Construct: ORF ccsbBroad304_02913
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018322.1_s304c1, BRDN0000410639
- Derived from:
- ccsbBroadEn_02913
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HERC4 (26091)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02913
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26091 | HERC4 | HECT and RLD domain contain... | NM_015601.4 | 100% | 100% | |
| 2 | human | 26091 | HERC4 | HECT and RLD domain contain... | XM_024447931.1 | 100% | 100% | |
| 3 | human | 26091 | HERC4 | HECT and RLD domain contain... | NM_022079.3 | 99.2% | 99.2% | 1926_1949del |
| 4 | human | 26091 | HERC4 | HECT and RLD domain contain... | XM_024447929.1 | 99.2% | 99.2% | 1926_1949del |
| 5 | human | 26091 | HERC4 | HECT and RLD domain contain... | XM_024447930.1 | 99.2% | 99.2% | 1926_1949del |
| 6 | human | 26091 | HERC4 | HECT and RLD domain contain... | XM_011539593.3 | 97.7% | 97.7% | 384_455del |
| 7 | human | 26091 | HERC4 | HECT and RLD domain contain... | XM_011539592.3 | 97% | 97% | 384_455del;1998_2021del |
| 8 | human | 26091 | HERC4 | HECT and RLD domain contain... | XM_024447927.1 | 97% | 97% | 384_455del;1998_2021del |
| 9 | human | 26091 | HERC4 | HECT and RLD domain contain... | XM_024447928.1 | 97% | 97% | 384_455del;1998_2021del |
| 10 | human | 26091 | HERC4 | HECT and RLD domain contain... | XM_011539596.2 | 92.5% | 92.1% | (many diffs) |
| 11 | human | 26091 | HERC4 | HECT and RLD domain contain... | NM_001278185.2 | 91.8% | 91.8% | 1926_1949del;2360_2361ins234 |
| 12 | human | 26091 | HERC4 | HECT and RLD domain contain... | XM_011539594.2 | 90.5% | 90.1% | (many diffs) |
| 13 | human | 26091 | HERC4 | HECT and RLD domain contain... | XM_011539595.2 | 90.5% | 90.1% | (many diffs) |
| 14 | human | 26091 | HERC4 | HECT and RLD domain contain... | XM_017016041.2 | 87.7% | 87.7% | 1442_1443ins363;1563_1586del |
| 15 | human | 26091 | HERC4 | HECT and RLD domain contain... | XM_011539597.3 | 85.8% | 85.8% | 384_455del;1514_1515ins363;1635_1658del |
| 16 | human | 26091 | HERC4 | HECT and RLD domain contain... | NM_001278186.2 | 75.5% | 75.3% | (many diffs) |
| 17 | human | 26091 | HERC4 | HECT and RLD domain contain... | XR_001747081.2 | 53.3% | (many diffs) | |
| 18 | human | 26091 | HERC4 | HECT and RLD domain contain... | XM_024447932.1 | 50.5% | 50.5% | 0_1ins1545;381_404del |
| 19 | human | 26091 | HERC4 | HECT and RLD domain contain... | NM_001278187.2 | 9.3% | 7.5% | (many diffs) |
| 20 | human | 26091 | HERC4 | HECT and RLD domain contain... | XM_024447933.1 | 9.3% | 7.5% | (many diffs) |
| 21 | mouse | 67345 | Herc4 | hect domain and RLD 4 | NM_026101.4 | 91.6% | 96.9% | (many diffs) |
| 22 | mouse | 67345 | Herc4 | hect domain and RLD 4 | NM_030114.2 | 90.9% | 96.2% | (many diffs) |
| 23 | mouse | 67345 | Herc4 | hect domain and RLD 4 | XM_006513985.1 | 84.9% | 89.4% | (many diffs) |
| 24 | mouse | 67345 | Herc4 | hect domain and RLD 4 | XM_006513987.2 | 80.6% | 85.5% | (many diffs) |
| 25 | mouse | 67345 | Herc4 | hect domain and RLD 4 | XM_006513986.3 | 80% | 84.8% | (many diffs) |
| 26 | mouse | 67345 | Herc4 | hect domain and RLD 4 | XM_006513988.3 | 77.8% | 80.9% | (many diffs) |
| 27 | mouse | 67345 | Herc4 | hect domain and RLD 4 | XR_380431.3 | 72.1% | (many diffs) | |
| 28 | mouse | 67345 | Herc4 | hect domain and RLD 4 | XM_006513990.3 | 69.1% | 73.1% | (many diffs) |
| 29 | mouse | 67345 | Herc4 | hect domain and RLD 4 | XM_006513989.3 | 68.6% | 72.5% | (many diffs) |
| 30 | mouse | 67345 | Herc4 | hect domain and RLD 4 | XR_380432.3 | 50.3% | (many diffs) | |
| 31 | mouse | 67345 | Herc4 | hect domain and RLD 4 | XM_006513991.3 | 47.5% | 49.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3213
- ORF length:
- 3147
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt gtgctgggga aatgcatcct ttgggcagct aggtttgggt ggaattgatg 121 aagaaattgt actagagccc agaaaaagtg acttctttat aaataaaagg gtccgagatg 181 taggatgtgg actcagacat actgtgtttg ttctggatga tggaacagtg tacacatgtg 241 gatgtaatga tctaggacag ctaggtcatg aaaaatccag aaagaaacca gagcaggttg 301 ttgccctgga tgcccaaaat attgtagctg tttcatgtgg agaagctcat acgttagcgc 361 taaatgacaa aggccaggtg tatgcttggg gtctcgattc tgatggacag cttggcctgg 421 taggatcaga ggaatgcatc agagtaccca gaaatattaa aagtttgtca gatatccaga 481 ttgtacaggt tgcttgtggt tactatcatt cacttgcact ttctaaagca agtgaagtct 541 tctgttgggg acagaataaa tatggccaat tgggtttagg tactgactgt aaaaagcaaa 601 cttcaccgca gctgcttaag tctttgcttg gaatcccttt catgcaagtt gcagcaggag 661 gagcccatag ttttgtactc accctttctg gagctatctt tggatgggga cgcaacaagt 721 ttggtcagct aggtcttaat gatgaaaatg ataggtatgt tcctaattta ctaaagtcac 781 taagatctca gaaaatagtt tatatttgtt gtggagaaga tcatactgct gctctaacca 841 aggaaggtgg agtgtttact tttggagctg gagggtatgg tcagttgggc cataattcta 901 ccagtcatga aataaaccca aggaaagttt ttgaacttat gggaagcatt gtcactgaga 961 ttgcttgtgg acggcagcac acttctgctt ttgttccttc atcaggacga atttactctt 1021 ttgggcttgg tggtaatggg cagctgggaa ccggttcaac aagcaacagg aaaagcccct 1081 ttactgtaaa aggaaattgg tacccctata atgggcagtg tctaccagat attgattctg 1141 aagaatattt ctgtgtaaaa agaattttct cagggggaga tcaaagcttt tcacattact 1201 ctagtcccca gaactgtggg ccaccagatg acttcagatg tcccaatccg acaaagcaga 1261 tctggacagt gaatgaagct ctaattcaga aatggctgag ctatccttct ggaaggtttc 1321 ctgtggagat agccaatgag atagatggaa cgttttcttc ctctggttgc ctaaatggaa 1381 gttttttagc tgttagcaat gatgatcact atagaacagg taccagattt tcaggggttg 1441 atatgaatgc tgctaggctt ttattccaca aacttataca acctgatcat ccgcagatat 1501 ctcagcaggt ggcagctagt ttggaaaaga atcttattcc taaactgact agctccttac 1561 ctgatgttga agcattgagg ttttatctta ctctaccaga atgtcccctg atgagtgatt 1621 ccaacaattt cacaacaata gcaattccct ttggtacagc tcttgtgaac ctagaaaagg 1681 caccactgaa agtacttgaa aactggtggt cagtacttga acctccacta ttcctcaaga 1741 tagtagaact ttttaaggaa gttgtggtac atcttttgaa actctacaag atcggtattc 1801 ccccttctga aagaagaatt ttcaacagtt ttcttcatac tgcattaaag gttttagaaa 1861 tactacatag ggtaaatgag aaaatgggac agattataca gtatgataaa ttttatatac 1921 atgaagtaca agaattgata gacataagaa atgattatat caactgggtc caacagcagg 1981 cctatggaat gttggcagat atccctgtta caatctgtac atatccattt gtatttgatg 2041 cccaagcaaa aactactctg ttacagaccg atgcagtctt acagatgcag atggctattg 2101 atcaggccca caggcagaat gtctcctctc tttttctccc agtgattgaa tctgtgaatc 2161 cctgcttaat tctagtggtg cgtagagaaa atattgtagg agatgcaatg gaagtcctta 2221 ggaaaacaaa gaacatagat tacaagaagc cactcaaggt tatatttgtt ggagaagatg 2281 ctgtggatgc aggaggggtg cgcaaagaat ttttcttgct catcatgagg gaattattgg 2341 atcctaaata cggcatgttt aggtattatg aagattccag gctcatttgg ttttctgata 2401 agacatttga agacagtgat ttgttccatt tgattggtgt tatctgtggc ttagcaattt 2461 ataattgtac cattgtggac ctccattttc ctttggcttt atataagaaa ctactgaaaa 2521 agaagccatc cttggatgat ttgaaagaac taatgcctga tgttgggaga agcatgcaac 2581 agttactgga ttatccagaa gatgacatag aggaaacatt ttgtcttaat tttacgatca 2641 cagttgaaaa ctttggtgca acagaagtga aagagctggt tctaaatggt gcagacacag 2701 ctgttaacaa acaaaatcgg caagagtttg tcgatgctta tgtggattac atattcaata 2761 aatcagtggc ttccttattt gatgcttttc atgcgggctt tcataaggtc tgtggaggaa 2821 aagtccttct gctctttcag cctaatgaac tacaagcaat ggtcattgga aatacaaatt 2881 atgattggaa ggaactggaa aagaatacag aatacaaagg ggaatattgg gcagaacatc 2941 ctacgataaa aattttttgg gaagtatttc acgaattacc attggaaaag aagaaacagt 3001 ttctgttatt tttgacaggt agtgatcgca ttcctattct tggtatgaag agtctgaaac 3061 tagtcatcca gtccacagga ggtggtgagg agtatctccc agtttcccat acttgtttta 3121 atcttctgga tcttccaaaa tatacagaaa aagaaactct acgctctaaa ctgatccaag 3181 ctattgatca caatgaaggc ttcagtttaa tatacccaac tttcttgtac aaagtggttg 3241 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 3301 accggtggcg cgttaagtcg acaatcaacc tctggatta