Construct: ORF ccsbBroad304_02661
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016753.1_s304c1, BRDN0000399190
- Derived from:
- ccsbBroadEn_02661
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FILIP1L (11259)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02661
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11259 | FILIP1L | filamin A interacting prote... | NM_001370247.1 | 100% | 100% | |
| 2 | human | 11259 | FILIP1L | filamin A interacting prote... | NM_014890.3 | 100% | 100% | |
| 3 | human | 11259 | FILIP1L | filamin A interacting prote... | NM_001282794.1 | 99.1% | 99.2% | (many diffs) |
| 4 | human | 11259 | FILIP1L | filamin A interacting prote... | XM_011512371.2 | 94.4% | 94.4% | 1_156del |
| 5 | human | 11259 | FILIP1L | filamin A interacting prote... | NM_001042459.2 | 78.8% | 78.8% | 1_720del |
| 6 | human | 11259 | FILIP1L | filamin A interacting prote... | NM_001282793.1 | 78.6% | 78.6% | (many diffs) |
| 7 | human | 11259 | FILIP1L | filamin A interacting prote... | NM_182909.3 | 78.2% | 78.2% | (many diffs) |
| 8 | mouse | 78749 | Filip1l | filamin A interacting prote... | NM_001177871.1 | 89.2% | 90.9% | (many diffs) |
| 9 | mouse | 78749 | Filip1l | filamin A interacting prote... | NM_001040397.4 | 70.5% | 71.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2745
- ORF length:
- 2679
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt ggtggatgaa cagcaaaggc tgacggcaca gctcaccctt caaagacaga 121 aaatccaaga gctgaccaca aatgcaaagg aaacacatac caaactagcc cttgctgaag 181 ccagagttca ggaggaagag cagaaggcaa ccagactaga gaaggaactg caaacgcaga 241 ccacaaagtt tcaccaagac caagacacaa ttatggcgaa gctcaccaat gaggacagtc 301 aaaatcgcca gcttcaacaa aagctggcag cactcagccg gcagattgat gagttagaag 361 agacaaacag gtctttacga aaagcagaag aggagctgca agatataaaa gaaaaaatca 421 gtaagggaga atatggaaac gctggtatca tggctgaagt ggaagagctc aggaaacgtg 481 tgctagatat ggaagggaaa gatgaagagc tcataaaaat ggaggagcag tgcagagatc 541 tcaataagag gcttgaaagg gagacgttac agagtaaaga ctttaaacta gaggttgaaa 601 aactcagtaa aagaattatg gctctggaaa agttagaaga cgctttcaac aaaagcaaac 661 aagaatgcta ctctctgaaa tgcaatttag aaaaagaaag gatgaccaca aagcagttgt 721 ctcaagaact ggagagttta aaagtaagga tcaaagagct agaagccatt gaaagtcggc 781 tagaaaagac agaattcact ctaaaagagg atttaactaa actgaaaaca ttaactgtga 841 tgtttgtaga tgaacggaaa acaatgagtg aaaaattaaa gaaaactgaa gataaattac 901 aagctgcttc ttctcagctt caagtggagc aaaataaagt aacaacagtt actgagaagt 961 taattgagga aactaaaagg gcgctcaagt ccaaaaccga tgtagaagaa aagatgtaca 1021 gcgtaaccaa ggagagagat gatttaaaaa acaaattgaa agcggaagaa gagaaaggaa 1081 atgatctcct gtcaagagtt aatatgttga aaaataggct tcaatcattg gaagcaattg 1141 agaaagattt cctaaaaaac aaattaaatc aagactctgg gaaatccaca acagcattac 1201 accaagaaaa caataagatt aaggagctct ctcaagaagt ggaaagactg aaactgaagc 1261 taaaggacat gaaagccatt gaggatgacc tcatgaaaac agaagatgaa tatgagactc 1321 tagaacgaag gtatgctaat gaacgagaca aagctcaatt tttatctaaa gagctagaac 1381 atgttaaaat ggaacttgct aagtacaagt tagcagaaaa gacagagacc agccatgaac 1441 aatggctttt caaaaggctt caagaagaag aagctaagtc agggcacctc tcaagagaag 1501 tggatgcatt aaaagagaaa attcatgaat acatggcaac tgaagaccta atatgtcacc 1561 tccagggaga tcactcagtc ctgcaaaaaa aactaaatca acaagaaaac aggaacagag 1621 atttaggaag agagattgaa aacctcacta aggagttaga gaggtaccgg catttcagta 1681 agagcctcag gcctagtctc aatggaagaa gaatttccga tcctcaagta ttttctaaag 1741 aagttcagac agaagcagta gacaatgaac cacctgatta caagagcctc attcctctgg 1801 aacgtgcagt catcaatggt cagttatatg aggagagtga gaatcaagac gaggacccta 1861 atgatgaggg atctgtgctg tccttcaaat gcagccagtc tactccatgt cctgttaaca 1921 gaaagctatg gattccctgg atgaaatcca aggagggcca tcttcagaat ggaaaaatgc 1981 aaactaaacc caatgccaac tttgtgcaac ctggagatct agtcctaagc cacacacctg 2041 ggcagccact tcatataaag gttactccag accatgtaca aaacacagcc actcttgaaa 2101 tcacaagtcc aaccacagag agtcctcact cttacacgag tactgcagtg ataccgaact 2161 gtggcacgcc aaagcaaagg ataaccatcc tccaaaacgc ctccataaca ccagtaaagt 2221 ccaaaacctc taccgaagac ctcatgaatt tagaacaagg catgtcccca attaccatgg 2281 caacctttgc cagagcacag accccagagt cttgtggttc tctaactcca gaaaggacaa 2341 tgtcccctat tcaggttttg gctgtgactg gttcagctag ctctcctgag cagggacgct 2401 ccccagaacc aacagaaatc agtgccaagc atgcgatatt cagagtctcc ccagaccggc 2461 agtcatcatg gcagtttcag cgttcaaaca gcaatagctc aagtgtgata actactgagg 2521 ataataaaat ccacattcac ttaggaagtc cttacatgca agctgtagcc agccctgtga 2581 gacctgccag cccttcagca ccactgcagg ataaccgaac tcaaggctta attaacgggg 2641 cactaaacaa aacaaccaat aaagtcacca gcagtattac tatcacacca acagccacac 2701 ctcttcctcg acaatcacaa attacagtaa gtaatatata taactgccca actttcttgt 2761 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 2821 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a