Construct: ORF ccsbBroad304_03311
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007682.1_s304c1, BRDN0000403518
- Derived from:
- ccsbBroadEn_03311
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EVL (51466)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03311
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51466 | EVL | Enah/Vasp-like | NM_016337.3 | 100% | 100% | |
| 2 | human | 51466 | EVL | Enah/Vasp-like | NM_001330221.2 | 99.5% | 99.2% | 3_4insGCCACA |
| 3 | human | 51466 | EVL | Enah/Vasp-like | XM_005267749.3 | 98.7% | 98.3% | 4_7delTTTGinsGCCA;10_21del |
| 4 | human | 51466 | EVL | Enah/Vasp-like | XM_011536828.2 | 80.5% | 73.3% | (many diffs) |
| 5 | human | 51466 | EVL | Enah/Vasp-like | XM_017021363.2 | 79.6% | 65.4% | (many diffs) |
| 6 | human | 51466 | EVL | Enah/Vasp-like | XR_001750362.1 | 48.5% | (many diffs) | |
| 7 | human | 51466 | EVL | Enah/Vasp-like | XR_001750364.1 | 35.1% | (many diffs) | |
| 8 | human | 51466 | EVL | Enah/Vasp-like | XR_001750366.1 | 34.7% | (many diffs) | |
| 9 | human | 51466 | EVL | Enah/Vasp-like | XR_001750361.1 | 34.4% | (many diffs) | |
| 10 | human | 51466 | EVL | Enah/Vasp-like | XR_001750356.1 | 34% | (many diffs) | |
| 11 | human | 51466 | EVL | Enah/Vasp-like | XR_001750359.2 | 33.5% | (many diffs) | |
| 12 | human | 51466 | EVL | Enah/Vasp-like | XR_001750367.1 | 33.4% | (many diffs) | |
| 13 | human | 51466 | EVL | Enah/Vasp-like | XR_001750357.2 | 31.6% | (many diffs) | |
| 14 | human | 51466 | EVL | Enah/Vasp-like | XR_001750360.1 | 29.7% | (many diffs) | |
| 15 | human | 51466 | EVL | Enah/Vasp-like | XR_001750363.1 | 29.7% | (many diffs) | |
| 16 | human | 51466 | EVL | Enah/Vasp-like | XR_001750355.1 | 28.8% | (many diffs) | |
| 17 | human | 51466 | EVL | Enah/Vasp-like | XR_002957557.1 | 28.2% | (many diffs) | |
| 18 | mouse | 14026 | Evl | Ena-vasodilator stimulated ... | XM_006515471.1 | 89.1% | 93.3% | (many diffs) |
| 19 | mouse | 14026 | Evl | Ena-vasodilator stimulated ... | NM_001163394.1 | 89% | 93.3% | (many diffs) |
| 20 | mouse | 14026 | Evl | Ena-vasodilator stimulated ... | XM_006515470.1 | 88.4% | 92.4% | (many diffs) |
| 21 | mouse | 14026 | Evl | Ena-vasodilator stimulated ... | NM_001163396.2 | 86% | 90.4% | (many diffs) |
| 22 | mouse | 14026 | Evl | Ena-vasodilator stimulated ... | NM_007965.3 | 83.9% | 88.2% | (many diffs) |
| 23 | mouse | 14026 | Evl | Ena-vasodilator stimulated ... | NM_001163395.1 | 83.4% | 87.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1320
- ORF length:
- 1254
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cacaagtgaa cagagtatct gccaagcccg ggcttccgtg atggtctacg 121 atgacaccag taagaaatgg gtaccaatca aacctggcca gcagggattc agccggatca 181 acatctacca caacactgcc agcaacacct tcagagtcgt tggagtcaag ttgcaggatc 241 agcaggttgt gatcaattat tcaatcgtga aagggctgaa gtacaatcag gccacgccaa 301 ccttccacca gtggcgagat gcccgccagg tctacggctt aaactttgca agtaaagaag 361 aggcaaccac gttctccaat gcaatgctgt ttgccctgaa catcatgaat tcccaagaag 421 gaggcccctc cagccagcgt caggtgcaga atggcccctc tcctgatgag atggacatcc 481 agagaagaca agtgatggag cagcaccagc agcagcgtca ggaatctcta gaaagaagaa 541 cctcggccac agggcccatc ctcccaccag gacatccttc atctgcagcc agcgcccccg 601 tctcatgtag tgggcctcca ccgccccccc cacccccagt cccacctcca cccactgggg 661 ctaccccacc tcccccaccc ccactgccag ccggaggagc ccaggggtcc agccacgacg 721 agagctccat gtcaggactg gccgctgcca tagctggggc caagctgaga agagtccaac 781 ggccagaaga cgcatctgga ggctccagtc ccagtgggac ctcaaagtcc gatgccaacc 841 gggcaagcag cgggggtggc ggaggaggcc tcatggagga aatgaacaaa ctgctggcca 901 agaggagaaa agcagcctcc cagtcagaca agccagccga gaagaaggaa gatgaaagcc 961 aaatggaaga tcctagtacc tccccctctc cggggacccg agcagccagc cagccaccta 1021 actcctcaga ggctggccgg aagccctggg agcggagcaa ctcggtggag aagcctgtgt 1081 cctcgattct gtccagaacc ccgtctgtgg caaagagccc cgaagctaag agcccccttc 1141 agtcgcagcc tcactctagg atgaagcctg ctgggagcgt gaatgacatg gccctggatg 1201 ccttcgactt ggaccggatg aagcaggaga tcctagagga ggtggtgaga gagctccaca 1261 aggtgaagga ggagatcatc gacgccatca ggcaggagct gagtgggatc agcaccacgt 1321 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1381 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1441 ggatta