Construct: ORF ccsbBroad304_02759
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016769.1_s304c1, BRDN0000462426
- Derived from:
- ccsbBroadEn_02759
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DICER1 (23405)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02759
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23405 | DICER1 | dicer 1, ribonuclease III | NM_001271282.3 | 100% | 100% | |
| 2 | human | 23405 | DICER1 | dicer 1, ribonuclease III | NM_001291628.1 | 100% | 100% | |
| 3 | human | 23405 | DICER1 | dicer 1, ribonuclease III | NM_030621.4 | 100% | 100% | |
| 4 | human | 23405 | DICER1 | dicer 1, ribonuclease III | NM_177438.2 | 100% | 100% | |
| 5 | human | 23405 | DICER1 | dicer 1, ribonuclease III | XM_011536599.2 | 100% | 100% | |
| 6 | human | 23405 | DICER1 | dicer 1, ribonuclease III | XM_011536600.3 | 100% | 100% | |
| 7 | human | 23405 | DICER1 | dicer 1, ribonuclease III | XM_011536601.3 | 100% | 100% | |
| 8 | human | 23405 | DICER1 | dicer 1, ribonuclease III | XM_011536602.3 | 100% | 100% | |
| 9 | human | 23405 | DICER1 | dicer 1, ribonuclease III | XM_017021120.2 | 100% | 100% | |
| 10 | human | 23405 | DICER1 | dicer 1, ribonuclease III | XM_017021121.2 | 100% | 100% | |
| 11 | human | 23405 | DICER1 | dicer 1, ribonuclease III | NM_001195573.1 | 95.1% | 93.2% | 5362_5363ins163;5487_5488ins116 |
| 12 | human | 23405 | DICER1 | dicer 1, ribonuclease III | XM_011536604.2 | 92.9% | 92.5% | 0_1ins274;32_33ins131 |
| 13 | human | 23405 | DICER1 | dicer 1, ribonuclease III | XM_017021122.2 | 92.9% | 92.5% | 0_1ins274;32_33ins131 |
| 14 | human | 23405 | DICER1 | dicer 1, ribonuclease III | XM_017021123.2 | 92.9% | 92.5% | 0_1ins274;32_33ins131 |
| 15 | human | 23405 | DICER1 | dicer 1, ribonuclease III | XM_011536605.2 | 74.3% | 74.3% | 0_1ins1479 |
| 16 | mouse | 192119 | Dicer1 | dicer 1, ribonuclease type III | NM_148948.2 | 88.4% | 92.4% | (many diffs) |
| 17 | mouse | 192119 | Dicer1 | dicer 1, ribonuclease type III | XM_006515608.3 | 88.4% | 92.4% | (many diffs) |
| 18 | mouse | 192119 | Dicer1 | dicer 1, ribonuclease type III | XM_006515609.3 | 88.4% | 92.4% | (many diffs) |
| 19 | mouse | 192119 | Dicer1 | dicer 1, ribonuclease type III | XM_006515611.3 | 88.4% | 92.4% | (many diffs) |
| 20 | mouse | 192119 | Dicer1 | dicer 1, ribonuclease type III | XM_017314997.1 | 82.7% | 85.1% | (many diffs) |
| 21 | mouse | 192119 | Dicer1 | dicer 1, ribonuclease type III | XM_017314998.1 | 81.8% | 85.6% | (many diffs) |
| 22 | mouse | 192119 | Dicer1 | dicer 1, ribonuclease type III | XM_017314999.1 | 65.6% | 68.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 5835
- ORF length:
- 5766
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaaagccct gctttgcaac ccctcagcat ggcaggcctg cagctcatga 121 cccctgcttc ctcaccaatg ggtcctttct ttggactgcc atggcaacaa gaagcaattc 181 atgataacat ttatacgcca agaaaatatc aggttgaact gcttgaagca gctctggatc 241 ataataccat cgtctgttta aacactggct cagggaagac atttattgca gtactactca 301 ctaaagagct gtcctatcag atcaggggag acttcagcag aaatggaaaa aggacggtgt 361 tcttggtcaa ctctgcaaac caggttgctc aacaagtgtc agctgtcaga actcattcag 421 atctcaaggt tggggaatac tcaaacctag aagtaaatgc atcttggaca aaagagagat 481 ggaaccaaga gtttactaag caccaggttc tcattatgac ttgctatgtc gccttgaatg 541 ttttgaaaaa tggttactta tcactgtcag acattaacct tttggtgttt gatgagtgtc 601 atcttgcaat cctagaccac ccctatcgag aaattatgaa gctctgtgaa aattgtccat 661 catgtcctcg cattttggga ctaactgctt ccattttaaa tgggaaatgt gatccagagg 721 aattggaaga aaagattcag aaactagaga aaattcttaa gagtaatgct gaaactgcaa 781 ctgacctggt ggtcttagac aggtatactt ctcagccatg tgagattgtg gtggattgtg 841 gaccatttac tgacagaagt gggctttatg aaagactgct gatggaatta gaagaagcac 901 ttaattttat caatgattgt aatatatctg tacattcaaa agaaagagat tctactttaa 961 tttcgaaaca gatactatca gactgtcgtg ccgtattggt agttctggga ccctggtgtg 1021 cagataaagt agctggaatg atggtaagag aactacagaa atacatcaaa catgagcaag 1081 aggagctgca caggaaattt ttattgttta cagacacttt cctaaggaaa atacatgcac 1141 tatgtgaaga gcacttctca cctgcctcac ttgacctgaa atttgtaact cctaaagtaa 1201 tcaaactgct cgaaatctta cgcaaatata aaccatatga gcgacagcag tttgaaagcg 1261 ttgagtggta taataataga aatcaggata attatgtgtc atggagtgat tctgaggatg 1321 atgatgagga tgaagaaatt gaagaaaaag agaagccaga gacaaatttt ccttctcctt 1381 ttaccaacat tttgtgcgga attatttttg tggaaagaag atacacagca gttgtcttaa 1441 acagattgat aaaggaagct ggcaaacaag atccagagct ggcttatatc agtagcaatt 1501 tcataactgg acatggcatt gggaagaatc agcctcgcaa caaacagatg gaagcagaat 1561 tcagaaaaca ggaagaggta cttaggaaat ttcgagcaca tgagaccaac ctgcttattg 1621 caacaagtat tgtagaagag ggtgttgata taccaaaatg caacttggtg gttcgttttg 1681 atttgcccac agaatatcga tcctatgttc aatctaaagg aagagcaagg gcacccatct 1741 ctaattatat aatgttagcg gatacagaca aaataaaaag ttttgaagaa gaccttaaaa 1801 cctacaaagc tattgaaaag atcttgagaa acaagtgttc caagtcggtt gatactggtg 1861 agactgacat tgatcctgtc atggatgatg atgacgtttt cccaccatat gtgttgaggc 1921 ctgacgatgg tggtccacga gtcacaatca acacggccat tggacacatc aatagatact 1981 gtgctagatt accaagtgat ccgtttactc atctagctcc taaatgcaga acccgagagt 2041 tgcctgatgg tacattttat tcaactcttt atctgccaat taactcacct cttcgagcct 2101 ccattgttgg tccaccaatg agctgtgtac gattggctga aagagttgta gctctcattt 2161 gctgtgagaa actgcacaaa attggcgaac tggatgacca tttgatgcca gttgggaaag 2221 agactgttaa atatgaagag gagcttgatt tgcatgatga agaagagacc agtgttccag 2281 gaagaccagg ttccacgaaa cgaaggcagt gctacccaaa agcaattcca gagtgtttga 2341 gggatagtta tcccagacct gatcagccct gttacctgta tgtgatagga atggttttaa 2401 ctacaccttt acctgatgaa ctcaacttta gaaggcggaa gctctatcct cctgaagata 2461 ccacaagatg ctttggaata ctgacggcca aacccatacc tcagattcca cactttcctg 2521 tgtacacacg ctctggagag gttaccatat ccattgagtt gaagaagtct ggtttcatgt 2581 tgtctctaca aatgcttgag ttgattacaa gacttcacca gtatatattc tcacatattc 2641 ttcggcttga aaaacctgca ctagaattta aacctacaga cgctgattca gcatactgtg 2701 ttctacctct taatgttgtt aatgactcca gcactttgga tattgacttt aaattcatgg 2761 aagatattga gaagtctgaa gctcgcatag gcattcccag tacaaagtat acaaaagaaa 2821 caccctttgt ttttaaatta gaagattacc aagatgccgt tatcattcca agatatcgca 2881 attttgatca gcctcatcga ttttatgtag ctgatgtgta cactgatctt accccactca 2941 gtaaatttcc ttcccctgag tatgaaactt ttgcagaata ttataaaaca aagtacaacc 3001 ttgacctaac caatctcaac cagccactgc tggatgtgga ccacacatct tcaagactta 3061 atcttttgac acctcgacat ttgaatcaga aggggaaagc gcttccttta agcagtgctg 3121 agaagaggaa agccaaatgg gaaagtctgc agaataaaca gatactggtt ccagaactct 3181 gtgctataca tccaattcca gcatcactgt ggagaaaagc tgtttgtctc cccagcatac 3241 tttatcgcct tcactgcctt ttgactgcag aggagctaag agcccagact gccagcgatg 3301 ctggcgtggg agtcagatca cttcctgcgg attttagata ccctaactta gacttcgggt 3361 ggaaaaaatc tattgacagc aaatctttca tctcaatttc taactcctct tcagctgaaa 3421 atgataatta ctgtaagcac agcacaattg tccctgaaaa tgctgcacat caaggtgcta 3481 atagaacctc ctctctagaa aatcatgacc aaatgtctgt gaactgcaga acgttgctca 3541 gcgagtcccc tggtaagctc cacgttgaag tttcagcaga tcttacagca attaatggtc 3601 tttcttacaa tcaaaatctc gccaatggca gttatgattt agctaacaga gacttttgcc 3661 aaggaaatca gctaaattac tacaagcagg aaatacccgt gcaaccaact acctcatatt 3721 ccattcagaa tttatacagt tacgagaacc agccccagcc cagcgatgaa tgtactctcc 3781 tgagtaataa ataccttgat ggaaatgcta acaaatctac ctcagatgga agtcctgtga 3841 tggccgtaat gcctggtacg acagacacta ttcaagtgct caagggcagg atggattctg 3901 agcagagccc ttctattggg tactcctcaa ggactcttgg ccccaatcct ggacttattc 3961 ttcaggcttt gactctgtca aacgctagtg atggatttaa cctggagcgg cttgaaatgc 4021 ttggcgactc ctttttaaag catgccatca ccacatatct attttgcact taccctgatg 4081 cgcatgaggg ccgcctttca tatatgagaa gcaaaaaggt cagcaactgt aatctgtatc 4141 gccttggaaa aaagaaggga ctacccagcc gcatggtggt gtcaatattt gatccccctg 4201 tgaattggct tcctcctggt tatgtagtaa atcaagacaa aagcaacaca gataaatggg 4261 aaaaagatga aatgacaaaa gactgcatgc tggcgaatgg caaactggat gaggattacg 4321 aggaggagga tgaggaggag gagagcctga tgtggagggc tccgaaggaa gaggctgact 4381 atgaagatga tttcctggag tatgatcagg aacatatcag atttatagat aatatgttaa 4441 tggggtcagg agcttttgta aagaaaatct ctctttctcc tttttcaacc actgattctg 4501 catatgaatg gaaaatgccc aaaaaatcct ccttaggtag tatgccattt tcatcagatt 4561 ttgaggattt tgactacagc tcttgggatg caatgtgcta tctggatcct agcaaagctg 4621 ttgaagaaga tgactttgtg gtggggttct ggaatccatc agaagaaaac tgtggtgttg 4681 acacgggaaa gcagtccatt tcttacgact tgcacactga gcagtgtatt gctgacaaaa 4741 gcatagcgga ctgtgtggaa gccctgctgg gctgctattt aaccagctgt ggggagaggg 4801 ctgctcagct tttcctctgt tcactggggc tgaaggtgct cccggtaatt aaaaggactg 4861 atcgggaaaa ggccctgtgc cctactcggg agaatttcaa cagccaacaa aagaaccttt 4921 cagtgagctg tgctgctgct tctgtggcca gttcacgctc ttctgtattg aaagactcgg 4981 aatatggttg tttgaagatt ccaccaagat gtatgtttga tcatccagat gcagataaaa 5041 cactgaatca ccttatatcg gggtttgaaa attttgaaaa gaaaatcaac tacagattca 5101 agaataaggc ttaccttctc caggctttta cacatgcctc ctaccactac aatactatca 5161 ctgattgtta ccagcgctta gaattcctgg gagatgcgat tttggactac ctcataacca 5221 agcaccttta tgaagacccg cggcagcact ccccgggggt cctgacagac ctgcggtctg 5281 ccctggtcaa caacaccatc tttgcatcgc tggctgtaaa gtacgactac cacaagtact 5341 tcaaagctgt ctctcctgag ctcttccatg tcattgatga ctttgtgcag tttcagcttg 5401 agaagaatga aatgcaagga atggattctg agcttaggag atctgaggag gatgaagaga 5461 aagaagagga tattgaagtt ccaaaggcca tgggggatat ttttgagtcg cttgctggtg 5521 ccatttacat ggatagtggg atgtcactgg agacagtctg gcaggtgtac tatcccatga 5581 tgcggccact aatagaaaag ttttctgcaa atgtaccccg ttcccctgtg cgagaattgc 5641 ttgaaatgga accagaaact gccaaattta gcccggctga gagaacttac gacgggaagg 5701 tcagagtcac tgtggaagta gtaggaaagg ggaaatttaa aggtgttggt cgaagttaca 5761 ggattgccaa atctgcagca gcaagaagag ccctccgaag cctcaaagct aatcaacctc 5821 aggttcccaa tagcttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc 5881 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 5941 cgacaatcaa cctctggatt a