Transcript: Human XM_024454204.1

PREDICTED: Homo sapiens Scm polycomb group protein homolog 1 (SCMH1), transcript variant X12, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SCMH1 (22955)
Length:
3211
CDS:
510..2219

Additional Resources:

NCBI RefSeq record:
XM_024454204.1
NBCI Gene record:
SCMH1 (22955)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024454204.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000416799 GGGCAAACCCTGTAGTTAATA pLKO_005 2694 3UTR 100% 15.000 21.000 N SCMH1 n/a
2 TRCN0000413985 ACAACCTTCGTAGTGACAATC pLKO_005 1600 CDS 100% 10.800 15.120 N SCMH1 n/a
3 TRCN0000424392 AGGTTATCTCAGCCGTGTTTG pLKO_005 1501 CDS 100% 10.800 15.120 N SCMH1 n/a
4 TRCN0000021810 GCCTGTATCGACTGTGCTTAT pLKO.1 1434 CDS 100% 10.800 15.120 N SCMH1 n/a
5 TRCN0000432404 GGACACCCAAGACCCTAATTT pLKO_005 1084 CDS 100% 15.000 10.500 N SCMH1 n/a
6 TRCN0000414895 AGTTCAAGATCAGTATGAAAT pLKO_005 402 5UTR 100% 13.200 9.240 N SCMH1 n/a
7 TRCN0000425850 CAATGGACTCTGCCTCAAATC pLKO_005 1762 CDS 100% 10.800 7.560 N SCMH1 n/a
8 TRCN0000440334 GTTTGGCAACCAGCCCTTTAC pLKO_005 1622 CDS 100% 10.800 7.560 N SCMH1 n/a
9 TRCN0000336312 TCATTTGCCCAGCCACTATTG pLKO_005 784 CDS 100% 10.800 7.560 N Scmh1 n/a
10 TRCN0000021809 CCCAACAGTTTGTATCTACTT pLKO.1 1304 CDS 100% 4.950 3.465 N SCMH1 n/a
11 TRCN0000021813 GAACACCACATCCACCTGTAT pLKO.1 442 5UTR 100% 4.950 3.465 N SCMH1 n/a
12 TRCN0000097996 GCGCAGTGACATGATGATGAA pLKO.1 2123 CDS 100% 4.950 3.465 N Scmh1 n/a
13 TRCN0000021812 GTCGAGGATGTGATGCAGTTT pLKO.1 2019 CDS 100% 4.950 3.465 N SCMH1 n/a
14 TRCN0000097998 CCCTTTACACAGACTCACTTA pLKO.1 1635 CDS 100% 4.950 3.465 N Scmh1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024454204.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02710 pDONR223 100% 94.5% 89.7% None 0_1ins94;106_109delGTGA n/a
2 ccsbBroad304_02710 pLX_304 0% 94.5% 89.7% V5 0_1ins94;106_109delGTGA n/a
3 ccsbBroadEn_02709 pDONR223 100% 85.8% 81.4% None 0_1ins277;106_109delGTGA n/a
4 ccsbBroad304_02709 pLX_304 0% 85.8% 81.4% V5 0_1ins277;106_109delGTGA n/a
5 TRCN0000469074 GTTCTTCCGAGACCTAACCCAAGT pLX_317 18.5% 85.8% 81.4% V5 0_1ins277;106_109delGTGA n/a
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