Transcript: Human NM_016337.3

Homo sapiens Enah/Vasp-like (EVL), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
EVL (51466)
Length:
1834
CDS:
80..1336

Additional Resources:

NCBI RefSeq record:
NM_016337.3
NBCI Gene record:
EVL (51466)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_016337.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000063871 CGGCTTAAACTTTGCAAGTAA pLKO.1 349 CDS 100% 5.625 7.875 N EVL n/a
2 TRCN0000286943 CGGCTTAAACTTTGCAAGTAA pLKO_005 349 CDS 100% 5.625 7.875 N EVL n/a
3 TRCN0000063872 TGTCCTCGATTCTGTCCAGAA pLKO.1 1092 CDS 100% 4.050 5.670 N EVL n/a
4 TRCN0000286941 TGTCCTCGATTCTGTCCAGAA pLKO_005 1092 CDS 100% 4.050 5.670 N EVL n/a
5 TRCN0000294336 ACGATGACACCAGTAAGAAAT pLKO_005 132 CDS 100% 13.200 9.240 N EVL n/a
6 TRCN0000063868 CCCTAAGTCACCTGCTTCATT pLKO.1 1618 3UTR 100% 5.625 3.938 N EVL n/a
7 TRCN0000294298 ACACCAGGTCTGCTCGTCTTT pLKO_005 1542 3UTR 100% 4.950 3.465 N EVL n/a
8 TRCN0000063869 GCCAAATGGAAGATCCTAGTA pLKO.1 972 CDS 100% 4.950 3.465 N EVL n/a
9 TRCN0000286942 GCCAAATGGAAGATCCTAGTA pLKO_005 972 CDS 100% 4.950 3.465 N EVL n/a
10 TRCN0000063870 CCGGATCAACATCTACCACAA pLKO.1 187 CDS 100% 4.050 2.835 N EVL n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_016337.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03311 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_03311 pLX_304 0% 100% 100% V5 n/a
3 ccsbBroadEn_10459 pDONR223 100% 99.5% 99.2% None 4_9delGCCACA n/a
4 ccsbBroad304_10459 pLX_304 0% 99.5% 99.2% V5 4_9delGCCACA n/a
5 TRCN0000471524 TTTACTACCAGAGTAGATCTTTTT pLX_317 17.9% 99.5% 99.2% V5 4_9delGCCACA n/a
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