Construct: ORF ccsbBroad304_03298
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013221.1_s304c1, BRDN0000410970
- Derived from:
- ccsbBroadEn_03298
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AIG1 (51390)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03298
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51390 | AIG1 | androgen induced 1 | NM_016108.4 | 100% | 100% | |
| 2 | human | 51390 | AIG1 | androgen induced 1 | NM_001366350.1 | 98.7% | 98.3% | 679_680insGTATGGAAG |
| 3 | human | 51390 | AIG1 | androgen induced 1 | NM_001366351.1 | 98% | 97% | 4A>G;7_7delAinsCTTGTCCCCTGCC |
| 4 | human | 51390 | AIG1 | androgen induced 1 | NM_001366348.1 | 97.5% | 97.1% | 680_697del |
| 5 | human | 51390 | AIG1 | androgen induced 1 | NM_001363721.2 | 96.3% | 94.9% | (many diffs) |
| 6 | human | 51390 | AIG1 | androgen induced 1 | NM_001286587.1 | 95.7% | 95.7% | 0_1ins30 |
| 7 | human | 51390 | AIG1 | androgen induced 1 | NM_001366349.2 | 95.3% | 92.6% | (many diffs) |
| 8 | human | 51390 | AIG1 | androgen induced 1 | NM_001366347.1 | 94.6% | 90.4% | (many diffs) |
| 9 | human | 51390 | AIG1 | androgen induced 1 | NM_001366346.1 | 91.8% | 92.7% | (many diffs) |
| 10 | human | 51390 | AIG1 | androgen induced 1 | NM_001366345.1 | 90% | 88.9% | (many diffs) |
| 11 | human | 51390 | AIG1 | androgen induced 1 | NM_001366353.1 | 85.7% | 85.7% | 297_298ins102 |
| 12 | human | 51390 | AIG1 | androgen induced 1 | NM_001366344.1 | 83.5% | 83.5% | 141_281del |
| 13 | human | 51390 | AIG1 | androgen induced 1 | NM_001366354.1 | 82.3% | 62.5% | 396_397ins116;588_589insTAAATTGGAA |
| 14 | human | 51390 | AIG1 | androgen induced 1 | NM_001366355.1 | 78.1% | 78.1% | 141_142ins156 |
| 15 | human | 51390 | AIG1 | androgen induced 1 | NM_001366357.1 | 74.1% | 73.1% | (many diffs) |
| 16 | human | 51390 | AIG1 | androgen induced 1 | NM_001366352.1 | 74% | 55% | (many diffs) |
| 17 | human | 51390 | AIG1 | androgen induced 1 | NM_001366358.1 | 63% | 63% | 0_1ins264 |
| 18 | human | 51390 | AIG1 | androgen induced 1 | NM_001286589.1 | 56.6% | 56.3% | (many diffs) |
| 19 | human | 51390 | AIG1 | androgen induced 1 | NM_001286588.2 | 56% | 55.8% | 401delT;402_403ins313 |
| 20 | human | 51390 | AIG1 | androgen induced 1 | NM_001366359.1 | 54.6% | 53.3% | (many diffs) |
| 21 | human | 51390 | AIG1 | androgen induced 1 | NM_001366361.1 | 48.4% | 45% | (many diffs) |
| 22 | human | 51390 | AIG1 | androgen induced 1 | NM_001366356.1 | 47.3% | 47% | (many diffs) |
| 23 | human | 51390 | AIG1 | androgen induced 1 | NR_158960.1 | 28.7% | (many diffs) | |
| 24 | human | 51390 | AIG1 | androgen induced 1 | NR_158896.1 | 27% | (many diffs) | |
| 25 | human | 51390 | AIG1 | androgen induced 1 | NM_001366363.1 | 19.7% | 19.7% | 141_142ins573 |
| 26 | human | 51390 | AIG1 | androgen induced 1 | NM_001366362.1 | 17.1% | 16% | (many diffs) |
| 27 | mouse | 66253 | Aig1 | androgen-induced 1 | NM_001347362.1 | 89.2% | 95.3% | (many diffs) |
| 28 | mouse | 66253 | Aig1 | androgen-induced 1 | NM_025446.4 | 79.8% | 81.2% | (many diffs) |
| 29 | mouse | 66253 | Aig1 | androgen-induced 1 | XM_006512812.3 | 62.9% | 62.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 780
- ORF length:
- 714
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gcttgtcccc tgccaggtgc tgcggatggc aatcctgctg tcttactgct 121 ctatcctgtg taactacaag gccatcgaaa tgccctcaca ccagacctac ggagggagct 181 ggaaattcct gacgttcatt gatctggtta tccaggctgt cttttttggc atctgtgtgc 241 tgactgatct ttccagtctt ctgactcgag gaagtgggaa ccaggagcaa gagaggcagc 301 tcaagaagct catctctctc cgggactgga tgttagctgt gttggccttt cctgttgggg 361 tttttgttgt agcagtgttc tggatcattt atgcctatga cagagagatg atatacccga 421 agctgctgga taattttatc ccagggtggc tgaatcacgg aatgcacacg acggttctgc 481 cctttatatt aatcgagatg aggacatcgc accatcagta tcccagcagg agcagcggac 541 ttaccgccat atgtaccttc tctgttggct atatattatg ggtgtgctgg gtgcatcatg 601 taactggcat gtgggtgtac cctttcctgg aacacattgg cccaggagcc agaatcatct 661 tctttgggtc tacaaccatc ttaatgaact tcctgtacct gctgggagaa gttctgaaca 721 actatatctg ggatacacag aaaagtatgg aagaagagaa agaaaagcct aaattggaat 781 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 841 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 901 ggatta