Construct: ORF ccsbBroad304_02709

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012970.1_s304c1, BRDN0000402491
Derived from:
ccsbBroadEn_02709
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SCMH1 (22955)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02709
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22955 SCMH1 Scm polycomb group protein ... NM_001031694.2 100% 100%
2 human 22955 SCMH1 Scm polycomb group protein ... XM_011541033.2 100% 100%
3 human 22955 SCMH1 Scm polycomb group protein ... XM_006710462.2 96% 93.9% (many diffs)
4 human 22955 SCMH1 Scm polycomb group protein ... XM_011541032.2 96% 93.9% (many diffs)
5 human 22955 SCMH1 Scm polycomb group protein ... XM_017000698.1 93.6% 93.6% 0_1ins60;1587_1588ins66
6 human 22955 SCMH1 Scm polycomb group protein ... XM_011541035.2 92.8% 92.7% 0_1ins141;2T>A
7 human 22955 SCMH1 Scm polycomb group protein ... NM_001172219.2 92.8% 90.7% (many diffs)
8 human 22955 SCMH1 Scm polycomb group protein ... NM_001172221.1 90.7% 90.7% 0_1ins183
9 human 22955 SCMH1 Scm polycomb group protein ... XM_006710464.1 90.7% 90.7% 0_1ins183
10 human 22955 SCMH1 Scm polycomb group protein ... XM_011541036.2 90.7% 90.7% 0_1ins183
11 human 22955 SCMH1 Scm polycomb group protein ... XM_017000699.1 90.7% 90.7% 0_1ins183
12 human 22955 SCMH1 Scm polycomb group protein ... NM_012236.4 89.4% 89.3% 0_1ins141;2T>A;1506_1507ins66
13 human 22955 SCMH1 Scm polycomb group protein ... NM_001172218.2 87.4% 87.4% 0_1ins183;1464_1465ins66
14 human 22955 SCMH1 Scm polycomb group protein ... NM_001172220.2 87.4% 87.4% 0_1ins183;1464_1465ins66
15 human 22955 SCMH1 Scm polycomb group protein ... XM_011541034.1 87% 79.5% (many diffs)
16 human 22955 SCMH1 Scm polycomb group protein ... NM_001350667.2 85.8% 81.4% 0_1ins277;106_109delGTGA
17 human 22955 SCMH1 Scm polycomb group protein ... XM_024454203.1 85.8% 81.4% 0_1ins277;106_109delGTGA
18 human 22955 SCMH1 Scm polycomb group protein ... XM_024454204.1 85.8% 81.4% 0_1ins277;106_109delGTGA
19 human 22955 SCMH1 Scm polycomb group protein ... XM_024454206.1 85.8% 81.4% 0_1ins277;106_109delGTGA
20 human 22955 SCMH1 Scm polycomb group protein ... XM_017000700.1 83.6% 77.9% (many diffs)
21 human 22955 SCMH1 Scm polycomb group protein ... XM_017000702.1 82.5% 78.1% 0_1ins277;106_109delGTGA;1374_1375ins66
22 human 22955 SCMH1 Scm polycomb group protein ... XM_017000703.1 82.5% 78.1% 0_1ins277;106_109delGTGA;1374_1375ins66
23 human 22955 SCMH1 Scm polycomb group protein ... XR_001737051.1 78.4% (many diffs)
24 human 22955 SCMH1 Scm polycomb group protein ... XM_011541039.1 78.3% 76.3% (many diffs)
25 human 22955 SCMH1 Scm polycomb group protein ... XR_946584.2 75.4% (many diffs)
26 human 22955 SCMH1 Scm polycomb group protein ... XM_011541043.2 74.5% 74.5% 0_1ins504
27 human 22955 SCMH1 Scm polycomb group protein ... XM_011541044.1 74.5% 74.5% 0_1ins504
28 human 22955 SCMH1 Scm polycomb group protein ... XM_011541040.2 73.4% 69.6% (many diffs)
29 human 22955 SCMH1 Scm polycomb group protein ... NM_001172222.2 72.6% 72.5% (many diffs)
30 human 22955 SCMH1 Scm polycomb group protein ... XM_017000708.1 72.5% 72.5% 0_1ins183;530_531ins360
31 human 22955 SCMH1 Scm polycomb group protein ... NM_001350668.1 71.2% 71.2% 0_1ins504;1143_1144ins66
32 human 22955 SCMH1 Scm polycomb group protein ... XM_017000711.1 69.2% 69.2% 0_1ins183;530_531ins360;1104_1105ins66
33 human 22955 SCMH1 Scm polycomb group protein ... XM_017000712.1 69.2% 69.2% 0_1ins183;530_531ins360;1104_1105ins66
34 human 22955 SCMH1 Scm polycomb group protein ... XR_002959760.1 67.9% (many diffs)
35 human 22955 SCMH1 Scm polycomb group protein ... XM_017000713.2 67.6% 63.4% 0_1ins277;106_109delGTGA;440_441ins360
36 human 22955 SCMH1 Scm polycomb group protein ... XR_002959763.1 67.6% (many diffs)
37 human 22955 SCMH1 Scm polycomb group protein ... XR_002959762.1 67.5% (many diffs)
38 human 22955 SCMH1 Scm polycomb group protein ... XR_002959761.1 65.2% (many diffs)
39 human 22955 SCMH1 Scm polycomb group protein ... XR_001737054.1 61.2% (many diffs)
40 human 22955 SCMH1 Scm polycomb group protein ... XR_001737045.1 59.4% (many diffs)
41 human 22955 SCMH1 Scm polycomb group protein ... NR_135092.1 58% 1_369del;2017_2070del;2404_3412del
42 human 22955 SCMH1 Scm polycomb group protein ... XR_002959757.1 56% (many diffs)
43 human 22955 SCMH1 Scm polycomb group protein ... XR_001737052.1 55% (many diffs)
44 human 22955 SCMH1 Scm polycomb group protein ... XR_001737053.1 54.9% (many diffs)
45 human 22955 SCMH1 Scm polycomb group protein ... XR_001737046.1 54.1% (many diffs)
46 mouse 29871 Scmh1 sex comb on midleg homolog 1 NM_013883.2 90.6% 93% (many diffs)
47 mouse 29871 Scmh1 sex comb on midleg homolog 1 XM_011240551.1 90.6% 93% (many diffs)
48 mouse 29871 Scmh1 sex comb on midleg homolog 1 XM_017320252.1 90.6% 93% (many diffs)
49 mouse 29871 Scmh1 sex comb on midleg homolog 1 XM_017320253.1 90.6% 93% (many diffs)
50 mouse 29871 Scmh1 sex comb on midleg homolog 1 XM_011240550.1 87.6% 86% (many diffs)
51 mouse 29871 Scmh1 sex comb on midleg homolog 1 XM_011240552.1 87% 89.4% (many diffs)
52 mouse 29871 Scmh1 sex comb on midleg homolog 1 XM_017320254.1 85.5% 87.8% (many diffs)
53 mouse 29871 Scmh1 sex comb on midleg homolog 1 NM_001159630.1 85.1% 87.1% (many diffs)
54 mouse 29871 Scmh1 sex comb on midleg homolog 1 XM_006503149.2 82.6% 80.9% (many diffs)
55 mouse 29871 Scmh1 sex comb on midleg homolog 1 XM_006503150.3 81.6% 84% (many diffs)
56 mouse 29871 Scmh1 sex comb on midleg homolog 1 XR_001784154.1 55.6% (many diffs)
57 mouse 29871 Scmh1 sex comb on midleg homolog 1 XR_001784153.1 54.4% (many diffs)
58 mouse 29871 Scmh1 sex comb on midleg homolog 1 XR_001784155.1 21.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2046
ORF length:
1980
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct ggtttgctac agtgttttag cttgtgagat tctctgggac cttccctgct
121ccatcatggg gtcacctcta ggtcatttta cctgggacaa atacctaaaa gaaacatgtt
181cagtcccagc gcctgtccat tgcttcaagc agtcctacac acctccaagc aacgagttca
241agatcagtat gaaattggaa gcacaggacc ccaggaacac cacatccacc tgtattgcca
301cagtagttgg actgacaggt gcccgccttc gcctgcgcct tgatgggagc gacaacaaaa
361atgacttctg gcggctggtt gactcagctg aaatccagcc tattgggaac tgtgaaaaga
421atgggggtat gctacagcca cctcttggat ttcggctgaa tgcgtcttct tggcccatgt
481tccttttgaa gacgctaaat ggagcagaga tggctcccat caggattttc cacaaggagc
541caccatcgcc ttcccacaac ttcttcaaaa tgggaatgaa gctagaagct gtggacagga
601agaaccctca tttcatttgc ccagccacta ttggggaggt tcggggctca gaggtgcttg
661tcacttttga tgggtggcga ggggcctttg actactggtg ccgcttcgac tcccgagaca
721tcttccctgt gggctggtgt tccttgactg gagacaacct gcagcctcct ggcaccaaag
781ttgtgattcc aaagaatccc tatcctgcct ccgatgtgaa tactgagaag cccagcatcc
841acagcagcac caaaactgtc ttggaacatc aaccagggca gagggggcgt aaaccaggaa
901agaagcgggg ccggacaccc aagaccctaa tttcccatcc catctctgcc ccatccaaga
961cagctgaacc tttgaaattc ccaaagaaga gaggtcccaa acctggcagc aagaggaaac
1021ctcggacttt gctgaaccca ccacctgcct caccaacaac cagcactcct gaaccggata
1081ccagcactgt accccaggat gctgccacca tccccagctc agccatgcag gccccaacag
1141tttgtatcta cttgaacaag aatggcagca caggccccca cttagataag aagaaggtcc
1201agcaactccc tgaccatttt ggaccagccc gtgcctctgt ggtgttgcag caggctgtcc
1261aggcctgtat cgactgtgct tatcaccaga aaaccgtctt cagcttcctc aagcaaggcc
1321atggtggtga ggttatctca gccgtgtttg accgggaaca gcataccctc aacctcccag
1381cagtcaacag catcacctac gtcctccgct tcctggagaa actctgccac aaccttcgta
1441gtgacaatct gtttggcaac cagcccttta cacagactca cttgtcactc actgccatag
1501agtacagcca cagccacgac aggtacctac caggtgaaac ctttgtcctg gggaatagtc
1561tggcccgctc cttggaacca cactcagact caatggactc tgcctcaaat cccaccaacc
1621ttgtcagcac ctcccaaagg caccggccct tgctttcatc ctgtggcctc ccaccaagca
1681ctgcctcagc tgtgcgcagg ctatgctcca ggggagtgtt aaaaggatca aatgaaagaa
1741gggatatgga atcattttgg aaactaaatc gttccccagg gtcggaccga tacctggaga
1801gccgcgatgc ctctcgactg agtggccggg acccctcctc atggacagtc gaggatgtga
1861tgcagtttgt ccgggaagct gatcctcagc ttggacccca cgctgacctg tttcgcaaac
1921acgagatcga tggcaaggcc ctgctgctgc tgcgcagtga catgatgatg aagtacatgg
1981gcctgaagct ggggcctgca ctcaagctct cctaccacat tgaccggctg aagcagggca
2041agttctgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
2101gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
2161acctctggat ta

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