Construct: ORF ccsbBroad304_02929
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000850.1_s304c1, BRDN0000403543
- Derived from:
- ccsbBroadEn_02929
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXW2 (26190)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02929
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26190 | FBXW2 | F-box and WD repeat domain ... | NM_012164.4 | 100% | 100% | |
| 2 | human | 26190 | FBXW2 | F-box and WD repeat domain ... | XM_005251910.4 | 100% | 100% | |
| 3 | human | 26190 | FBXW2 | F-box and WD repeat domain ... | XM_006717049.3 | 93.4% | 93.4% | 1_96del |
| 4 | human | 26190 | FBXW2 | F-box and WD repeat domain ... | XM_011518518.1 | 93.4% | 93.4% | 1_96del |
| 5 | human | 26190 | FBXW2 | F-box and WD repeat domain ... | XM_017014615.2 | 85.6% | 85.6% | 489_490ins195 |
| 6 | human | 26190 | FBXW2 | F-box and WD repeat domain ... | XM_024447509.1 | 85.6% | 85.6% | 489_490ins195 |
| 7 | human | 26190 | FBXW2 | F-box and WD repeat domain ... | XM_006717051.2 | 80% | 80% | 1_96del;585_586ins195 |
| 8 | human | 26190 | FBXW2 | F-box and WD repeat domain ... | XM_005251913.2 | 71.5% | 71.5% | 0_1ins387 |
| 9 | human | 26190 | FBXW2 | F-box and WD repeat domain ... | XM_017014616.1 | 71.5% | 71.5% | 0_1ins387 |
| 10 | human | 26190 | FBXW2 | F-box and WD repeat domain ... | XM_017014617.1 | 71.5% | 71.5% | 0_1ins387 |
| 11 | human | 26190 | FBXW2 | F-box and WD repeat domain ... | XR_001746269.1 | 49.3% | 1_478del;1163_1253del;1521_1522ins410 | |
| 12 | mouse | 30050 | Fbxw2 | F-box and WD-40 domain prot... | NM_001164768.1 | 95.7% | 98.4% | (many diffs) |
| 13 | mouse | 30050 | Fbxw2 | F-box and WD-40 domain prot... | NM_013890.4 | 95.7% | 98.4% | (many diffs) |
| 14 | mouse | 30050 | Fbxw2 | F-box and WD-40 domain prot... | XM_006498117.4 | 88.5% | 91% | (many diffs) |
| 15 | mouse | 30050 | Fbxw2 | F-box and WD-40 domain prot... | NM_001164769.1 | 76.1% | 78.4% | (many diffs) |
| 16 | mouse | 30050 | Fbxw2 | F-box and WD-40 domain prot... | NM_001164770.1 | 67.8% | 70.2% | (many diffs) |
| 17 | mouse | 30050 | Fbxw2 | F-box and WD-40 domain prot... | XM_006498118.4 | 67.8% | 70.2% | (many diffs) |
| 18 | mouse | 30050 | Fbxw2 | F-box and WD-40 domain prot... | NM_001164772.1 | 51.4% | 50.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1428
- ORF length:
- 1362
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gagaaaggac tttgagacat ggcttgataa catttctgtt acatttcttt 121 ctctgacgga cttgcagaaa aatgaaactc tggatcacct gattagtctg agtggggcag 181 tccagctcag gcatctctcc aataacctag agactctcct caagcgggac ttcctcaaac 241 tccttcccct ggagctcagt ttttatttgt taaaatggct cgatcctcag actttactca 301 catgctgcct cgtctctaaa cagtggaata aggtgataag tgcctgtaca gaggtgtggc 361 agactgcatg taaaaatttg ggctggcaga tagatgattc tgttcaggac gctttgcact 421 ggaagaaggt ttatttgaag gctattttga gaatgaagca actggaggac catgaagcct 481 ttgaaacctc gtcattaatt ggacacagtg ccagagtgta tgcactttac tacaaagatg 541 gacttctctg tacagggtca gatgacttgt ctgcaaagct gtgggatgtg agcacagggc 601 agtgcgttta tggcatccag acccacactt gtgcagcggt gaagtttgat gaacagaagc 661 ttgtgacagg ctcctttgac aacactgtgg cttgctggga atggagttcc ggagccagga 721 cccagcactt tcgggggcac acgggggcgg tatttagcgt ggactacaat gatgaactgg 781 atatcttggt gagcggctct gcagacttca ctgtgaaagt atgggcttta tctgctggga 841 catgcctgaa cacactcacc gggcacacgg aatgggtcac caaggtagtt ttgcagaagt 901 gcaaagtcaa gtctctcttg cacagtcctg gagactacat cctcttaagt gcagacaaat 961 atgagattaa gatttggcca attgggagag aaatcaactg taagtgctta aagacattgt 1021 ctgtctctga ggatagaagt atctgcctgc agccaagact tcattttgat ggcaaataca 1081 ttgtctgtag ttcagcactt ggtctctacc agtgggactt tgccagttat gatattctca 1141 gggtcatcaa gactcctgag atagcaaact tggccttgct tggctttgga gatatctttg 1201 ccctgctgtt tgacaaccgc tacctgtaca tcatggactt gcggacagag agcctgatta 1261 gtcgctggcc tctgccagag tacaggaaat caaagagagg ctcaagcttc ctggcaggcg 1321 aagcatcctg gctgaatgga ctggatgggc acaatgacac gggcttggtc tttgccacca 1381 gcatgcctga ccacagtatt cacctggtgt tgtggaagga gcacggctgc ccaactttct 1441 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 1501 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta