
Ube2e3 (22193)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006499161.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000007576 GCTTTGACTATTTCAAAGGTT pLKO.1 830 CDS 100% 0.300 0.420 N UBE2E3 n/a
2 TRCN0000007575 CGGACTTCTGTGTATATGTTA pLKO.1 1068 3UTR 100% 5.625 4.500 N UBE2E3 n/a
3 TRCN0000318608 CGGACTTCTGTGTATATGTTA pLKO_005 1068 3UTR 100% 5.625 4.500 N UBE2E3 n/a
4 TRCN0000040794 TGGGCCTAAAGGAGATAACAT pLKO.1 613 CDS 100% 5.625 3.938 N Ube2e3 n/a
5 TRCN0000040797 CGGAAGCATAGCCACTCAGTA pLKO.1 904 CDS 100% 4.950 3.465 N Ube2e3 n/a
6 TRCN0000040796 AGGTTACTTTCCGTACCAGAA pLKO.1 741 CDS 100% 4.050 2.835 N Ube2e3 n/a
7 TRCN0000040795 AGTGGCAGTTCAGATGCAGAT pLKO.1 413 CDS 100% 4.050 2.835 N Ube2e3 n/a
8 TRCN0000040793 CCTGGATATTCTGAAAGACAA pLKO.1 799 CDS 100% 4.950 2.970 N Ube2e3 n/a
9 TRCN0000221009 CCCTTGATCCTCCTCCTAATT pLKO.1 585 CDS 100% 13.200 9.240 N UBE2E4P n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006499161.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02448 pDONR223 100% 95.4% 100% None (many diffs) n/a
2 ccsbBroad304_02448 pLX_304 0% 95.4% 100% V5 (many diffs) n/a
3 ccsbBroadEn_01737 pDONR223 100% 77.1% 85.9% None (many diffs) n/a
4 ccsbBroad304_01737 pLX_304 0% 77.1% 85.9% V5 (many diffs) n/a
5 TRCN0000491351 TATATGCCAGTGTTGGAGCAGTAC pLX_317 41.4% 77.1% 85.9% V5 (many diffs) n/a
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