Construct: ORF ccsbBroad304_03116

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002023.1_s304c1, BRDN0000462245
Derived from:
ccsbBroadEn_03116
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TRHDE (29953)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03116
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29953 TRHDE thyrotropin releasing hormo... NM_013381.2 100% 100%
2 human 29953 TRHDE thyrotropin releasing hormo... XM_017019243.2 94.4% 92.4% (many diffs)
3 human 29953 TRHDE thyrotropin releasing hormo... XM_005268819.5 71.4% 71.1% (many diffs)
4 human 29953 TRHDE thyrotropin releasing hormo... XM_017019244.1 70.4% 70.4% 0_1ins909
5 human 29953 TRHDE thyrotropin releasing hormo... XM_011538248.2 60.4% 60.4% 0_1ins1215
6 mouse 237553 Trhde TRH-degrading enzyme NM_146241.2 90% 95.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3141
ORF length:
3072
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gggggaagac gacgccgcgc ttcgggctgg cagcaggggg ctctccgacc
121cgtgggcaga ctcagtggga gtgcgacccc gcaccacgga gcgccacatc gccgtacaca
181agcggcttgt gctggccttc gctgtgtccc tcgtggcatt gctcgcggtc acaatgctcg
241ctgtgctgct cagcctgcgc ttcgacgagt gcggggcgag tgccacgcca ggcgccgacg
301gtggcccctc aggctttccg gagcgcggcg gcaacgggag cctccctgga tcggcccggc
361gcaaccacca cgcaggcggg gactcctggc agcccgaggc gggtggggtg gccagtccgg
421ggaccacgtc ggcccagccg ccgtcggagg aggagcggga gccgtgggag ccgtggacgc
481agctgcgcct gtcgggccac ctgaagccgc tgcactacaa tctgatgctc accgccttca
541tggagaactt caccttctcc ggggaggtca acgtggagat cgcgtgccgg aacgccaccc
601gctacgtagt gctgcacgct tcccgagtgg cggtggagaa agtgcagctg gccgaggacc
661gggcgttcgg ggctgtccct gtagccggtt ttttcctcta cccgcaaacc caggtcttag
721tggtggtgct gaataggaca ctggacgcgc agaggaatta caatctgaag attatctaca
781acgcgctcat cgagaatgag ctcctgggct tcttccgcag ctcctatgtg ctccacgggg
841agagaagatt ccttggtgtt actcagtttt cgcctacaca tgccagaaag gcatttcctt
901gttttgatga gccaatctac aaggctactt tcaaaatcag catcaagcat caagcaacct
961atttatcttt atctaatatg ccagtggaaa cttccgtgtt tgaggaagat ggatgggtta
1021cggatcactt ttcacagacc cctctcatgt ccacatatta tttagcctgg gcaatttgca
1081acttcacata cagagaaact accaccaaga gtggggttgt agtacgatta tatgcaagac
1141ctgatgctat cagaagagga tccggggact atgctctcca tataacaaag agattaatag
1201aattttatga agactacttt aaagtgccct attccttgcc aaaactagat cttttagctg
1261tgcctaagca tccgtatgct gctatggaga actggggact aagtattttt gtggaacaaa
1321gaatactgct ggatcccagt gtttcatcta tttcttattt gctggatgtc accatggtca
1381ttgttcatga gatatgtcac cagtggtttg gtgaccttgt gacgcctgtg tggtgggaag
1441acgtgtggct gaaggaaggg tttgctcact actttgaatt tgttggtaca gactacctct
1501atcctggctg gaacatggaa aagcagaggt ttctgaccga tgttctgcat gaagtgatgc
1561tgctggacgg tttggccagt tcccatccag tatcacagga agtgctgcag gcaacagata
1621ttgacagggt gtttgactgg atcgcatata aaaagggtgc tgctttaata agaatgctgg
1681ctaattttat gggccattca gttttccaga ggggtttgca agattattta accattcata
1741agtatggtaa tgcagccaga aatgatctct ggaatacatt atcggaggct ttaaaaagaa
1801atgggaaata tgtaaatata caagaagtaa tggatcagtg gacactccag atgggttatc
1861ctgttatcac catcttggga aacacaacag cagaaaatag aataataatt acccaacagc
1921attttatcta tgatatcagt gctaaaacta aagcacttaa acttcagaat aacagttacc
1981tgtggcagat tccattaact attgtggtag gaaatagaag ccatgtgtct tcagaagcaa
2041ttatttgggt gtctaacaaa tcagagcacc acagaataac ttatttggac aaaggaagct
2101ggctgctggg gaacatcaat caaactggct attttagagt caactatgac ctaaggaact
2161ggagattatt aattgatcaa ttaatccgga atcatgaggt tctttctgtc agtaaccgag
2221cgggcttgat cgatgatgcc ttcagcctag ccagggctgg ctatttgcct cagaatattc
2281ctctggagat tatcagatac ctgtctgagg agaaggattt tcttccttgg catgctgcca
2341gccgagctct ttatcctcta gataaattac tggaccgcat ggaaaactac aacattttca
2401atgaatatat tttaaagcaa gttgcaacaa catatatcaa gcttgggtgg ccgaaaaata
2461attttaatgg atctcttgtt caagcatcct accaacatga agaactacgt agagaagtta
2521taatgctggc ctgcagtttt ggcaacaagc actgtcacca acaggcatca acacttattt
2581cagattggat ttccagcaac aggaacagaa taccactaaa tgttagagac atcgtatact
2641gtacaggagt gtcactactg gatgaggatg tctgggaatt catatggatg aaattccatt
2701ccaccacagc agtttctgag aagaaaatat tattggaagc cttaacttgc agtgatgaca
2761ggaatttatt aaacaggctt ctaaatctgt cactgaattc tgaggtggtg ctggatcaag
2821atgcaattga tgtcataatc catgtagctc gaaatccaca tggtcgagac cttgcctgga
2881agtttttcag ggataaatgg aagatattaa ataccaggta tggagaagca ttgtttatga
2941attccaaact catcagtggt gtcacagaat ttcttaatac tgaaggtgaa ctcaaagagc
3001tcaagaactt catgaaaaac tatgatgggg tagctgctgc ttctttctca cgagctgtgg
3061aaactgtcga agccaatgtg cgctggaaaa tgctttacca agacgagctt ttccaatggt
3121taggaaaagc tctaagacac ttgccaactt tcttgtacaa agtggttggt aagcctatcc
3181ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
3241ttaagtcgac aatcaacctc tggatta

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