Construct: ORF ccsbBroad304_03054

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006384.1_s304c1, BRDN0000406665
Derived from:
ccsbBroadEn_03054
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
IFT81 (28981)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03054
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 28981 IFT81 intraflagellar transport 81 NM_001347946.2 100% 100%
2 human 28981 IFT81 intraflagellar transport 81 NM_031473.4 100% 100%
3 human 28981 IFT81 intraflagellar transport 81 XR_944523.3 74.2% 1_112del;1406_1741del
4 human 28981 IFT81 intraflagellar transport 81 NM_001143779.2 61.1% 57.3% (many diffs)
5 human 28981 IFT81 intraflagellar transport 81 NM_014055.4 61.1% 57.3% (many diffs)
6 human 28981 IFT81 intraflagellar transport 81 XM_017019217.1 61.1% 57.3% (many diffs)
7 human 28981 IFT81 intraflagellar transport 81 XR_001748670.2 59.3% 1_548del;1842_2177del
8 human 28981 IFT81 intraflagellar transport 81 XR_429093.3 45.8% (many diffs)
9 human 28981 IFT81 intraflagellar transport 81 NR_144951.2 43.9% (many diffs)
10 human 28981 IFT81 intraflagellar transport 81 XR_001748672.2 40.3% 1_112del;1150_1271del;1528_3203del
11 human 28981 IFT81 intraflagellar transport 81 NR_144952.2 38.3% (many diffs)
12 human 28981 IFT81 intraflagellar transport 81 NR_144950.2 38.2% (many diffs)
13 human 28981 IFT81 intraflagellar transport 81 NR_144949.2 35.5% (many diffs)
14 mouse 12589 Ift81 intraflagellar transport 81 XM_006530128.2 59.5% 58.7% (many diffs)
15 mouse 12589 Ift81 intraflagellar transport 81 XM_011248162.1 54.4% 53.8% (many diffs)
16 mouse 12589 Ift81 intraflagellar transport 81 NM_009879.3 54.3% 53.7% (many diffs)
17 mouse 12589 Ift81 intraflagellar transport 81 XM_006530127.3 54.3% 53.7% (many diffs)
18 mouse 12589 Ift81 intraflagellar transport 81 XM_006530129.3 44.9% 43.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1359
ORF length:
1293
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag tgatcaaatt aaattcatta tggacagtct caataaggag ccctttagga
121agaactataa tttaatcacg tttgattcct tggagccaat gcaactatta caagttctca
181gtgatgttct ggctgagatt gacccaaagc aacttgtgga tatcagagag gagatgccag
241agcagacagc caaacgaatg ttgagccttc ttggtattct taagtacaaa ccttcaggaa
301atgccacaga tatgagtact tttcgtcagg gtttggtgat tggaagtaaa cctgtaattt
361acccagtgct ccactggctt cttcagagga ctaatgaact gaagaaaaga gcatatttag
421ctcgtttttt aataaaactt gaggtaccaa gtgagtttct tcaggatgaa actgtggctg
481acaccaataa acagtatgaa gagttaatgg aagcctttaa aactttgcat aaagaatatg
541agcagctcaa gatatctgga ttttctacag cagaaataag aaaggatatc agtgcaatgg
601aagaagaaaa ggatcagctc attaagagag ttgaacattt gaagaaaagg gttgagacag
661ctcagaatca tcaatggatg cttaaaatag caaggcaact tcgagttgaa aaagagagag
721aagaatatct tgcacaacag aaacaggaac aaaagaatca gctatttcat gcagtgcaaa
781gattgcaaag agtacaaaac cagctgaaaa gcatgcgcca agctgcagca gatgcaaagc
841ctgaaagttt aatgaagagg ctagaggagg agataaaatt taatttatat atggtaactg
901aaaaatttcc taaagaatta gaaaataaga aaaaggaatt acatttttta caaaaagtag
961tttcagagcc agctatgggc cattctgatc ttcttgaact tgaatctaaa ataaatgaaa
1021taaacacaga aattaaccag ttgattgaaa agaaaatgat gagaaatgag cccattgaag
1081gcaaactctc actgtatagg caacaggcat ctatcatttc ccgtaaaaaa gaagccaaag
1141ctgaggaact tcaggaggcc aaggagaagt tagccagcct agagagagaa gcatcagtaa
1201agagaaatca gacccgtgaa tttgatggta ctgaagtttt aaagggagat gagagacagg
1261atctcactct gtcacccagg ctggagtgcg gtggtgtgat catggcttac tgcagcctga
1321aactcctagg ctcaagtgat cctcctacct cagcctcctg cccaactttc ttgtacaaag
1381tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1441gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

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